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Influence of cryopreservation on human periodontal ligament cells in vitro

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Abstract

Cryopreservation of teeth before autotransplantation may create new possibilities in dentistry. The purpose of this study was to examine the effect of a standardised cryopreservation procedure on human periodontal ligament (PDL) cell cultures. Human PDL fibroblasts obtained from immature third molars of 11 patients were cultured and divided into two groups. The experimental group was cryopreserved and cultured after thawing. The control group was cultured without cryopreservation. A comparison was made between cryopreserved and control cells. To evaluate possible differences in the characteristics of the fibroblasts, the cells in both groups were tested for viability (membrane integrity), growth capacity and alkaline phosphatase (ALP) expression. The Wilcoxon test for paired comparison between cryopreserved and non-cryopreserved cells was performed for each characteristic. The results showed that membrane integrity of cells was not influenced by cryopreservation. There was no statistically significant difference in growth capacity between cryopreserved and control cells. Non-cryopreserved cells were slightly stronger positive for ALP, but the difference was not statistically significant. From these experiments it can be concluded that the observed parameters are not influenced by cryopreservation.

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Authors and Affiliations

  1. Department of Orthodontics, University Hospital of Ghent, De Pintelaan 185, Ghent, 9000, Belgium

    Liesbeth Temmerman, Luc R. Dermaut & Guy A. M. De Pauw

  2. Tissue bank, University Hospital of Ghent, Ghent, 9000, Belgium

    Martine De Mil & Hilde Beele

  3. Medical Informatics and Statistics, University Hospital of Ghent, Ghent, 9000, Belgium

    Georges Van Maele

Authors
  1. Liesbeth Temmerman
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  2. Luc R. Dermaut
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  3. Martine De Mil
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  4. Georges Van Maele
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  5. Hilde Beele
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  6. Guy A. M. De Pauw
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Correspondence to Liesbeth Temmerman.

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Temmerman, L., Dermaut, L.R., De Mil, M. et al. Influence of cryopreservation on human periodontal ligament cells in vitro. Cell Tissue Banking 9, 11–18 (2008). https://doi.org/10.1007/s10561-007-9047-y

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  • DOI: https://doi.org/10.1007/s10561-007-9047-y

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