Skip to main content

Advertisement

SpringerLink
Log in

Validation of a food frequency questionnaire measurement of dietary acrylamide intake using hemoglobin adducts of acrylamide and glycidamide

  • Original Paper
  • Published:
Cancer Causes & Control Aims and scope Submit manuscript

Abstract

Objective

Acrylamide, a probable human carcinogen, is formed during high-heat cooking of many common foods. The validity of food frequency questionnaire (FFQ) measures of acrylamide intake has not been established. We assessed the validity of acrylamide intake calculated from an FFQ using a biomarker of acrylamide exposure.

Methods

We calculated acrylamide intake from an FFQ in the Nurses’ Health Study II. We measured hemoglobin adducts of acrylamide and its metabolite, glycidamide, in a random sample of 342 women. Correlation and regression analyses were used to assess the relationship between acrylamide intakes and adducts.

Results

The correlation between acrylamide intake and the sum of acrylamide and glycidamide adducts was 0.31 (95% CI: 0.20–0.41), adjusted for laboratory batch, energy intake, and age. Further adjustment for BMI, alcohol intake, and correction for random within-person measurement error in adducts gave a correlation of 0.34 (CI: 0.23–0.45). The intraclass correlation coefficient for the sum of adducts was 0.77 in blood samples collected 1–3 years apart in a subset of 45 women. Intake of several foods significantly predicted adducts in multiple regression.

Conclusions

Acrylamide intake and hemoglobin adducts of acrylamide and glycidamide were moderately correlated. Within-person consistency in adducts was high over time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. IARC(1994) Monographs on the evaluation of carcinogenic risks to humans, Vol 60

  2. International Programme on Chemical Safety. Acrylamide (EHC 49, 1985). Available at: www.inchem.org

  3. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M (2002) Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 50(17):4998–5006. doi:10.1021/jf020302f

    Article  PubMed  CAS  Google Scholar 

  4. Mucci LA, Dickman PW, Steineck G, Adami HO, Augustsson K (2003) Dietary acrylamide and cancer of the large bowel, kidney, and bladder: absence of an association in a population-based study in Sweden. Br J Cancer 88(1):84–89. doi:10.1038/sj.bjc.6600726

    Article  PubMed  CAS  Google Scholar 

  5. Mucci LA, Lindblad P, Steineck G, Adami HO (2004) Dietary acrylamide and risk of renal cell cancer. Int J Cancer 109(5):774–776. doi:10.1002/ijc.20011

    Article  PubMed  CAS  Google Scholar 

  6. Mucci LA, Sandin S, Balter K, Adami HO, Magnusson C, Weiderpass E (2005) Acrylamide intake and breast cancer risk in Swedish women. JAMA 293(11):1326–1327. doi:10.1001/jama.293.11.1326

    Article  PubMed  CAS  Google Scholar 

  7. Mucci LA, Adami HO, Wolk A (2006) Prospective study of dietary acrylamide and risk of colorectal cancer among women. Int J Cancer 118(1):169–173. doi:10.1002/ijc.21309

    Article  PubMed  CAS  Google Scholar 

  8. Pelucchi C, Franceschi S, Levi F, Trichopoulos D, Bosetti C, Negri E et al (2003) Fried potatoes and human cancer. Int J Cancer 105(4):558–560. doi:10.1002/ijc.11118

    Article  PubMed  CAS  Google Scholar 

  9. Pelucchi C, Galeone C, Levi F, Negri E, Franceschi S, Talamini R et al (2006) Dietary acrylamide and human cancer. Int J Cancer 118(2):467–471. doi:10.1002/ijc.21336

    Article  PubMed  CAS  Google Scholar 

  10. Pelucchi C, Galeone C, Dal Maso L, Talamini R, Montella M, Ramazzotti V et al (2007) Dietary acrylamide and renal cell cancer. Int J Cancer 120(6):1376–1377. doi:10.1002/ijc.22457

    Article  PubMed  CAS  Google Scholar 

  11. Hogervorst JG, Schouten LJ, Konings EJ, Goldbohm RA, van den Brandt PA (2007) A prospective study of dietary acrylamide intake and the risk of endometrial, ovarian, and breast cancer. Cancer Epidemiol Biomarkers Prev 16(11):2304–2313. doi:10.1158/1055-9965.EPI-07-0581

    Article  PubMed  CAS  Google Scholar 

  12. Stadler RH, Scholz G (2004) Acrylamide: an update on current knowledge in analysis, levels in food, mechanisms of formation, and potential strategies of control. Nutr Rev 62(12):449–467. doi:10.1301/nr.2004.janr.449-467

    Article  PubMed  Google Scholar 

  13. Wirfält E, Paulsson B, Törnqvist M, Axmon A, Hagmar L (2008) Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmo Diet and Cancer cohort. Eur J Clin Nutr 62(3):314–323

    Article  PubMed  CAS  Google Scholar 

  14. Bjellaas T, Olesen PT, Frandsen H, Haugen M, Stolen LH, Paulsen JE et al (2007) Comparison of estimated dietary intake of acrylamide with hemoglobin adducts of acrylamide and glycidamide. Toxicol Sci 98(1):110–117. doi:10.1093/toxsci/kfm091

    Article  PubMed  CAS  Google Scholar 

  15. Kütting B, Uter W, Drexler H (2008) The association between self-reported acrylamide intake and hemoglobin adducts as biomarkers of exposure. Cancer Causes Control 19(3):273–281

    Article  PubMed  Google Scholar 

  16. Bergmark E, Calleman CJ, He F, Costa LG (1993) Determination of hemoglobin adducts in humans occupationally exposed to acrylamide. Toxicol Appl Pharmacol 120(1):45–54. doi:10.1006/taap.1993.1085

    Article  PubMed  CAS  Google Scholar 

  17. Törnqvist M, Fred C, Haglund J, Helleberg H, Paulsson B, Rydberg P (2002) Protein adducts: quantitative and qualitative aspects of their formation, analysis and applications. J Chromatogr B Analyt Technol Biomed Life Sci 778(1–2):279–308. doi:10.1016/S1570-0232(02)00172-1

    PubMed  Google Scholar 

  18. U.S. Food and Drug Administration. Survey Data on Acrylamide in Food: Individual Food Products, 2002–2004. 2006; Available at: http://www.cfsan.fda.gov/~dms/acrydata.html

  19. U.S. Food and Drug Administration (2006) Survey Data on Acrylamide in Food: Total Diet Study Results, 2003–2006

  20. U.S. Food and Drug Administration. The 2006 Exposure Assessment for Acrylamide. 2006; Available at: http://www.cfsan.fda.gov/~dms/acryexpo.html

  21. Rosén J, Nyman A, Hellenäs KE (2007) Retention studies of acrylamide for the design of a robust liquid chromatography-tandem mass spectrometry method for food analysis. J Chromatogr A 1172(1):19–24. doi:10.1016/j.chroma.2007.09.050

    Article  PubMed  CAS  Google Scholar 

  22. Wenzl T, Karasek L, Rosen J, Hellenaes KE, Crews C, Castle L et al (2006) Collaborative trial validation study of two methods, one based on high performance liquid chromatography-tandem mass spectrometry and on gas chromatography-mass spectrometry for the determination of acrylamide in bakery and potato products. J Chromatogr A 1132(1–2):211–218. doi:10.1016/j.chroma.2006.07.007

    Article  PubMed  CAS  Google Scholar 

  23. Vesper HW, Ospina M, Meyers T, Ingham L, Smith A, Gray JG et al (2006) Automated method for measuring globin adducts of acrylamide and glycidamide at optimized Edman reaction conditions. Rapid Commun Mass Spectrom 20(6):959–964. doi:10.1002/rcm.2396

    Article  PubMed  CAS  Google Scholar 

  24. Vesper HW, Bernert JT, Ospina M, Meyers T, Ingham L, Smith A et al (2007) Assessment of the relation between biomarkers for smoking and biomarkers for acrylamide exposure in humans. Cancer Epidemiol Biomarkers Prev 16(11):2471–2478. doi:10.1158/1055-9965.EPI-06-1058

    Article  PubMed  CAS  Google Scholar 

  25. Willett WC (1998) Nutritional epidemiology. Oxford University Press, New York

    Google Scholar 

  26. Rosner B, Willett WC (1988) Interval estimates for correlation coefficients corrected for within-person variation: implications for study design and hypothesis testing. Am J Epidemiol 127(2):377–386

    PubMed  CAS  Google Scholar 

  27. Bergmark E (1997) Hemoglobin adducts of acrylamide and acrylonitrile in laboratory workers, smokers and nonsmokers. Chem Res Toxicol 10(1):78–84. doi:10.1021/tx960113p

    Article  PubMed  CAS  Google Scholar 

  28. Hagmar L, Wirfalt E, Paulsson B, Törnqvist M (2005) Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender. Mutat Res 580(1–2):157–165

    PubMed  CAS  Google Scholar 

  29. Schettgen T, Rossbach B, Kutting B, Letzel S, Drexler H, Angerer J (2004) Determination of haemoglobin adducts of acrylamide and glycidamide in smoking and non-smoking persons of the general population. Int J Hyg Environ Health 207(6):531–539. doi:10.1078/1438-4639-00324

    Article  PubMed  CAS  Google Scholar 

  30. Schettgen T, Weiss T, Drexler H, Angerer J (2003) A first approach to estimate the internal exposure to acrylamide in smoking and non-smoking adults from Germany. Int J Hyg Environ Health 206(1):9–14. doi:10.1078/1438-4639-00195

    Article  PubMed  CAS  Google Scholar 

  31. Aureli F, Di Pasquale M, Lucchetti D, Aureli P, Coni E (2007) An absorption study of dietary administered acrylamide in swine. Food Chem Toxicol 45(7):1202–1209. doi:10.1016/j.fct.2006.12.029

    Article  PubMed  CAS  Google Scholar 

  32. Doerge DR, Young JF, McDaniel LP, Twaddle NC, Churchwell MI (2005) Toxicokinetics of acrylamide and glycidamide in Fischer 344 rats. Toxicol Appl Pharmacol 208(3):199–209. doi:10.1016/j.taap.2005.03.003

    Article  PubMed  CAS  Google Scholar 

  33. Doerge DR, Young JF, McDaniel LP, Twaddle NC, Churchwell MI (2005) Toxicokinetics of acrylamide and glycidamide in B6C3F1 mice. Toxicol Appl Pharmacol 202(3):258–267. doi:10.1016/j.taap.2004.07.001

    Article  PubMed  CAS  Google Scholar 

  34. Besaratinia A, Pfeifer GP (2004) Genotoxicity of acrylamide and glycidamide. J Natl Cancer Inst 96(13):1023–1029

    Article  PubMed  CAS  Google Scholar 

  35. Manjanatha MG, Aidoo A, Shelton SD, Bishop ME, McDaniel LP, Lyn-Cook LE et al (2006) Genotoxicity of acrylamide and its metabolite glycidamide administered in drinking water to male and female Big Blue mice. Environ Mol Mutagen 47(1):6–17. doi:10.1002/em.20157

    Article  PubMed  CAS  Google Scholar 

  36. Calleman CJ, Bergmark E, Stern LG, Costa LG (1993) A nonlinear dosimetric model for hemoglobin adduct formation by the neurotoxic agent acrylamide and its genotoxic metabolite glycidamide. Environ Health Perspect 99:221–223. doi:10.2307/3431486

    Article  PubMed  CAS  Google Scholar 

  37. Vesper HW, Licea-Perez H, Meyers T, Ospina M, Myers GL (2005) Pilot study on the impact of potato chips consumption on biomarkers of acrylamide exposure. Adv Exp Med Biol 561:89–96

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the National Cancer Institute, CA050385. KMW is partially supported by NCI/NIH Training Grant T32 CA09001.

Author information

Authors and Affiliations

  1. Department of Epidemiology, Harvard School of Public Health, 665 Huntington Ave, Boston, MA, 02115, USA

    Kathryn M. Wilson & Walter C. Willett

  2. Department of Nutrition, Harvard School of Public Health, 665 Huntington Ave, Boston, MA, 02115, USA

    Kathryn M. Wilson, Paula Tocco, Laura Sampson & Walter C. Willett

  3. Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Hubert W. Vesper

  4. Swedish National Food Administration, Uppsala, Sweden

    Johan Rosén & Karl-Erik Hellenäs

  5. Department Environmental Chemistry, Stockholm University, Stockholm, Sweden

    Margareta Törnqvist

  6. Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

    Walter C. Willett

Authors
  1. Kathryn M. Wilson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hubert W. Vesper
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paula Tocco
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Laura Sampson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Johan Rosén
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Karl-Erik Hellenäs
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Margareta Törnqvist
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Walter C. Willett
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kathryn M. Wilson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wilson, K.M., Vesper, H.W., Tocco, P. et al. Validation of a food frequency questionnaire measurement of dietary acrylamide intake using hemoglobin adducts of acrylamide and glycidamide. Cancer Causes Control 20, 269–278 (2009). https://doi.org/10.1007/s10552-008-9241-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10552-008-9241-7

Keywords

Search

Navigation