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Effect of rat salivary glands extracts on the proliferation of cultured skin cells - a wound healing model

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Abstract

Objective: Salivary gland secretions play an important role in promotion of wound healing. The healing of intra- or extra-oral wounds is delayed in desalivated rats. However, the specific role of each salivary gland in promoting wound healing is unknown. This study was aimed to investigate the effect of crude extracts of rat salivary glands on a simplified in vitro wound healing model. Design/methods: Cultured human keratinocytes (HaCat) and murine fibroblasts (3T3) were subjected to 48 h serum starvation, and were later activated by extracts of rat salivary glands, 1–10 µg protein/ml of each gland. The resultant cellular metabolic activity of the activated cells was determined 24 h later, measuring reduction of XTT by mitochondrial enzymes, and calculated relatively to positive controls [optimal supplementation of 10% fetal calf serum (FCS)], and negative controls (starved non-supplemented cells). Results: The relative stimulatory effect of parotid (P) extract on the cells was significantly lower than either submandibular (SM) or sublingual (SL) extracts. Under the assumption that physiologically, the cells are exposed to the combined effect of saliva secreted from all the glands, different combinations of the extracts were presented to the cells. The relative stimulation was maximal following treatment with the three glands extracts (P + SM + SL) and exceeded the effect of 10% FCS. Conclusion: The results suggest that each salivary gland has a specific effect on wound healing and the combination of the three extracts has an additive effect but no the sum of all individual glands. This model might be useful to study the wound healing effect of salivary glands.

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Abbreviations

BSA:

Bovine serum albumin

ELISA:

Enzyme linked immunoassay

FCS:

Fetal calf serum

P:

Parotid salivary gland

SL:

Sublingual salivary gland

SM:

Submandibular salivary gland

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Authors and Affiliations

  1. Skin Bank and Investigative Dermatology Laboratory, Soroka University Medical Center, Beer-Sheva, Israel

    Nili Grossman & Li-At Binyamin

  2. Department of Microbiology and Immunology, Ben-Gurion Cancer Research Center, Beer-Sheva, Israel

    Nili Grossman

  3. Department of Oral and Maxillofacial Surgery, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel

    Lipa Bodner

Authors
  1. Nili Grossman
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  2. Li-At Binyamin
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  3. Lipa Bodner
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Correspondence to Lipa Bodner.

Additional information

In partial fulfillment of the requirement for MD thesis, The Joyce and Irving Goldman School of Medicine, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.

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Grossman, N., Binyamin, LA. & Bodner, L. Effect of rat salivary glands extracts on the proliferation of cultured skin cells - a wound healing model. Cell Tissue Banking 5, 205–212 (2004). https://doi.org/10.1007/s10561-005-4367-2

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  • DOI: https://doi.org/10.1007/s10561-005-4367-2

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