The implementation of nucleic acid amplification technology testing for living tissue donors
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There is a significant requirement within the United Kingdom for tissue grafts from living donors. To ensure safety, blood samples from these donors are tested for pathogens at donation, and at 180 days post donation. Nucleic acid amplification technology (NAT) permits more sensitive detection of pathogens in blood samples than serum antigen testing. NAT testing can be applied to samples from living tissue donors to eliminate the need to re-test these donors 180 days post-donation before grafts can be implanted. This has major financial and operational advantages for a tissue bank, and this manuscript describes how NAT testing was assessed and implemented by NHSBT Tissue Services. When compared to traditional serum antigen testing, NAT testing was more cost effective, more convenient for donors and resulted in a greater proportion of donated grafts being made available for transplant.
KeywordsNucleic acid amplification technology (NAT) Tissue Services (TS) Femoral head Amniotic membrane Blood testing Donors
We would like to thank NHSBT Brentwood Testing Laboratory, especially Steven Tassen for all their hard work, which enabled NAT testing to be implemented with TS.
- Busch MP (2007) Evolving approaches to estimate risks of transfusion-transmitted viral infections: incidence-window period model after 10 years. In: Dax EM, Farrugia A, Vyas GN (eds) Advances in transfusion safety—volume IV, vol 127. Developments in Biologicals, Karger, pp 87–112Google Scholar
- Commission Directive (2006) Available via http://www.dh.gov.uk/en/Publichealth/Scientificdevelopmentgeneticsandbioethics/Tissue/Tissuegeneralinformation/DH . Accessed 23 March 2009
- James V (2005) Chapter 10 Microbiology tests for donors and donations: general specifications for laboratory test procedures. In: James V (ed) TSO, London Guidelines for the blood Transfusion Services in the United Kingdom, 7th edn, pp 137–141Google Scholar