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Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction

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Abstract

To test the feasibility and accuracy of myocardial contrast echocardiography (MCE) for predicting infarct size (IS) in a rat model of myocardial infarction (MI) and to compare a simplified single plane-based measurement of IS with the conventional three plane-based approach. Fifty male SD rats underwent left anterior descending artery ligation and were evaluated by MCE 8 h post MI. IS was calculated by the single and three plane-based approaches, compared to that determined by triphenyltetrazolium chloride (TTC) staining method. Simplified single plane-based MCE approach and TTC method showed similar IS values (38.48 ± 16.80% vs. 35.72 ± 15.33%, P > 0.05) and presented a favorable positive correlation (r = 0.851, P < 0.001). IS values derived from simplified single plane-based approach was also highly significantly correlated with that by the conventional MCE method (r = 0.973, P < 0.001). Bland–Altman plots also displayed satisfactory agreement between them. MCE was validated as a novel technique to quantify infarct area in rats with MI. A single measurement at the mid-papillary muscle level may become a simple, efficient and reliable approach for in vivo IS assessment.

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Authors and Affiliations

  1. Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China

    Xianghui Chen, Kai Cui, Jiancheng Xiu, Yi Lao, Daogang Zha, Jianping Bin & Yili Liu

  2. School of Pharmaceutical Science, Southern Medical University, Guangzhou, China

    Huanbing Lin

  3. Department of Pathophysiology, School of Basic Medicine, Southern Medical University, Guangzhou, China

    Biying Zhou

  4. School of Biomedical Engineering, Southern Medical University, Guangzhou, China

    Feixue Liang

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  1. Xianghui Chen
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  2. Kai Cui
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  3. Jiancheng Xiu
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  4. Huanbing Lin
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  5. Yi Lao
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  7. Feixue Liang
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  8. Daogang Zha
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  9. Jianping Bin
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  10. Yili Liu
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Correspondence to Yili Liu.

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Xianghui Chen and Kai Cui are co-first authors.

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Chen, X., Cui, K., Xiu, J. et al. Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction. Int J Cardiovasc Imaging 25, 713–716 (2009). https://doi.org/10.1007/s10554-009-9474-x

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  • DOI: https://doi.org/10.1007/s10554-009-9474-x

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