Cell and Tissue Banking

, Volume 17, Issue 4, pp 543–553 | Cite as

Deceased tissue donor serology and molecular testing for HIV, hepatitis B and hepatitis C viruses: a lack of cadaveric validated tests

  • Thayssa Neiva da Fonseca Victer
  • Cris Stéphany Rodrigues dos Santos
  • Sônia Nair Báo
  • Thatiane Lima SampaioEmail author
Full Length Review


Vital to patient safety is the accurate assessment and minimization of risk for human immunodeficiency virus (HIV), Hepatitis C (HCV), and Hepatitis B (HBV) virus transmission by deceased donor organ and tissue transplantation. The pathogens are tested by serological kits based on enzyme-linked immunosorbent assay (ELISA), chemiluminescence (CLIA) and eletrochemiluminescence (ECLIA) immunoassays. Organ transplantation is a highly successful life-saving treatment in Brazil, but the Brazilian Health Surveillance Agency currently mandates that all deceased organ donors are screened for HIV, HCV and HBV following living donor policies. In this review, six ELISA (Wama®, Bio-Rad®, Biomerieux®, DiaSorin®, Acon Biotech® and Biokit®), three CLIA (Abbott®, Siemens®, Diasorin®) and one ECLIA (Roche®) were utilized for evaluating the effectiveness of those serological tests for deceased donors in Brazil according to manufacturer’s guidelines. NAT for HIV, HCV and HBV can assist with detection of pre-seroconversion for those infections, and only Cobas® TaqScreen MPX® test, the Tigris System® Procleix Ultrio Assay® and the Bio-Manguinhos® HIV/HCV/HBV NAT are commercially available. Between all the tests, only the manufacturer Abbott® and Cobas® TaqScreen MPX® test are currently validated for cadaver samples.


Post-mortem blood Donors Validation Infectious disease serology NAT Infectious disease molecular detection 



Antibody to hepatitis B virus core antigen


Hepatitis C virus antibody

Anti-HIV 1/2

Human immunodeficiency 1 and 2 virus antibody


Brazilian Health Surveillance Agency


Chemiluminescent assay


Eletrochemiluminescent assay


Enzyme linked immuno sorbent assay


Hepatitis B virus surface antigen


Nucleic acid test


Polymerase chain reaction



This work was supported by National Agency for Health Surveillance (ANVISA)/National Counsel for Scientific and Technological Development (CNPq) (Grants 403360/2012-9 and 440029/2014-7) and Foundation for Research Support of Distrito Federal (Grant 23098.021477.2015-87). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Language help was supported by Prime Scientífica LTDA-ME.

Compliance with ethical standards

Conflict of interest

The authors of this manuscript have no conflicts of interest to disclose.


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Thayssa Neiva da Fonseca Victer
    • 1
  • Cris Stéphany Rodrigues dos Santos
    • 1
  • Sônia Nair Báo
    • 1
  • Thatiane Lima Sampaio
    • 1
    • 2
    Email author
  1. 1.Department of Cell BiologyUniversity of BrasíliaBrasíliaBrazil
  2. 2.Federal Institute of BrasíliaBrasíliaBrazil

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