Abstract
Our previous work has shown that total osseous reconstruction of large discontinuity hemimandibulectomy, critical-sized defects can be achieved easily in 8-year-old Macaca fascicularis monkeys (Boyne 1996). However the literature has indicated that animal aging decreases the BMP induction of stem cells in rats and in other rodent species. It was necessarily important that the rhBMP-2 be demonstrated in non-human primates to determine if this reduction in effectiveness also existed in the higher animals phylogenetically. The purpose of this study was to operate aged non-human primates duplicating the model used in middle-aged animals to demonstrate regeneration of hemimandibulectomy defects. This age group could be extrapolated to the 80-year-old clinic patient. Six non-human primates aged 20 years were rendered edentulous posteriorly and the mandibles allowed to heal. Three months postoperatively bilateral hemimandibulectomies were performed. The defects received BMP in a collagen sponge (Helistat) using a dose level of 0.75 mg of rhBMP-2. After the manner previously reported by Boyne (1996, 1999), at the end of four months the surgical sites were exposed by mucoperiosteal flap demonstrating complete regeneration of the critical-sized defects. The animals received two dental implants in restored areas. The implants were brought into function approximately four months later, and were allowed to function for eight months in all cases. The results indicate that the regeneration of mandibular critical-sized defects by the use of rhBMP-2 in aged animals is comparable to that of the middle-aged group. This study indicates that aged non-human primates, chronologically comparable to 80-year-old humans, respond as favorably to rhBMP-2 as do the middle-aged animals. Extrapolating the results to the clinical level, one would expect that rhBMP-2 would produce a comparable result in the regeneration of large hemimandibulectomy-type defects in clinical human patients.
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References
Boyne P.J. 1996. Animal studies of the application of rhBMP-2 in Maxillofacial reconstruction. Bone (Suppl): 83S–92S.
P.J. Boyne A. Nakamura S. Shabahang (1999a) ArticleTitleEvaluation of the long-term effect of function on rhBMP-2 regenerated hemimandibulectomy defects Br. J. Oral Maxillofac. Surg. 37 344–352 Occurrence Handle10.1054/bjom.1999.0205 Occurrence Handle1:STN:280:DC%2BD3c%2FktlOjug%3D%3D
Boyne P.J., Salina S., Audia F. and Shabahang S. 1999b. Bone regeneration of rhBMP-2 induction in hemimandibulectomytype defecs of elderly monkeys. Cleveland, Ohio, Abstract. ACOMS Annual Scientific Conference, (Published in Br. J. OMS, December 1999).
J.C. Fleet K. Cashman K. Cox V. Rosen (1996) ArticleTitleThe effects of aging on the bone inductive activity of recombinant human bone morphogenetic protein-2 Endocrinology 137 4605–4610 Occurrence Handle10.1210/en.137.11.4605 Occurrence Handle1:CAS:528:DyaK28Xms1Cqtbc%3D Occurrence Handle8895323
K. Hoshi N. Amizuka T. Sakou T. Kurokawa H. Ozawa (1997) ArticleTitleFibroblasts of spinal ligaments pathologically differentiate into chondrocytes induced by recombinant human bone morphogenetic protein-2 Morphological examinations for ossification of spinal ligaments Bone 21 155–162 Occurrence Handle10.1016/S8756-3282(97)00106-3 Occurrence Handle1:CAS:528:DyaK2sXlvV2qtLw%3D Occurrence Handle9267691
K. Katagiri A. Yamaguchi M. Komaki et al. (1994) ArticleTitleBone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage J. Cell Biol. 127 1755–1766 Occurrence Handle10.1083/jcb.127.6.1755 Occurrence Handle1:CAS:528:DyaK2cXmvFemtLs%3D Occurrence Handle7798324
M. Komaki T. Katagiri T. Suda (1996) ArticleTitleBone morphogenetic protein-2 does not alter the differentiation pathway of committed progenitors of osteoblasts and chondroblasts Cell Tissue Res. 284 9–17 Occurrence Handle10.1007/s004410050562 Occurrence Handle1:CAS:528:DyaK28XivFWisr8%3D Occurrence Handle8601300
E. Marukawa I. Asahina M. Oda I. Seto M.I. Alam S. Enomoto (2001) ArticleTitleBone regeneration using recombinant human bone morphogenetic protein-2 (rhBMP-2) in alveolar defects of primate mandibles Br. J. Oral Maxillofac. Surg. 39 452–459 Occurrence Handle10.1054/bjom.2001.0673 Occurrence Handle1:STN:280:DC%2BD3MnptFWhsw%3D%3D Occurrence Handle11735142
S. Miyamoto K. Takaoka K. Ono (1993) ArticleTitleBone induction in monkeys by bone morphogenetic protein. A trans-filter technique J. Bone Joint Surg. 75 IssueIDB 107–110 Occurrence Handle1:STN:280:ByyC3s3gtFI%3D
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Boyne, P.J., Salina, S., Nakamura, A. et al. Bone Regeneration Using rhBMP-2 Induction in Hemimandibulectomy Type Defects of Elderly Sub-Human Primates. Cell Tissue Banking 7, 1–10 (2006). https://doi.org/10.1007/s10561-005-2242-9
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DOI: https://doi.org/10.1007/s10561-005-2242-9