Tissue reaction of deproteinized bovine bone matrix grafting in ectopic site: histological study on sheep
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The aim of this paper was to evaluate through histological analysis of the tissue reaction of deproteinized bovine bone matrix (DBBM) when inserted into the site of intramuscular ectopic sheep. In this study, 16 sheep received 3 groups and these were divided into 2 experimental times: Group 1—sham group, Group 2—particulate autogenous bone and Group 3—DBBM granules. All animals underwent surgical procedures for insertion of materials in an ectopic site (muscles of the lower back and after 3 and 6 months postoperatively, the samples were evaluated by histological analysis. The results indicated that the Sham group showed dense collagen fibers and thin, characterizing fibrosis at 3 and 6 months. In the autograft group there was a significant amount of collagen deposition and decreased inflammation at 6 months postoperatively. Group of DBBM, it was noted the presence of dense connective tissue and surrounding remaining particles was observed the formation of with osteoid characteristic tissue. The DBBM demonstrated biocompatibility, osteoconductivity and small osteogenesis capacity on ectopic site.
KeywordsBone grafts Reactions graft Bone regeneration Ectopic sites Deproteinized bovine bone matrix Sheep
Compliance with ethical standards
Human and animals rights statement
We the authors, also declare that this study was submitted to the Research Ethics Committee on Animal Use (CEUA) at the Positivo University (Protocol 21/2011) in accordance with the provisions of the Arouca Law (11794/2008) and ethical principles of the Brazilian Society of Laboratory Animal Science (SBCAL) and standard protocol for sedation in animals.
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