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Transdifferentiation of periodontal ligament stem cells into acinar cells using an indirect co-culture system

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Abstract

Serous Acinar Cells (ACs) are mature and functional secretory epithelial cells that develop and complete through other stem cells at the end of the ductal system. So, the regeneration of the salivary gland damaged by radiation does not occur without cell therapy. Todays, an accessible tissue like the Periodontal Ligament (PDL) of the tooth was considered to easily extract the Mesenchymal Stem Cells (MSCs). In-vitro differentiation of stem cells before transplantation to damaged tissue reduces the risk of tumorigenesis. This study was conducted to evaluate the feasibility of differentiation of PDLSCs into salivary acinar cells by a co-culture system. PDLSCs were isolated from adult human PDL tissue and co-cultured with rat parotid ACs using an indirect co-culture system. The transdifferentiation of PDLSCs was evaluated by PCR of Aquaporin 5 (AQP5) and Carbonic anhydrase 6 (CA6) genes, then quantitative real-time PCR was used to measure the gene expression levels. The data were analyzed by ANOVA. Specific bond with the correct size on 6% acrylamide gel and TBE5X buffer showed the expression of AQP5 and CA6 in PDLSCs co-cultured with acinar cells. RT-PCR revealed co-cultured PDLSCs with or without KGF (Keratinocyte Growth Factor) showed significantly increased expression of AQP5 genes in compared to the initial PDLSCs. Expression of AQP5 and CA6, indicating successful transdifferentiation of PDLSCs into ACs, in co-culture system for 3 weeks.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

This research has been supported and funded by Vice-Chancellor in Research Affaire, School of dentistry, Tehran University of Medical Science, Tehran, Iran. Grant number: 98-02-69-42401.

Funding

This study funded by Vice-Chancellor in Research Affairs, School of Dentistry, Tehran University of Medical Science, Tehran, Iran.

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Authors and Affiliations

  1. Oral and Maxillofacial Medicine, School of Dentistry, Tehran University of Medical Science, North Kargar St, P.O. BOX: 14395-433, Tehran, 14399-55991, Iran

    Nafiseh Sheykhbahaei & Maryam Koopaie

  2. School of Dentistry, Tehran University of Medical Science, Tehran, Iran

    Fatemeh Bayramzadeh

Authors
  1. Nafiseh Sheykhbahaei
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  2. Fatemeh Bayramzadeh
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  3. Maryam Koopaie
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Contributions

NS Substantial contribution to the conception and design, Drafting the manuscript, critically revising the manuscript for important intellectual content, Approval of the final version submitted for publication. “Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.” FB Substantial contribution to the acquisition, analysis of data, critically revising the manuscript for important intellectual content, Approval of the final version submitted for publication. “Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.” MK Substantial contribution to the analysis and interpretation of data, Drafting the manuscript, Approval of the final version submitted for publication. “Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.”

Corresponding author

Correspondence to Maryam Koopaie.

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The authors declare that there is no conflict of interests related to this study.The authors have no conflict of interest to declare.

Ethics approval and consent to participate

The protocol of this study was approved by the ethics committee of Tehran University of Medical Science (IR.TUMS.VCR.REC.1399.482). Informed consents were obtained from all individual participants who took part in this study. Also, informed consent were taken from a parent and/or legal guardian for human participants that are minors. All procedures which were involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Human or animal rights

The protocol of this study was approved by the ethics committee of Tehran University of Medical Science (IR.TUMS.VCR.REC.1399.482) and this study was conducted in accordance with the Declaration of Helsinki.

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Sheykhbahaei, N., Bayramzadeh, F. & Koopaie, M. Transdifferentiation of periodontal ligament stem cells into acinar cells using an indirect co-culture system. Cell Tissue Bank 24, 241–251 (2023). https://doi.org/10.1007/s10561-022-10029-1

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  • DOI: https://doi.org/10.1007/s10561-022-10029-1

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