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Cell and Tissue Banking

, Volume 17, Issue 4, pp 643–651 | Cite as

Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane

  • Rusidah Mat Yatim
  • Thirumulu Ponnuraj KannanEmail author
  • Suzina Sheikh Ab Hamid
Article

Abstract

Human amniotic membrane (HAM) due to its high biocompatibility, low immunogenicity, anti-microbial, anti-viral properties as well as the presence of growth factors has been used in various clinical applications. The growth factors play an important role in wound healing. The current study aimed to explore the effect of 15 kGy gamma radiation dose on selected growth factors and receptors mRNA present in HAM. Eight growth factors, namely, EGF, HGF, KGF, TGF-α, TGF-β1, TGF-β2, TGF-β3 and bFGF and two growth factor receptors, HGFR and KGFR were evaluated in this study. The total RNA was extracted and converted to complimentary DNA using commercial kits. Subsequently, the mRNA expressions of these growth factors were evaluated using real-time PCR and the results were statistically analyzed using REST-MCS software. This study confirmed the presence of these mRNA growth factors and receptors in fresh, glycerol cryopreserved and irradiated glycerol cryopreserved HAM. In glycerol cryopreserved HAM, the results showed up-regulation of HGF and bFGF and down-regulation of EGF, HGFR, KGF, KGFR, TGF-α, TGF-β1, TGF-β2 and TGF-β3 relative to the fresh HAM which acted as the control, whereas in irradiated glycerol cryopreserved HAM, the results showed up-regulation of EGF, HGF, KGF, KGFR, TGF-β1, TGF-β2 and TGF-β3 and down-regulation of HGFR, TGF-α and bFGF relative to the glycerol cryopreserved HAM which acted as the control. However, these mRNA expressions did not show any statistical significant difference compared to the control groups. This study concluded that a dose of 15 kGy of gamma radiation did not affect the mRNA expression for the growth factors’ and receptors’ in the glycerol cryopreserved HAM.

Keywords

Human amniotic membrane Growth factors Gamma radiation Glycerol cryopreservation Gene expression 

Notes

Acknowledgments

The authors acknowledge the staff of USM Tissue Bank, Human Genome Centre and Department of Haematology of School of Medical Sciences and Laboratory of Forensic Science of School of Health Sciences for the technical support and USM Short Term Grant (304/PPSP/61311051), Malaysian Technology Development Corporation (MTDC Grant 304/PPSP/6150093.M130) and International Atomic Energy Agency (IAEA Research Contract No: 16099/RO) for the financial support to conduct this research.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10561_2016_9576_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 35 kb)

References

  1. Ab Hamid SS, Zahari NK, Yusof N, Hassan A (2014) Scanning electron microscopic assessment on surface morphology of preserved human amniotic membrane after gamma sterilisation. Cell Tissue Bank 15:15–24CrossRefPubMedGoogle Scholar
  2. Aghayan HR, Goodarzi P, Baradaran-Rafii A, Larijani B, Moradabadi L, Rahim F, Arjmand B (2013) Bacterial contamination of amniotic membrane in a tissue bank from Iran. Cell Tissue Bank 14:401–406CrossRefPubMedGoogle Scholar
  3. Atique FB, Ahmed KT, Asaduzzaman S, Hasan KN (2013) Effects of gamma irradiation on bacterial microflora associated with human amniotic membrane. BioMed Res Int 2013:1–6Google Scholar
  4. Baker TF, Ronholdt CJ, Bogdansky S (2005) Validating a low dose gamma irradiation process for sterilizing allografts using ISO 11137 method 2B. Cell Tissue Bank 6:271–275CrossRefPubMedGoogle Scholar
  5. Baradaran-Rafii A, Aghayan H-R, Arjmand B, Javadi M-A (2008) Amniotic membrane transplantation. J Ophthalmic Vis Res 2:58–75Google Scholar
  6. Bomfim Pereira MG, Pereira Gomes JA, Rizzo LV, Cristovam PC, Silveira LC (2016) Cytokine dosage in fresh and preserved human amniotic membrane. Cornea 35:89–94CrossRefPubMedGoogle Scholar
  7. Chakrabarti MC, Le N, Paik CH, De Graff WG, Carrasquillo JA (1996) Prevention of radiolysis of monoclonal antibody during labeling. J Nucl Med 37:1384–1388PubMedGoogle Scholar
  8. Deolinda de Oliveira Pena J, Melo GB, Gomes JA, Haapalainen EF, Komagome CM, Santos NC, Souza Lima Filho AA, Rizzo LV  (2007) Ultrastructural and growth factor analysis of amniotic membrane preserved by different methods for ocular surgery. Arq Bras Oftalmol 70:756–762CrossRefPubMedGoogle Scholar
  9. Dua HS, Gomes JA, King AJ, Maharajan VS (2004) The amniotic membrane in ophthalmology. Surv Ophthalmol 49:51–77CrossRefPubMedGoogle Scholar
  10. Dziedzic-Goclawska A, Kaminski A, Uhrynowska-Tyszkiewicz I, Stachowicz W (2005) Irradiation as a safety procedure in tissue banking. Cell Tissue Bank 6:201–219CrossRefPubMedGoogle Scholar
  11. Grzywocz Z et al (2014) Growth factors and their receptors derived from human amniotic cells in vitro. Folia Histochem Cytobiol 52:163–170CrossRefPubMedGoogle Scholar
  12. Han SK, Song JY, Yun YS, Yi SY (2006) Effect of gamma radiation on cytokine expression and cytokine-receptor mediated STAT activation. Int J Radiat Biol 82:686–697CrossRefPubMedGoogle Scholar
  13. Hennerbichler S, Reichl B, Pleiner D, Gabriel C, Eibl J, Redl H (2007) The influence of various storage conditions on cell viability in amniotic membrane. Cell Tissue Bank 8:1–8CrossRefPubMedGoogle Scholar
  14. Hilmy N, Febrida A, Basril A (2000) Validation of radiation sterilization dose for lyophilized amnion and bone grafts. Cell Tissue Bank 1:143–148CrossRefPubMedGoogle Scholar
  15. Hopkinson A, McIntosh RS, Tighe PJ, James DK, Dua HS (2006) Amniotic membrane for ocular surface reconstruction: donor variations and the effect of handling on TGF-beta content. Invest Ophthalmol Vis Sci 47:4316–4322CrossRefPubMedGoogle Scholar
  16. Iijima K, Igawa Y, Imamura T, Moriizumi T, Nikaido T, Konishi I, Nishizawa O (2007) Transplantation of preserved human amniotic membrane for bladder augmentation in rats. Tissue Eng 13:513–524CrossRefPubMedGoogle Scholar
  17. Kar IB, Singh AK, Mohapatra PC, Mohanty PK, Misra S (2014) Repair of oral mucosal defects with cryopreserved human amniotic membrane grafts: prospective clinical study. Int J Oral Maxillofac Surg 43:1339–1344CrossRefPubMedGoogle Scholar
  18. Kesting MR, Wolff KD, Nobis CP, Rohleder NH (2014) Amniotic membrane in oral and maxillofacial surgery. Oral Maxillofac Surg 18:153–164CrossRefPubMedGoogle Scholar
  19. Koizumi N, Inatomi T, Sotozono C, Fullwood NJ, Quantock AJ, Kinoshita S (2000) Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res 20:173–177CrossRefPubMedGoogle Scholar
  20. Kruse FE, Rohrschneider K, Völcker HE (1999) Multilayer amniotic membrane transplantation for reconstruction of deep corneal ulcers. Ophthalmology 106:1504–1511CrossRefPubMedGoogle Scholar
  21. Kruse F, Joussen A, Rohrschneider K, You L, Sinn B, Baumann J, Völcker H (2000) Cryopreserved human amniotic membrane for ocular surface reconstruction. Graefes Arch Clin Exp Ophthalmol 238:68–75CrossRefPubMedGoogle Scholar
  22. Kubo M, Sonoda Y, Muramatsu R, Usui M (2001) Immunogenicity of human amniotic membrane in experimental xenotransplantation. Invest Ophthalmol Vis Sci 42:1539–1546PubMedGoogle Scholar
  23. Laurent R, Nallet A, Obert L, Nicod L, Gindraux F (2014) Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank 15:267–275CrossRefPubMedGoogle Scholar
  24. Lee SB, Li DQ, Tan DT, Meller DC, Tseng SC (2000) Suppression of TGF-beta signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res 20:325–334CrossRefPubMedGoogle Scholar
  25. Litwiniuk M, Grzela T (2014) Amniotic membrane: new concepts for an old dressing. Wound Repair Regen 22:451–456CrossRefPubMedGoogle Scholar
  26. Lo V, Pope E (2009) Amniotic membrane use in dermatology. Int J Dermatol 48:935–940CrossRefPubMedGoogle Scholar
  27. Lopez-Valladares MJ, Teresa Rodriguez-Ares M, Tourino R, Gude F, Teresa Silva M, Couceiro J (2010) Donor age and gestational age influence on growth factor levels in human amniotic membrane. Acta Ophthalmol 88:e211–e216CrossRefPubMedGoogle Scholar
  28. Madhavan HN, Priya K, Malathi J, Joseph PR (2002) Preparation of amniotic membrane for ocular surface reconstruction. Indian J Ophthalmol 50:227PubMedGoogle Scholar
  29. Mamede A, Carvalho M, Abrantes A, Laranjo M, Maia C, Botelho M (2012) Amniotic membrane: from structure and functions to clinical applications. Cell Tissue Res 349:447–458CrossRefPubMedGoogle Scholar
  30. Mohammadi AA, Seyed Jafari SM, Kiasat M, Tavakkolian AR, Imani MT, Ayaz M, Tolide-ie HR (2013) Effect of fresh human amniotic membrane dressing on graft take in patients with chronic burn wounds compared with conventional methods. Burns 39:349–353CrossRefPubMedGoogle Scholar
  31. Nguyen H, Morgan DA, Forwood MR (2007a) Sterilization of allograft bone: effects of gamma irradiation on allograft biology and biomechanics. Cell Tissue Bank 8:93–105CrossRefPubMedGoogle Scholar
  32. Nguyen H, Morgan DA, Forwood MR (2007b) Sterilization of allograft bone: is 25 kGy the gold standard for gamma irradiation? Cell Tissue Bank 8:81–91CrossRefPubMedGoogle Scholar
  33. Nguyen H, Cassady AI, Bennett MB, Gineyts E, Wu A, Morgan DA, Forwood MR (2013) Reducing the radiation sterilization dose improves mechanical and biological quality while retaining sterility assurance levels of bone allografts. Bone 57:194–200CrossRefPubMedGoogle Scholar
  34. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM (2008) Properties of the amniotic membrane for potential use in tissue engineering. Eur Cells Mater 15:88–99Google Scholar
  35. Pegg DE (2007) Principles of cryopreservation. Methods Mol Biol 368:39–57CrossRefPubMedGoogle Scholar
  36. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36CrossRefPubMedPubMedCentralGoogle Scholar
  37. Phillips GO (2013) Jorge Morales Pedraza: the use of the ionization technique for tissue sterilisation: the International Atomic Energy Agency (IAEA) experience. Cell Tissue Bank 14:341–348CrossRefPubMedGoogle Scholar
  38. Qureshi IZ, Fareeha A, Khan WA (2010) Technique for processing and preservation of human amniotic membrane for ocular surface reconstruction. WASET 45:757–760Google Scholar
  39. Rahman I, Said DG, Maharajan VS, Dua HS (2009) Amniotic membrane in ophthalmology: indications and limitations. Eye (Lond) 23:1954–1961CrossRefGoogle Scholar
  40. Rama P, Giannini R, Bruni A, Gatto C, Tiso R, Ponzin D (2001) Further evaluation of amniotic membrane banking for transplantation in ocular surface diseases. Cell Tissue Bank 2:155–163CrossRefPubMedGoogle Scholar
  41. Rejzek A, Weyer F, Eichberger R, Gebhart W (2001) Physical changes of amniotic membranes through glycerolization for the use as an epidermal substitute. Light and electron microscopic studies. Cell Tissue Bank 2:95–102CrossRefPubMedGoogle Scholar
  42. Riau AK, Beuerman RW, Lim LS, Mehta JS (2010) Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction. Biomaterials 31:216–225CrossRefPubMedGoogle Scholar
  43. Rodriguez-Ares MT, Lopez-Valladares MJ, Tourino R, Vieites B, Gude F, Silva MT, Couceiro J (2009) Effects of lyophilization on human amniotic membrane. Acta Ophthalmol 87:396–403CrossRefPubMedGoogle Scholar
  44. Rooney P, Eagle M, Hogg P, Lomas R, Kearney J (2008) Sterilisation of skin allograft with gamma irradiation. Burns 34:664–673CrossRefPubMedGoogle Scholar
  45. Sarwar I, Sultana R, Nisa RU, Qayyum I (2010) Vaginoplasty by using amnion graft in patients of vaginal agenesis associated with Mayor–Rokitansky–Kuster–Hauser syndrome. J Ayub Med Coll Abbottabad 22:7–10PubMedGoogle Scholar
  46. Shortt AJ, Secker GA, Lomas RJ, Wilshaw SP, Kearney JN, Tuft SJ, Daniels JT (2009) The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex vivo expansion of limbal epithelial cells. Biomaterials 30:1056–1065CrossRefPubMedGoogle Scholar
  47. Silindir M, Ozer A (2009) Sterilization methods and the comparison of e-beam sterilization with gamma radiation sterilization. FABAD J Pharm Sci 34:43–53Google Scholar
  48. Singh R, Gupta P, Purohit S, Kumar P, Vaijapurkar SG, Chacharkar MP (2006) Radiation Resistance of the Microflora Associated with Amniotic Membranes. World J Microbiol Biotechnol 22:23–27CrossRefGoogle Scholar
  49. Tandon A, Tovey JCK, Sharma A, Gupta R, Mohan RR (2010) Role of transforming growth factor beta in corneal function, biology and pathology. Curr Mol Med 10:565–578PubMedPubMedCentralGoogle Scholar
  50. Thomasen H, Pauklin M, Steuhl K-P, Meller D (2009) Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications. Graefe’s Arch Clin Exp Ophthalmol 247:1691–1700CrossRefGoogle Scholar
  51. Thomasen H et al (2011) The effect of long-term storage on the biological and histological properties of cryopreserved amniotic membrane. Curr Eye Res 36:247–255CrossRefPubMedGoogle Scholar
  52. Uçakhan ÖÖ, Köklü G, Firat E (2002) Nonpreserved human amniotic membrane transplantation in acute and chronic chemical eye injuries. Cornea 21:169–172CrossRefPubMedGoogle Scholar
  53. von Versen-Hoeynck F, Steinfeld AP, Becker J, Hermel M, Rath W, Hesselbarth U (2008) Sterilization and preservation influence the biophysical properties of human amnion grafts. Biologicals 36:248–255CrossRefGoogle Scholar
  54. von Versen-Höynck F, Hesselbarth U, Möller D (2004a) Application of sterilised human amnion for reconstruction of the ocular surface. Cell Tissue Bank 5:57–65CrossRefGoogle Scholar
  55. von Versen-Höynck F, Syring C, Bachmann S, Möller D (2004b) The influence of different preservation and sterilisation steps on the histological properties of amnion allografts-light and scanning electron microscopic studies. Cell Tissue Bank 5:45–56CrossRefGoogle Scholar
  56. Wang S, Zinderman C, Wise R, Braun M (2007) Infections and human tissue transplants: review of FDA MedWatch reports 2001–2004. Cell Tissue Bank 8:211–219CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Rusidah Mat Yatim
    • 1
  • Thirumulu Ponnuraj Kannan
    • 2
    • 3
    Email author
  • Suzina Sheikh Ab Hamid
    • 1
  1. 1.Tissue Bank, School of Medical SciencesUniversiti Sains MalaysiaKubang KerianMalaysia
  2. 2.School of Dental SciencesUniversiti Sains MalaysiaKubang KerianMalaysia
  3. 3.Human Genome Centre, School of Medical SciencesUniversiti Sains MalaysiaKubang KerianMalaysia

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