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Effect of two cleaning processes for bone allografts on gentamicin impregnation and in vitro antibiotic release

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Abstract

Bone allografts are a useful and sometimes indispensable tool for the surgeon to repair bone defects. Microbial contamination is a major reason for discarding allografts from bone banks. To improve the number of safe allografts, we suggest chemical cleaning of the grafts followed by antibiotic impregnation. Comparison of two chemical cleaning processes for bone allografts aiming for antibiotic impregnation and consequently delivery rates in vitro. Bone chips of 5–10 mm were prepared from human femoral heads. Two cleaning methods (cleaning A and cleaning B) based on solutions containing hydrogen peroxide, paracetic acid, ethanol and biological detergent were carried out and compared. After the cleaning processes, the bone chips were impregnated with gentamicin. Bacillus subtilis bioassay was used to determine the gentamicin release after intervals of 1–7 days. Differences were compared with non-parametric Mann–Whitney U tests. The zones of inhibition obtained from the bone grafts cleaned with both cleaning processes were similar between the groups. The concentration of the released antibiotic was decreasing gradually over time, following a similar pattern for both groups. The cleaning procedure A as well as the cleaning procedure B for bone allografts allowed the impregnation with gentamicin powder in the same concentrations in both groups. The delivery of gentamicin was similar for both groups. Both cleaning procedures were easy to be carried out, making them suitable for routine use at the bone banks.

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Acknowledgments

We thank Dennis Huber for his technical support and for his help with statistics. We thank David Putzer for his help with data obtainment. The authors did not receive any payment or benefits from commercial institutions related directly or indirectly to the subject of this article. This study was sponsored by Experimental Orthopaedics, Medical University Innsbruck.

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The authors declare no conflict of interest.

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Correspondence to D. C. Coraça-Huber.

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Coraça-Huber, D.C., Hausdorfer, J., Fille, M. et al. Effect of two cleaning processes for bone allografts on gentamicin impregnation and in vitro antibiotic release. Cell Tissue Bank 14, 221–229 (2013). https://doi.org/10.1007/s10561-012-9314-4

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  • DOI: https://doi.org/10.1007/s10561-012-9314-4

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