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Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes

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Abstract

The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be also useful for other parts of the human body. Many researchers still use controversial methods; therefore it was evaluated and compared the efficiency of the enzymatic and direct explant methods to obtain oral keratinocytes. To this project oral epithelia fragments were used. This work compared: time needed for cell obtainment, best cell amount, life-span and epithelia forming cell capacity. The results showed the possibility to obtain keratinocytes from a small oral fragment and we could verify the advantages and peculiar restrictions. We concluded that under our conditions the enzymatic method showed the best results: in the cells obtaining time needed, cell amount and life-span. Both methods showed the same capacity to form in vitro epithelia.

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Authors and Affiliations

  1. Radiation Technology Center (CTR), Nuclear and Energetic Research Institute-IPEN/CNEN, Av. Lineu Prestes 2242, Cidade Universitária, São Paulo, Brazil

    Mª Fátima Guarizo Klingbeil, Daniele Yoshito & Monica Beatriz Mathor

  2. Medical School, Micro and Plastic Surgery Department, São Paulo University, Av. Dr. Arnaldo 455, Cerqueira César, 01246-903, São Paulo, SP, Brazil

    Marisa Roma Herson

  3. Mathematic and Statistic Institute, São Paulo University, Cidade Universitária Rua do Matão 1010, Cidade Universitária, 05508-000, São Paulo, SP, Brazil

    Elier Broche Cristo

  4. Dental School Pathology Department, São Paulo University, Av. Lineu Prestes 2227, Cidade Universitária, 05508-000, São Paulo, SP, Brazil

    Décio dos Santos Pinto Jr

Authors
  1. Mª Fátima Guarizo Klingbeil
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  2. Marisa Roma Herson
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  3. Elier Broche Cristo
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  4. Décio dos Santos Pinto Jr
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  5. Daniele Yoshito
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  6. Monica Beatriz Mathor
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Correspondence to Mª Fátima Guarizo Klingbeil.

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Klingbeil, M.F.G., Herson, M.R., Cristo, E.B. et al. Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes. Cell Tissue Bank 10, 197–204 (2009). https://doi.org/10.1007/s10561-009-9122-7

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  • DOI: https://doi.org/10.1007/s10561-009-9122-7

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