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The importance of SOCS1 − 1478 CA/del polymorphism and expression in breast cancer: a case–control study in the north of Iran

  • Original Laboratory Investigation
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Breast Cancer Research and Treatment Aims and scope Submit manuscript

Abstract

Purpose

This project aimed to evaluate the relationship between the suppressor of cytokine signaling-1 (SOCS1) − 1478 CA > del genetic variation and breast cancer susceptibility. Moreover, we investigated the SOCS1 mRNA expression level in cancerous tissues.

Methods

A total of 100 patients with breast cancer and 120 healthy individuals were selected. Genomic DNA was extracted from blood. SOCS1 genotyping and relative gene expression were performed using ARMS-PCR (Amplification-Refractory Mutation System-Polymerase Chain Reaction) and real-time PCR, respectively.

Results

In breast cancer patients, the prevalence of genotype frequencies of SOCS1 (− 1478 CA > del) CA/CA, CA/del, and del/del was 52, 31, and 17%, respectively. Among controls, the distribution of CA/CA, CA/del, and del/del was 63, 15, and 22%, respectively. The chi-square test reported that a significant difference was observed in the genotypic distribution of SOCS1 (− 1478 CA > del) polymorphism between cases and controls (χ2 = 8.08, P = 0.01). In addition, the presence of the CA/del genotype was associated with an elevated risk of breast cancer (in the codominant model: OR 2.51; 95% CI 1.27–4.96, P = 0.007 and in the over dominant model: OR 2.54; 95% CI 1.32–4.90, P = 0.005). However, there was no significant difference in allelic distributions between the groups (P > 0.05). There was no significant difference in the breast cancer risk associated with the dominant and recessive genetic models when the reference was CA/CA and CA/CA + CA/del genotype, respectively (P = 0.09 and P = 0.38). Moreover, the expression of SOCS1 decreased in cancerous tissues as compared to the adjacent non-cancerous tissues (P < 0.0001).

Conclusion

In conclusion, a functional SOCS1 promoter polymorphism (− 1478 CA > del) may affect breast cancer susceptibility.

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Data availability

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

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Acknowledgements

This work was partially supported by the University of Guilan. The authors would like to thank everyone who participated in the genetic lab at the Faculty of Sciences in Rasht, Iran.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

    Habibbullah Paeiz, Zivar Salehi, Farhad Mashayekhi & Laleh Mirzanejad

  2. Department of Radiation Oncology, Cancer Research Center, Guilan University of Medical Sciences (GUMS), Rasht, Iran

    Hamid Saeidi Saedi

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [ZS, FM, HSS, HP]. The first draft of the manuscript was written by [ZS] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.”

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Correspondence to Zivar Salehi.

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This study was done in agreement with the Helsinki Declaration.

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Paeiz, H., Salehi, Z., Mashayekhi, F. et al. The importance of SOCS1 − 1478 CA/del polymorphism and expression in breast cancer: a case–control study in the north of Iran. Breast Cancer Res Treat 202, 389–395 (2023). https://doi.org/10.1007/s10549-023-07070-3

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