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Construction and biocompatibility of a thin type I/II collagen composite scaffold

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Abstract

Articular cartilage injury is a common type of damage observed in clinical practice. A matrix-induced autologous chondrocyte implant was developed to repair articular cartilage as an advancement on the autologous chondrocyte implant procedure. Here, we establish a thin double layer of collagen as a novel and effective bioscaffold for the regeneration of cartilaginous lesions. We created a collagen membrane with double layers using a cover slip, a cover slip, and the collagen was then freeze-dried under vacuum. Carbodiimide as a crosslinking agent was used to obtain a relatively stable collagen construction. The thickness of the knee joint cartilage from grown rabbits was measured from a frozen section. Both type I and type II collagens were characterized using Sodium dodecylsulfate/polyacrylamide gel electrophoresis (SDS-PAGE) and ultraviolet absorption peaks. The aperture size of the scaffold was observed using a scanning electron microscope (SEM). The degradation of the scaffolds in vitro was tested through digestion using collagenase solution. The mechanical capacity of the scaffolds was assessed under dynamic compression. The influence of the scaffold on chondrocyte proliferation was assessed using the methyl thiazolyl tetrazolium (MTT) colourimetric assay and scanning electron microscopy. The frozen sections of the rabbit femoral condyle showed that the thickness of the weight-bearing area of the articular cartilage was less than 1 mm. The results of the SDS-PAGE and ultraviolet absorption peaks of the collagens were in agreement with the standard photographs in the references. SEM showed that the aperture size of the cross-linked scaffold was 82.14 ± 15.70 μm. The in vitro degradation studies indicated that Carbodiimide cross-linking can effectively enhance the biostability of the scaffolds. The Carbodiimide cross-linking protocol resulted in a mean value for the samples that ranged from 8.72 to 15.95 MPa for the compressive strength. The results of the MTT demonstrated that the scaffold had promoted chondrocyte proliferation and SEM observations showed that the scaffold was a good adhesive and growth material for chondrocytes. Thin type I/II collagen composite scaffold can meet the demands of cartilage tissue engineering and have good biocompatibility.

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Acknowledgements

There are no funding sources to report for this submission. Special thanks to Li-dong Zhou and Ai-bing Liu of the central laboratory of the General Hospital of the Chinese People’s Armed Police Forces. The author thanks Lin Wang from Jinzhou Medical University for her help during the experiment.

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Authors and Affiliations

  1. Orthopedics Department, Changzhou Second People’s Hospital, Changzhou, 213000, Jiangsu Province, China

    Long Han

  2. Orthopedics 4th Department, General Hospital of Chinese People’s Armed Police Forces, Beijing, 100039, China

    Zhong-wen Zhang

  3. Dermatological Department, Children’s Hospital of Xuzhou, Xuzhou, 221006, Jiangsu Province, China

    Bo-he Wang

  4. Graduate School, Shaanxi University of Chinese Medicine, Xianyang, 712000, Shaanxi Province, China

    Zhen-kun Wen

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  1. Long Han
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Correspondence to Long Han.

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Han, L., Zhang, Zw., Wang, Bh. et al. Construction and biocompatibility of a thin type I/II collagen composite scaffold. Cell Tissue Bank 19, 47–59 (2018). https://doi.org/10.1007/s10561-017-9653-2

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