Abstract
Healthcare-associated pneumonia (HCAP) represents a major diagnostic challenge because of the relatively low sensitivity and specificity of clinical criteria, radiological findings, and microbiologic culture results. It is often difficult to distinguish between pneumonia, underlying pulmonary disease, or conditions with pulmonary complications; this is compounded by the often-subjective clinical diagnosis of pneumonia. We conducted this study to determine the utility of post-mortem lung biopsies for diagnosing pneumonia in tissue donors diagnosed with pneumonia prior to death. Subjects were deceased patients who had been hospitalized at death and diagnosed with pneumonia. Post-mortem lung biopsies were obtained from the anatomic portion of the cadaveric lung corresponding to chest radiograph abnormalities. Specimens were fixed, stained with hematoxylin and eosin, and read by a single board-certified pathologist. Histological criteria for acute pneumonia included intense neutrophilic infiltration, fibrinous exudates, cellular debris, necrosis, or bacteria in the interstitium and intra-alveolar spaces. Of 143 subjects with a diagnosis of pneumonia at time of death, 14 (9.8 %) had histological evidence consistent with acute pneumonia. The most common histological diagnoses were emphysema (53 %), interstitial fibrosis (40 %), chronic atelectasis (36 %), acute and chronic passive congestion consistent with underlying cardiomyopathy (25 %), fibro-bullous disease (12 %), and acute bronchitis (11 %). HCAP represents a major diagnostic challenge because of the relatively low sensitivity and specificity of clinical criteria, radiological findings, and microbiologic testing. We found that attending physician-diagnosed pneumonia did not correlate with post-mortem pathological diagnosis. We conclude that histological examination of cadaveric lung tissue biopsies enables ascertainment or rule out of underlying pneumonia and prevents erroneous donor deferrals.
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Notes
“Approximate” because some potential donors were rejected by the screening process before the cases came to the attention of the Medical Director of RTIS.
Loose definition CXR infiltrate (unilobar, unilateral, or bilateral) with two of the following three findings: temperature >38 °C or <35.5 °C; white blood cell count >10,000 cells/μL or <4000 cells/μL; or purulent respiratory secretions.Rigorous definition CXR infiltrate (unilobar, unilateral, or bilateral) with all of the following three findings: temperature >38 °C or <35.5 °C; white blood cell count >10,000 cells/μL or <4000 cells/μL; purulent respiratory secretions.
References
American Association of Tissue Banks (2012) Standards for tissue banking, 13th edn. American Association of Tissue Banks, McLean
American Thoracic Society; Infectious Diseases Society of America (2005) Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare- associated pneumonia. Am J Respir Crit Care Med 171(4):388–416
Boyce JM, Potter-Bynoe G, Dziobek L, Solomon SL (1991) Nosocomial pneumonia in Medicare patients. Hospital costs and reimbursement patterns under the prospective payment system. Arch Int Med 151(6):1109–1114
Diebold J (2007) Clinical autopsy—its role in modern medicine. Prax 96(43):1667–1671
Eisenhuber E, Schaefer-Prokop CM, Prosch H, Schima W (2012) Bedside chest radiography. Respir Care 57(3):427–443
Food and Drug Administration (2013) Code of federal regulations—food and drugs. Part 1271: human cells, tissues, and cellular and tissue-based products. In: Administration FaD (ed) Title 21, vol 8. Washington, DC. Government Printing Office; Revised as of 1 April 2013
Kallinen O, Partanen TA, Maisniemi K, Bohling T, Tukiainen E, Koljonen V (2008) Comparison of premortem clinical diagnosis and autopsy findings in patients with burns. Burns 34(5):595–602
Klompas M (2013) Complications of mechanical ventilation—The CDC’s new surveillance paradigm. N Engl J Med 368:1472–1475
Klompas M, Kleinman K, Khan Y et al (2012) Rapid and reproducible surveillance for ventilator-associated pneumonia. Clin Infect Dis 54(3):370–377
Light TD, Royer NA, Zabell J et al (2011) Autopsy after traumatic death—a shifting paradigm. J Surg Res 167(1):121–124
Loeb MB, Carusone SB, Marrie TJ et al (2006) Interobserver reliability of radiologists’ interpretations of mobile chest radiographs for nursing home-acquired pneumonia. J Am Med Dir Assoc 7(7):416–419
Meade MO, Cook RJ, Guyatt GH et al (2000) Interobserver variation in interpreting chest radiographs for the diagnosis of acute respiratory distress syndrome. Am J Respir Crit Care Med 161(1):85–90
Meduri GU, Mauldin GL, Wunderink RG et al (1994) Causes of fever and pulmonary densities in patients with clinical manifestations of ventilator-associated pneumonia. Chest 106(1):221–235
Nemetz PN, Tanglos E, Sands LP, Fisher WP Jr, Newman WP 3rd, Burton EC (2006) Attitudes toward the autopsy—an 8-state survey. Med Gen Med 8(3):80
Rea-Neto A, Youssef NC, Tuche F et al (2008) Diagnosis of ventilator-associated pneumonia: a systematic review of the literature. Crit Care 12(2):R56
Richards MJ, Edwards JR, Culver DH, Gaynes RP (1999) Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Crit Care Med 27(5):887–892
Robbins SL, Angell M (1976) Respiratory system. Basic pathology, 2nd edn. WB Saunders Company, Philadelphia, pp 390–391
Schurink CA, Van Nieuwenhoven CA, Jacobs JA et al (2004) Clinical pulmonary infection score for ventilator-associated pneumonia: accuracy and inter-observer variability. Intensive Care Med 30(2):217–224
Shojania KG, Burton EC, McDonald KM, Goldman L (2003) Changes in rates of autopsy-detected diagnostic errors over time: a systematic review. JAMA 289(21):2849–2856
Sievert DM, Ricks P, Edwards JR et al (2013) Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009–2010. Infect Control Hosp Epidemiol 34(1):1–14
Tejerina E, Esteban A, Fernandez-Segoviano P et al (2010) Accuracy of clinical definitions of ventilator-associated pneumonia: comparison with autopsy findings. J Crit Care 25(1):62–68
Tejerina E, Esteban A, Fernandez-Segoviano P et al (2012) Clinical diagnoses and autopsy findings: discrepancies in critically ill patients. Crit Care Med 40(3):842–846
Thomas CP, Ryan M, Chapman JD et al (2012) Incidence and cost of pneumonia in medicare beneficiaries. Chest 142:973–981
Wunderink RG, Woldenberg LS, Zeiss J, Day CM, Ciemins J, Lacher DA (1992) The radiologic diagnosis of autopsy-proven ventilator-associated pneumonia. Chest 101(2):458–463
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Herman Baer: Died in June 2014.
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Kubilay, Z., Layon, A.J., Baer, H. et al. When is pneumonia not pneumonia: a clinicopathologic study of the utility of lung tissue biopsies in determining the suitability of cadaveric tissue for donation. Cell Tissue Bank 17, 205–210 (2016). https://doi.org/10.1007/s10561-016-9545-x
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DOI: https://doi.org/10.1007/s10561-016-9545-x