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Tissue engineering for neurodegenerative diseases using human amniotic membrane and umbilical cord

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Abstract

Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton’s jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration. The hAMSC and hAESC obtained from HAM and the hWJMSC from umbilical cords were cultured in induction medium to obtain neural-like cells. The morphological differentiation of hAMSC, hAESC and hWJMSC into neural-like cells was evident after 4–5 days, when they acquired an elongated and multipolar shape, and at 21 days, when they expressed neural and glial markers. On other way, the HAM was completely decellularized without affecting the components of the basement membrane or the matrix. Subsequently, hAMSC, hAESC and hWJMSC differentiated into neural-like cells were seeded onto the decellularized HAM, maintaining their morphology. Finally, conditioned media from the HAM allowed proliferation of hAMSC, hAESC and hWJMSC differentiated to neural-like cells. Both HAM and umbilical cord are biomaterials with great potential for use in regenerative medicine for the treatment of neurodegenerative diseases.

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Acknowledgements

We are grateful to Marta García for technical assistance. We appreciate the collaboration of “Servicio de Ginecología” of CHUAC providing us HAMs and umbilical cords. We would like to thank Biobank of “A Coruña” (XXIAC-INIBIC) for providing us the technical, ethical and legal advice necessary for the development of our research.

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Authors and Affiliations

  1. Unidad de Criobiología-Banco de Tejidos, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain

    Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, Jacinto Sanchez-Ibañez & María Esther Rendal-Vázquez

  2. Servicio de Anatomía Patológica, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain

    Jorge Pombo-Otero

  3. Biobanco A Coruña, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain

    María Fraga-Mariño & Nieves Doménech

  4. Grupo NEUROVER, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de A Coruña (UDC), Campus de A Coruña, 15071, A Coruña, Spain

    María Jesús Manso-Revilla & Antonio Manuel Castro-Castro

  5. Servicio de Cirugía General y del Aparato Digestivo, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain

    Namibia Sanluis-Verdes

Authors
  1. Anahí Sanluis-Verdes
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  2. Namibia Sanluis-Verdes
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  3. María Jesús Manso-Revilla
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  5. Jorge Pombo-Otero
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  6. María Fraga-Mariño
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  7. Jacinto Sanchez-Ibañez
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  8. Nieves Doménech
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  9. María Esther Rendal-Vázquez
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Corresponding authors

Correspondence to Nieves Doménech or María Esther Rendal-Vázquez.

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Sanluis-Verdes, A., Sanluis-Verdes, N., Manso-Revilla, M.J. et al. Tissue engineering for neurodegenerative diseases using human amniotic membrane and umbilical cord. Cell Tissue Bank 18, 1–15 (2017). https://doi.org/10.1007/s10561-016-9595-0

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  • DOI: https://doi.org/10.1007/s10561-016-9595-0

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