Abstract
Regeneration of periodontal tissues is affected by the biological and morphological characteristics of the membrane surface. The current study evaluated the adhesion of human gingival fibroblasts (HGF) and MG-63 osteoblast-like cells to Membranes, with and without activated PRP. The line of human gingival fibroblast cells and MG-63 osteoblast-like cells were first prepared and cultured on three types of membranes, including Jason, CenoMembrane and TXT-200 in three groups (FBS 10%, FBS 0.5% and activated PRP). Cell viability was investigated by MTT assay and electron microscopy (SEM) was used to evaluate the cell morphology and adhesion on these membranes after 24 and 72 h. Two-way ANOVA was carried out at the significance level of 0.05. The highest adhesion in the 10% FBS group for HGF and The MG-63 osteoblast-like cells was observed to the Jason membrane during 24 h and 72 h (p < 0.05). However, there were no significant differences among the three membranes in PRP and FBS groups for HGF during 24 h and for MG-63 cells during 72 h (p > 0.05). Activated PRP had a positive effect on the viability and adhesion of both human gingival fibroblasts and osteoblast-like cells as compared to the FBS 0.5% group, but these effects were not as 10% FBS group. The results also showed that Jason membrane had the highest amount of cell viability and adhesion.
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Acknowledgements
This article is based on the Thesis No. 822 written by Dr Karamshahi under supervision of Dr Vahabi and related to the School of Dentistry, Shahid Beheshti University of Medical Sciences. The authors would like to express their gratitude to the Cellular Molecular Oral Biology Laboratory of School of Dentistry, Shahid Beheshti University of Medical Sciences for providing technical support for this study.
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This study was funded by Shahid Beheshti University of Medical Sciences.
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Platelet-rich plasma was obtained from volunteers, after explaining the objective of the study and providing a summary of the research stages as well as obtaining their informed verbal and written consent. Gingival fibroblasts and osteoblast-like cells were also prepared from Cell Bank Department of Pasteur institute. Also, the methodology of this experimental study was consistent to all the ethical protocols approved by the University’s Ethics Committee and registered with the code of IR.SBMU.RIDS.REC.1396.440 at the Ethics Committee.
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Informed verbal and written consent was obtained from volunteers for extracting platelet-rich plasma. Also, the methodology of this experimental study was consistent to all the ethical protocols approved by the University’s Ethics Committee and registered with the code of IR.SBMU.RIDS.REC.1396.440 at the Ethics Committee.
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Vahabi, S., Yadegary, Z. & Karamshahi, M. Evaluating the adhesion of human gingival fibroblasts and MG-63 osteoblast-like cells to activated PRP-coated membranes. Cell Tissue Bank 20, 339–349 (2019). https://doi.org/10.1007/s10561-019-09772-9
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DOI: https://doi.org/10.1007/s10561-019-09772-9