Abstract
Introduction: An artificial complete skin (dermis and epidermis) model has been developed in the Tissue engineering unit of the Centro Comunitario de Sangre y Tejidos del Principado de Asturias (CCST) and CIEMAT. This engineered skin has been employed for the treatment of severe epithelial injuries. In this paper, the clinical results obtained with this engineered skin during the last 18 months were evaluated. Patients, material and methods: (a) Culture: Cells (fibroblasts and keratinocytes) were obtained from biopsies by a double enzymatic digestion. After an expansion period, fibroblasts were seeded in an artificial dermis based on human plasma. Keratinocytes were seeded over this dermal surface. (b) Patients: 20 skin biopsies were processed (13 burned patients, 5 giant nevus, 1 GVHD, 1 neurofibromatosis), which came from different hospitals across the country. About 97,525 cm2 of engineered skin were cultured. Results: The engineered skin took in all patients. The take percentage depended on previous pathology (burned patients 10–90%; non-critical patients 70–90%). The epithelization obtained was permanent in all cases. During the follow-up period, epithelial loss, blistering injuries or skin retractions were not observed. The aesthetic and functional results were acceptable. Conclusions: This artificial skin has demonstrated to be useful for the definitive treatment of patients with severe skin injuries. This work shows that it is possible to produce this prototype in an hospitalarian laboratory and distribute it to different hospitals across the country.
Similar content being viewed by others
References
E. Bell H.P. Ehrlich D.J. Buttle T. Nakatsuji (1981) ArticleTitleLiving tissue formed in vitro and accepted as skin equivalent tissue of full thickness Science 211 1052–1054 Occurrence Handle1:STN:280:Bi6C38njt1Y%3D Occurrence Handle7008197
E. Bell S. Sher B. Hull (1983) ArticleTitleThe reconstitution of living skin J. Invest. Dermatol. 81 2s–10s Occurrence Handle10.1111/1523-1747.ep12539993 Occurrence Handle1:STN:280:BiyB2c%2FgvFA%3D Occurrence Handle6306115
S.T. Boyce D.G. Greenhalgh R.J. Kagan T. Housinger J.M. Sorrell C.P. Childress M. Rieman G.D. Warden (1993) ArticleTitleSkin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers Plast. Teconstr. Surg. 91 632–641 Occurrence Handle1:STN:280:ByyC1cbns1Q%3D
M.L. Cooper C. Andree J.F. Hansbrough R.L. Zapata-Sirvent R.L. Spielvogel (1993) ArticleTitleDirect comparison of a cultured composite skin substitute containing human keratinocytes and fibroblasts to an epidermal sheet graft containing human keratinocytes on athymic mice J. Invest. Dermatol. 101 811–819 Occurrence Handle1:STN:280:ByuD28ritl0%3D Occurrence Handle8245510
M.L. Cooper J.F. Hansbrough R.L. Spielvogel R. Cohen R.L. Bartel G. Naughton (1991) ArticleTitleIn vivo optimisation of a living dermal substitute employing cultured human fibroblast on a biodegradable polyglycolic acid or polyglactin mesh Biomaterials 12 243–248 Occurrence Handle10.1016/0142-9612(91)90207-Q Occurrence Handle1:STN:280:By6A2cnjtFM%3D Occurrence Handle1652296
M.H. Desai J.M. Mlakar R.L. McCauley (1991) ArticleTitleLack of long-term durability of cultured keratinocyte burn-wound coverage: a case report J. Burn. Care Rehabil. 12 540–545 Occurrence Handle1:STN:280:By2C3sfntVQ%3D Occurrence Handle1779008
S.G. Llames M. Del Rio F. Larcher E. García M. García M. Jose Escamez J.L. Jorcano P. Holguín A. Meana (2004) ArticleTitleHuman plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin Trasnplantation 77 350–355
A. Meana J. Iglesias M. Del Rio F. Larcher S. Madrigal M.F. Fresno C. Martin F. San Roman F. Tevar (1998) ArticleTitleLarge surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels Burns 24 621–630 Occurrence Handle10.1016/S0305-4179(98)00107-7 Occurrence Handle1:STN:280:DyaK1M%2Fps1ertQ%3D%3D Occurrence Handle9882060
A. Meana J. Iglesias B. Madrigal J. Sánchez (1997) ArticleTitleUse of cyanoacrylate glue to prepare cultured keratinocyte sheets for grafting Burns 23 645–646 Occurrence Handle1:STN:280:DyaK1c3ivFOltg%3D%3D Occurrence Handle9568343
W.R. Otto J. Nanchahal Q.L. Lu N. Boddy R. Dover (1995) ArticleTitleSurvival of allogeneic cells in cultured organotypic skin grafts Plast. Reconstr. Surg. 96 166–176 Occurrence Handle1:STN:280:ByqA38jnsFY%3D Occurrence Handle7604097
G. Pellegrini R. Ranno G. Stracuzzi S. Bondanza L. Guerra G. Zambruno G. Micali M. Luca ParticleDe (1999) ArticleTitleThe control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin Transplantation 68 868–879 Occurrence Handle1:STN:280:DyaK1MvktlCisA%3D%3D Occurrence Handle10515389
H.O. Rennekampff J.F. Hansbrough V. Woods V. Kiessing (1996) ArticleTitleIntegrin and matrix molecule expression in cultured skin replacements J. Burn. Care Rehabil. 17 213–221 Occurrence Handle1:STN:280:BymA3sjlsFE%3D Occurrence Handle8736366
J.G. Rheinwald H. Green (1975) ArticleTitleSerial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells Cell 6 331–344 Occurrence Handle10.1016/0092-8674(75)90183-X Occurrence Handle1:STN:280:CSaD38zgvVc%3D Occurrence Handle1052771
R.L. Sheridan R.G. Tomkins (1995) ArticleTitleCultured autologous epithelium in patient with burns of ninety per cent or more of the body surface J. Trauma 38 48–50 Occurrence Handle1:STN:280:ByqB28vjvFM%3D Occurrence Handle7745657
T. Svensjo F. Yao B. Pomahac T. Winkler E. Eriksson (2002) ArticleTitleCultured autologous fibroblasts augment epidermal repair Transplantation 15 1033–1041
R.H. Walker (1990) Technical Manual EditionNumber10 American Association of Blood Banks Bethesda, MD
D. Wisser J. Steffes (2003) ArticleTitleSkin replacement with a collagen based dermal substituteautologous keratinocytes and fibroblasts in burn trauma Burns 29 375–380 Occurrence Handle1:STN:280:DC%2BD3s3ls1artQ%3D%3D Occurrence Handle12781618
D.T. Woodley H.D. Peterson S.R. Herzog (1988) ArticleTitleBurn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrils JAMA 259 2566–2571 Occurrence Handle10.1001/jama.259.17.2566 Occurrence Handle1:STN:280:BieC1cfivF0%3D Occurrence Handle3282083
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Llames, S., García, E., García, V. et al. Clinical Results of an Autologous Engineered Skin. Cell Tissue Banking 7, 47–53 (2006). https://doi.org/10.1007/s10561-004-7253-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10561-004-7253-4