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Effects of freezing on bone histological morphology

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Abstract

Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue. Although this technique is considered safe, there is little information about the morphological modifications that the medullary and cortical portions of bone suffer after freezing. Hence, the aim of this study was to investigate the morphology of bone tissue after freezing under different temperatures and periods. Twelve rabbits were used to analyze the effects of two temperatures, −20°C and −70°C, during four periods of time: 30, 60, 90, 120 days. Tissues were analyzed by HE, picro-sirius stains and also by Feulgen’s reaction, through qualitative and morphometric ways, considering the area occupied by cells and nuclei, medullary and cortical portions, as well as by collagen expression at cortical. The differences among the treatments were analyzed by Tukey´s test, at 5% significance level. Bone freezing increased cellular and nuclear areas at cancellous bone and diminished nuclear area at the cortical bone. Cortical bone collagen suffered denaturation proportionally to temperature decrease and to freezing duration. These alterations compromised the morphology of tissues after 90 or 120 days of freezing at the temperature of −70°C. Cells necrosed during freezing, contributing to reduce bone antigenicity.

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Acknowledgement

The authors are grateful to FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia) for the financial support provided for this study.

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Authors and Affiliations

  1. Maxillofacial Surgery Division, Bahia Foundation for Science Development, Salvador, BA, Brazil

    Miguel Gustavo Setúbal Andrade & Antônio Márcio Teixeira Marchionni

  2. Post-graduation Program in Immunology, Bahia Federal University, Salvador, BA, Brazil

    Miguel Gustavo Setúbal Andrade, Thereza Cristina Bório dos Santos Calmon de Bittencourt & Moysés Sadigursky

  3. Division of Maxillofacial Surgery, Fundação Bahiana para o Desenvolvimento das Ciências, Avenida Silveira Martins, n. 3386. Cabula, Salvador, BA, Brazil, CEP 41150-100

    Miguel Gustavo Setúbal Andrade

  4. Tutorial Educational Program. School of Dentistry, Bahia Federal University, Salvador, BA, Brazil

    Camila Neves Sá

  5. Post-graduation Program in Laser in Dentirtry, Bahia Federal University, Salvador, BA, Brazil

    Antônio Márcio Teixeira Marchionni

  6. Veterinary School, Bahia Federal University, Salvador, BA, Brazil

    Thereza Cristina Bório dos Santos Calmon de Bittencourt

  7. Edgard Santos Hospital, Bahia Federal University, Salvador, BA, Brazil

    Moysés Sadigursky

Authors
  1. Miguel Gustavo Setúbal Andrade
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  2. Camila Neves Sá
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  3. Antônio Márcio Teixeira Marchionni
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  4. Thereza Cristina Bório dos Santos Calmon de Bittencourt
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Correspondence to Miguel Gustavo Setúbal Andrade.

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Andrade, M.G.S., Sá, C.N., Marchionni, A.M.T. et al. Effects of freezing on bone histological morphology. Cell Tissue Bank 9, 279–287 (2008). https://doi.org/10.1007/s10561-008-9065-4

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  • DOI: https://doi.org/10.1007/s10561-008-9065-4

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