Single and multiple high-risk and low-risk Human Papillomavirus association with cervical lesions of 11,224 women in Jakarta
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We sought to evaluate prevalence, age-adjusted distribution, and impact of single and multiple high- and low-risk human papillomavirus (HPV) subtypes and their associations with cervical lesions.
Data were extracted from 11,224 women who underwent routine screening of HPV genotyping and liquid-based cytology co-testing. Fifteen high-risk (HR) and six low-risk (LR) HPV types were genotyped.
Overall HPV prevalence was 10.7 %, and young women (under 21 years old) harbored highest HPV infection rate (40.38 %). The rate declined in old women 9.49 % (age 30–49) and 6.89 % (age 50 and above). Normal cytology had lowest HPV (5.66 %) compared to low-grade (60.49 %), high-grade (71.96 %) squamous intraepithelial lesions (LSIL and HSIL) and squamous cell carcinoma SCC (86.9 %). LR HPV subtypes were absent in SCC and were consistently lower than HR HPV in LSIL (6.74 vs. 33.54 %) and HSIL (2.12 vs. 51.32 %). Multiple HPV infection was more frequent in young women under 30 years old (10 %) than older women (2 %) and in LSIL (20.2 %), HSIL (18.5 %) than SCC (4.4 %). HR HPV 52, 16, 18, and 58 were the most frequent subtypes in normal, LSIL, and HSIL. Greater or equal proportion of HPV 16, 18, 45, and 52 was found in SCC compared to normal cytology (SCC/normal ratios 4.8, 1.2, 1.6, and 1.7). While important in LSIL and HSIL, HPV58 was not detected in SCC.
Taken together, identification of these HPV types, especially HPV 16, 18, 45, and 52, and their associated cervical lesions may improve cervical cancer preventive strategies in Indonesia.
KeywordsHPV LBC HSIL LSIL Cervical cancer Genotyping Indonesia High-risk HPV Low-risk HPV Screening
Funding was provided to Stem Cell and Cancer Institute by Kalbe Farma (Internal Research Grant). We thanked Sartika Sari, Gunawan, Apryanti Masniary, Lusiana Mutiara, Shirliey Foo, Audi Tri Harsono and Yudhi Nugraha S. Putra for their excellent technical assistance, also Dr. Yong Wee Wong (DNA Laboratories Sdn Bhd, Malaysia) for the technical collaborations.
- 6.Chagas BS, Comar M, Gurgel APAD et al (2015) Association study between cervical lesions and single or multiple vaccine-target and non-vaccine target human papillomavirus (HPV) types in women from northeastern Brazil. PLoS One 10:e0132570. doi: 10.1371/journal.pone.0132570 CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Hosaka M, Fujita H, Hanley SJ et al (2012) Incidence risk of cervical intraepithelial neoplasia 3 or more severe lesions is a function of human papillomavirus genotypes and severity of cytological and histological abnormalities in adult Japanese women. Int J Cancer 132:327–334. doi: 10.1002/ijc.27680 CrossRefPubMedGoogle Scholar
- 15.Bruni L, Barrionuevo-Rosas L, Albero G, Serrano B, Mena M, Gómez D, Muñoz J, Bosch FX, de Sanjosé S. ICO Information Centre on HPV and Cancer (HPV Information Centre). Human Papillomavirus and Related Diseases in Indonesia. Summary Report 7 October 2016. Accessed 12 Oct 2016Google Scholar
- 17.Panigoro R, Susanto H, Novel SS et al (2013) HPV genotyping linear assay test comparison in cervical cancer patients: implications for HPV prevalence and molecular epidemiology in a limited-resource area in Bandung, Indonesia. Asian Pac J Cancer Prev 14:5843–5847. doi: 10.7314/APJCP.2013.14.10.5843 CrossRefPubMedGoogle Scholar