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Non-invasive analysis of myoblast transplants in rodent cardiac muscle

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Abstract

Background: Magnetic resonance imaging (MRI) of magnetically labeled stem cells is a non-invasive approach that can provide images with high spatial resolution. We evaluated the ability of a commercially available, Food and Drug Administration (FDA) approved contrast agent to allow the monitoring of myoblast transplants in the rodent heart. Methods and Results: Primary rat myoblasts were efficiently labeled by incubation with ferumoxide–polycation complexes and labeled cells retained their normal capacity to generate mature myotubes. Intra-cellular iron-oxide accumulation resulted in MRI contrast changes, allowing for three-dimensional, non-invasive detection of labeled cells in the rodent myocardium. Histological analysis of hearts injected with labeled myoblasts or control, non-viable myoblasts revealed that areas of MRI contrast changes corresponded to iron contained within engrafted myotubes and scavenger cells up to two months post-injection. Conclusions: The high sensitivity of MR imaging will allow for non-invasive studies of cardiac stem cell migration and homing. Additional techniques are in development to non-invasively determine stem cell engraftment rates, viability and differentiation.

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Abbreviations

AK:

arginine kinase

C:

Celsius

DMEM:

Dulbecco’s modified eagle’s medium

FBS:

fetal bovine serum

FDA:

food and drug administration

MRI:

magnetic resonance imaging

PBS:

phosphate buffered saline

PLL:

poly-l-lysine

SPIO:

superparamagnetic iron oxide

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Correspondence to Glenn A. Walter.

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Cahill, K.S., Germain, S., Byrne, B.J. et al. Non-invasive analysis of myoblast transplants in rodent cardiac muscle. Int J Cardiovasc Imaging 20, 593–598 (2004). https://doi.org/10.1007/s10554-004-3902-8

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  • DOI: https://doi.org/10.1007/s10554-004-3902-8

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