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Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview

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Abstract

Cryopreservation is a useful approach to preserve male fertility for assisted reproduction technique and other evaluations. In spite of extensive development in the cryopreservation field, there are biological and biochemical points including DNA fragmentation and antioxidant which should be illuminated to preserve fertility in safe form. Several molecular markers such as coding and noncoding RNAs are transferred from spermatozoa into oocyte via fertilization. These biomarkers affect fertility potential during the cryopreservation. Despite its impact, sperm cryopreservation has some destructive effect including loss of sperm motility and viability, acrosomal damage, mitochondrial membrane depolarization, plasma membrane permeability alteration even nuclear, and DNA damage. There is a growing concern about the impact of sperm cryopreservation on biological factors which can interrupt successful fertility procedures such as in vitro fertilization. Additionally, cryo-damage can decrease embryonic development extent. Promoting cryopreservation method via investigating the wide range of non-invasive factors may be increasingly important to have access to safe approach of freezing. Therefore, the aim of this study is the assessment of biological factors which can penetrate the fertility potential during the freezing procedure, explicate innovative methods in fertility preservation.

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Acknowledgements

This article is in line with Dr. Maryam Ezzati’s thesis about the effect of cryopreservation on male fertility to acquire a Ph.D. in Tabriz Medical University. This study was endorsed by Immunology Research Center, Tabriz University of Medical Sciences, Tabriz/Iran (60961).

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Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Maryam Ezzati & Dariush Shanehbandi

  2. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Maryam Ezzati & Maryam Pashaiasl

  3. Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, P.O. Box 51376563833, Tabriz, Iran

    Kobra Hamdi & Maryam Pashaiasl

  4. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sara Rahbar

  5. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Maryam Pashaiasl

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  1. Maryam Ezzati
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Correspondence to Maryam Pashaiasl.

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Ezzati, M., Shanehbandi, D., Hamdi, K. et al. Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview. Cell Tissue Bank 21, 1–15 (2020). https://doi.org/10.1007/s10561-019-09797-0

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