Cell and Tissue Banking

, Volume 14, Issue 3, pp 465–474 | Cite as

Development of a decellularised dermis

  • Penny HoggEmail author
  • Paul Rooney
  • Eileen Ingham
  • John N. Kearney
Original Paper


The purpose of this investigation was to develop a decellularised human dermis suitable for allografting. Samples of human skin were obtained from deceased donors and taken through a series of steps to remove all cellular material. The steps were: chemical removal of the epidermis, disinfection, lysing of cells in hypotonic buffer, a detergent treatment and a nuclease buffer to remove residual nuclear material. Histological preparations of the decellularised dermis produced were then investigated. In addition residual DNA content, structural strength, collagen denaturation, cytotoxicity and in vivo tissue reactivity following implantation in a murine model were examined. For all donors tested there was no change in morphology as viewed by light microscopy. Mean DNA removal was evaluated at 92.1 %. There were no significant changes in structural strength or evidence of collagen degradation. The tissue did not appear to be cytotoxic or elicit an immune response when implanted in the mouse model. A decellularised tissue has been developed that would appear to be suitable for a range of surgical procedures.


Dermis Decellularised Skin Allograft 


  1. Ahmad S, Kolli S, Lako M, Figueiredo F, Daniels JT (2010) Stem cell therapies for ocular surface disease. Drug Discov Today 15:306–313PubMedCrossRefGoogle Scholar
  2. Badylak SF (2008) Naturally occurring scaffold materials. In: Atala A, Lanza R, Thomson J, Nerem R (eds) Principals of regenerative medicine. Academic Press, Waltham, pp 594–603CrossRefGoogle Scholar
  3. Booth C, Korossis S, Wilcox HE, Watterson KG, Kearney JN, Fisher J, Ingham E (2002) Tissue engineering a cardiac valve prosthesis I: development and histological characterisation of an acellular porcine scaffold. J Heart Valve Dis 11:457–462PubMedGoogle Scholar
  4. Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularisation processes. Biomaterials 32:3233–3243PubMedCrossRefGoogle Scholar
  5. da Costa FD, Santos LR, Collatusso C, Matsuda CN, Lopes SA, Carduro S, Roderjan JG, Ingham E (2009) Thirteen years’ experience of the Ross operation. J Heart Valve Dis 18:84–94PubMedGoogle Scholar
  6. Gilbert WT, Sellaro TL, Badylak SF (2006) Decellularisation of tissues and organs. Biomaterials 27:3675–3683PubMedGoogle Scholar
  7. Korossis S, Booth C, Wilcox HE, Ingham E, Kearney JN, Watterson K, Fisher J (2002) Tissue engineering a cardiac valve prosthesis II: biomechanical characterisation of decellularised porcine heart valves. J Heart Valve diseases 11:463–471Google Scholar
  8. Macchiarini P, Jungebluth P, Go T, Asnaghi MA, Rees LE, Cogan TA, Dodson A, Martorell J, Bellini S, Parnigotto PP, Dickinson SC, Hollander AP, Mantero S, Conconi MT, Birchall MA (2008) Clinical transplantation of a tissue-engineered airway. Lancet 372:2023–2030PubMedCrossRefGoogle Scholar
  9. Mirsadraee S, Wilcox HE, Korossis S, Kearney JN, Watterson KG, Fisher J, Ingham E (2006) Tissue engineering of pericardium: development and characterisation of an acellular human pericardial matrix. Tissue Eng 12:763–773PubMedCrossRefGoogle Scholar
  10. Navarro FB, da Costa DA, Mulinari LA, Pimental GK, Roderjan JG, Vieira ED, de Noronha L, Miyague NI (2010) Evaluation of the biological behaviour of decellularised pulmonary homografts: an experimental sheep model. Rev Bras Cardiovasc 35:377–387CrossRefGoogle Scholar
  11. Sheridan RL, Choucair RJ (1997) Acellular allogenic dermis does not hinder initial engraftment in burn wound resurfacing and reconstruction. Burn Care Rehabil 18:496–499CrossRefGoogle Scholar
  12. Stapleton TW, Ingram J, Fisher J, Ingham E (2011) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications. Tissue Eng (A) 17:231–242CrossRefGoogle Scholar
  13. Svevchenko RV, Jones SL, James SE (2010) A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface 7:229–258CrossRefGoogle Scholar
  14. Tischer T, Vogt S, Aryee S, Steinhauser E, Adamczyk C, Milz S, Martinek V, Imhoff AB (2007) Tissue engineering of the anterior cruciate ligament: a new method using acellularized tendon allografts and autologous fibroblasts. Arch Orthop Trauma Surg 127:735–741PubMedCrossRefGoogle Scholar
  15. Wilcox HE, Korossis SA, Booth C, Watterson KG, Kearney JN, Fisher J, Ingham E (2005) Biocompatability and recelularisation potential of an acellular porcine heart valve matrix. J Heart Valve Dis 14:228–236PubMedGoogle Scholar
  16. Wilshaw SP, Kearney JN, Fisher J, Ingham E (2006) Production of an acellular amniotic membrane matrix for use in tissue engineering. Tissue Eng 12:2117–2129Google Scholar
  17. Wilshaw SP, Kearney J, Fisher J, Ingham E (2008) Biocompatibility and potential of acellular human amniotic membrane to support the attachment and proliferation of allogeneic cells. Tissue Eng (A) 14:463–472CrossRefGoogle Scholar
  18. Wilshaw SP, Rooney P, Berry H, Kearney JN, Homer-Vanniasinkam S, Fisher J, Ingham E (2012) Development and characterisation of acellular allogeneic arterial matrices. Tissue Eng (A) 18:471–483Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Penny Hogg
    • 1
    Email author
  • Paul Rooney
    • 1
  • Eileen Ingham
    • 2
  • John N. Kearney
    • 1
  1. 1.NHS Blood and Transplant, Tissue Services R&DLiverpoolUK
  2. 2.IMBE, University of LeedsLeedsUK

Personalised recommendations