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Possibilities and limits of X-ray microtomography for in vivo and ex vivo detection of vascular calcifications

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Abstract

In the present paper, vascular calcifications due to chronic renal failure in rats are studied by X-ray microtomography (μ-CT). Although μ-CT is traditionally used as an imaging technique, a quantitative analysis of data obtained by in vivo and ex vivo μ-CT is described and discussed. By comparison with traditional destructive methods, such as histomorphometry and atomic absorption, the detection limits for calcium were determined in living rats and in extracted aortas. μ-CT proved to be an effective non-invasive imaging technique allowing non-destructive quantification of ectopic calcifications.

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References

  1. Davis GR, Wong FSL (1996) X-ray microtomography of bones and teeth. Physiol Meas 17:121–146. doi:10.1088/0967-3334/17/3/001

    Article  PubMed  CAS  Google Scholar 

  2. Paulus MJ, Gleason SS, Kennel SJ, Hunsicker PR, Johnson DK (2000) High resolution X-ray computed tomography : an emerging tool for small animal cancer research. Neoplasia 2:62–70. doi:10.1038/sj.neo.7900069

    Article  PubMed  CAS  Google Scholar 

  3. Ritman EL (2002) Molecular imaging in small animals: roles for μ-CT. J Cell Biochem 39(Supp):116–124. doi:10.1002/jcb.10415

    Article  Google Scholar 

  4. Namati E, Chon D, Thiesse J, Hoffman EA, de Ryk J, Ross A, McLennan G (2006) In vivo μ-CT lung imaging via a computer-controlled intermittent iso-pressure hold (IIBBH) technique. Phys Med Biol 51(23):6061–6075. doi:10.1088/0031-9155/51/23/008

    Article  PubMed  CAS  Google Scholar 

  5. Maréchal M, Luyten F, Nijs J, Postnov A, Schepers E, van Steenberghe D (2006) Histomorphometry and micro-computed tomography of bone augmentation under a titanium membrane. Clin Oral Implants Res 16(6):708–714. doi:10.1111/j.1600-0501.2005.01205.x

    Article  Google Scholar 

  6. De Clerck NM, Meurrens K, Weiler H, Van Dyck D, Vanhoutte G, Terpstra P, Postnov AA (2004) High resolution X-ray microtomography for the detection of lung tumors in living mice. Neoplasia 6:374–379. doi:10.1593/neo.03481

    Article  PubMed  Google Scholar 

  7. Postnov A, Meurrens K, Weiler H, Van Dyck D, Xu H, Terpstra P, De Clerck NM (2005) In vivo assessment of emphysema in mice by high resolution X-ray microtomography. J Microsc 220:70–75. doi:10.1111/j.1365-2818.2005.01510.x

    Article  PubMed  CAS  Google Scholar 

  8. Waarsing JH, Day JS, van der Linden JC, Ederveen AG, Spanjers C, De Clerck N, Sasov A, Weinans H (2004) Detecting and tracking local changes in the tibiae of individual rats : a novel method to analyse longitudinal in vivo μ-CT data. Bone 34(1):163–169. doi:10.1016/j.bone.2003.08.012

    Article  PubMed  CAS  Google Scholar 

  9. Postnov A, Zarowski A, De Clerck NM, Vanpoucke F, Offeciers W, Van Dyck D, Peeters S (2006) High Resolution M-CT scanning as innovatory tool for evaluation of the surgical positioning of cochlear implant electrodes. Acta Otolaryngol 126(5):467–474. doi:10.1080/00016480500437377

    Article  PubMed  CAS  Google Scholar 

  10. Nair MK, Nair UP (2007) Digital and advanced imaging in endodontics: a review. J Endod 33(1):1–6. doi:10.1016/j.joen.2006.08.013

    Article  PubMed  Google Scholar 

  11. Persy V, Postnov A, Neven E, Dams G, De Broe M, DHaese P, De Clerck NM (2006) Detection of vascular calcifications by high resolution X-ray microtomography in living rats with chronic renal failure. Arterioscler Thromb Vasc Biol 26:2110–2116

    Article  PubMed  CAS  Google Scholar 

  12. De Clerck N, Postnov A (2007) High resolution X-ray microtomography: applications in biomedical research. In: Tavitian B, Leroy-Willig A, Ntziachristos V (eds) International textbook of in vivo imaging in vertebrate. Wiley & Sons, London, pp 57–77

    Google Scholar 

  13. Katsumata K, Kusano K, Hirata M, Tsunemi K, Nagano N, Burke SK, Fukushima N (2003) Sevelamer hydrochloride prevents ectopic calcification and renal osteodystrophy in chronic renal failure rats. Kidney Int 64(2):441–450

    Article  PubMed  CAS  Google Scholar 

  14. Neven E, Dauwe S, De Broe ME, D’Haese PC, Persy V (2007) Endochondral bone formation is involved in media calcification in rats and in men. Kidney Int 72(5):574–581

    Article  PubMed  CAS  Google Scholar 

  15. Price PA, Roublick AM, Williamson MK (2006) Artery calcification in uremic rats is increased by a low protein diet and prevented by treatment with ibandronate. Kidney Int 70:1577–1583

    Article  PubMed  CAS  Google Scholar 

  16. Feldkamp LA, Davis LC, Kress JW (1984) Practical cone-beam algorithm. J Opt Soc Am A 1:612–619. doi:10.1364/JOSAA.1.000612

    Article  Google Scholar 

  17. Postnov A, Vinogradov A, Van Dyck D, Saveliev SV, De Clerck NM (2003) Quantitative analysis of bone mineral content by X-ray microtomography. Physiol Meas 24:165–178. doi:10.1088/0967-3334/24/1/312

    Article  PubMed  CAS  Google Scholar 

  18. Langheinrich A, Bohle R, Greschus S, Hackstein G, von Gerlach S, Rau W, Hoelschermann H (2004) Ahterosclerotic lesions at micro-CT: feasibility for analysis of coronary artery wall in autopsy specimens. Radiology 231:675–681. doi:10.1148/radiol.2313021718

    Article  PubMed  Google Scholar 

  19. Barreto M, Schoenhagen P, Nair A, Amatangelo S, Milite M, Obuchowski N, Lieber M, Halliburton S (2008) Potential of dual-energy computed tomography to characterize atherosclerotic plaque: ex vivo assessment of human coronary arteries in comparison to histology. J cardiovasc comput Tomogr 2:234–242

    Article  PubMed  Google Scholar 

  20. Schwarz F, Ruzzics B, Schoepf J, Bastarrika G, Chiarmida S, Abro J, Brothers Rm, Vogt S, Schmidt B, Costello P, Zwerner P (2008) Dual-energy CT of the heart—principles and protocols. Eur J Radiol 68:423–433. doi:10.1016/j.ejrad.2008.09.010

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Microtomography, University of Antwerp, Universiteitsplein 1, Campus Drie Eiken, Gebouw N, lokaal 010, 2610, Antwerp, Belgium

    A. A. Postnov & N. M. De Clerck

  2. Laboratory of Pathophysiology, University of Antwerp, Antwerp, Belgium

    P. C. D’Haese, E. Neven & V. P. Persy

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  1. A. A. Postnov
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  2. P. C. D’Haese
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  3. E. Neven
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  4. N. M. De Clerck
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  5. V. P. Persy
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Correspondence to A. A. Postnov.

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Postnov, A.A., D’Haese, P.C., Neven, E. et al. Possibilities and limits of X-ray microtomography for in vivo and ex vivo detection of vascular calcifications. Int J Cardiovasc Imaging 25, 615–624 (2009). https://doi.org/10.1007/s10554-009-9459-9

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  • DOI: https://doi.org/10.1007/s10554-009-9459-9

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