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The time has come to extend the expiration limit of cryopreserved allograft heart valves

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Abstract

Despite the wide choice of commercial heart valve prostheses, cryopreserved semilunar allograft heart valves (C-AHV) are required, and successfully transplanted in selected groups of patients. The expiration limit (EL) criteria have not been defined yet. Most Tissue Establishments (TE) use the EL of 5 years. From physiological, functional, and surgical point of view, the morphology and mechanical properties of aortic and pulmonary roots represent basic features limiting the EL of C-AHV. The aim of this work was to review methods of AHV tissue structural analysis and mechanical testing from the perspective of suitability for EL validation studies. Microscopic structure analysis of great arterial wall and semilunar leaflets tissue should clearly demonstrate cells as well as the extracellular matrix components by highly reproducible and specific histological staining procedures. Quantitative morphometry using stereological grids has proved to be effective, as the exact statistics was feasible. From mechanical testing methods, tensile test was the most suitable. Young’s moduli of elasticity, ultimate stress and strain were shown to represent most important AHV tissue mechanical characteristics, suitable for exact statistical analysis. C-AHV are prepared by many different protocols, so as each TE has to work out own EL for C-AHV.

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Funding

This study was funded by the Project LO1506 of the Czech Ministry of Education, Youth and Sports under the program NPU I, from European Regional Development Fund-Project “Application of Modern Technologies in Medicine and Industry” (No. CZ.02.1.01/0.0/0.0/17_048/0007280), by the National Sustainability Program I (NPU I) Nr. LO1503, and by the Charles University Research Fund—Progres Q39.

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Correspondence to Petra Kochová.

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Burkert, J., Kochová, P., Tonar, Z. et al. The time has come to extend the expiration limit of cryopreserved allograft heart valves. Cell Tissue Bank 22, 161–184 (2021). https://doi.org/10.1007/s10561-020-09843-2

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  • DOI: https://doi.org/10.1007/s10561-020-09843-2

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