This study aimed to develop an ancestry-specific polygenic risk scores (PRSs) for the prediction of breast cancer events in Japanese females and validate it in a longitudinal cohort study.
Using publicly available summary statistics of female breast cancer genome-wide association study (GWAS) of Japanese and European ancestries, we, respectively, developed 31 candidate genome-wide PRSs using pruning and thresholding (P + T) and LDpred methods with varying parameters. Among the candidate PRS models, the best model was selected using a case-cohort dataset (63 breast cancer cases and 2213 sub-cohorts of Japanese females during a median follow-up of 11.9 years) according to the maximal predictive ability by Harrell’s C-statistics. The best-performing PRS for each derivation GWAS was evaluated in another independent case-cohort dataset (260 breast cancer cases and 7845 sub-cohorts of Japanese females during a median follow-up of 16.9 years).
For the best PRS model involving 46,861 single nucleotide polymorphisms (SNPs; P + T method with PT = 0.05 and R2 = 0.2) derived from Japanese-ancestry GWAS, the Harrell’s C-statistic was 0.598 ± 0.018 in the evaluation dataset. The age-adjusted hazard ratio for breast cancer in females with the highest PRS quintile compared with those in the lowest PRS quintile was 2.47 (95% confidence intervals, 1.64–3.70). The PRS constructed using Japanese-ancestry GWAS demonstrated better predictive performance for breast cancer in Japanese females than that using European-ancestry GWAS (Harrell’s C-statistics 0.598 versus 0.586).
This study developed a breast cancer PRS for Japanese females and demonstrated the usefulness of the PRS for breast cancer risk stratification.
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GWAS summary statistics of Japanese and European ancestry are available at http://jenger.riken.jp/en/result and https://bcac.ccge.medschl.cam.ac.uk/bcacdata/oncoarray/oncoarray-and-combined-summary-result/gwas-summary-results-breast-cancer-risk-2017/. Ethical guidelines issued by the Ministry of Health, Labor, and Welfare of Japan and the Ministry of Education, Culture, Sports, Science, and Technology of Japan ensure that the privacy of study participants prevents the public provision of individual data. Additionally, the informed consent we obtained did not include a provision for public data sharing. Researchers interested in verifying or replicating the results of our study can submit a research proposal to the Administration Office of the Japan Public Health Center-based prospective study at firstname.lastname@example.org. The study group board will follow a prescribed process to evaluate the importance of the research proposal in consideration of the study participants’ privacy and may provide special permission to access a minimal dataset.
Polygenic risk score
Genome-wide association studies
Single nucleotide polymorphism
Japan Public Health Center‐based
Public health center
Area under the receiver operating characteristic
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We thank the participants and staff involved in this study. The JPHC study members are listed on the following website: https://epi.ncc.go.jp/en/jphc/781/8896.html. We are indebted to the Aomori, Iwate, Akita, Ibaraki, Niigata, Osaka, Kochi, Nagasaki, and Okinawa Cancer Registries for providing the incidence data. We also thank Drs. Hiromi Sakamoto and Teruhiko Yoshida, staff at the Genetics Division, National Cancer Center Research Institute, and the Department of Clinical Genomics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute, and Drs. Yukihide Momozawa and Michiaki Kubo, and staff at the RIKEN Center for Integrative Medical Sciences.
This study was supported by the National Cancer Center Research and Development Fund (Grant Nos. 23-A-31 [toku], 26-A-2, 29-A-4, and 2020-J-4), Grant-in-Aid for Cancer Research from the Ministry of Health, Labour, and Welfare of Japan (Grant No. 19shi-2), and Practical Research for Innovative Cancer Control (Grant Nos. JP16ck0106095, JP19ck0106266, and JP22ck0106551) from the Japan Agency for Medical Research and Development. The funders had no role in the study design, data collection and analysis, decision to publish, or the preparation of the study.
Conflict of interest
TH is a board member of Genome Analytics Japan Inc.
The genetic research in the JPHC study, including this study, was approved by the Institutional Review Board of the National Cancer Center, Tokyo, Japan (Approval No.2011–044).
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Before the initiation of genetic research in the JPHC study, we announced its execution via our website and contacted all living blood donors by mail to provide them with the opportunity to opt out of participation in the research.
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Ohbe, H., Hachiya, T., Yamaji, T. et al. Development and validation of genome-wide polygenic risk scores for predicting breast cancer incidence in Japanese females: a population-based case-cohort study. Breast Cancer Res Treat 197, 661–671 (2023). https://doi.org/10.1007/s10549-022-06843-6
- Breast cancer
- Polygenic risk score
- Genome-wide association study