Breast Cancer Research and Treatment

, Volume 134, Issue 1, pp 353–362 | Cite as

Prevalence of BRCA1 mutations among 403 women with triple-negative breast cancer: implications for genetic screening selection criteria: a Hellenic Cooperative Oncology Group Study

  • Florentia Fostira
  • Marianthi Tsitlaidou
  • Christos Papadimitriou
  • Maroulio Pertesi
  • Eleni Timotheadou
  • Alexandra V. Stavropoulou
  • Stavros Glentis
  • Evangelos Bournakis
  • Mattheos Bobos
  • Dimitrios Pectasides
  • Pavlos Papakostas
  • George Pentheroudakis
  • Helen Gogas
  • Pantelis Skarlos
  • Epaminontas Samantas
  • Dimitrios Bafaloukos
  • Paris A. Kosmidis
  • Angelos Koutras
  • Drakoulis Yannoukakos
  • Irene Konstantopoulou
  • George Fountzilas
Epidemiology

Abstract

In spite the close association of the triple-negative breast cancer immunophenotype with hereditary breast cancers and the BRCA1 pathway, there is a lack of population studies that determine the frequency of BRCA1 mutations among triple-negative breast cancer patients. To address this, we have screened a large sample of 403 women diagnosed with triple-negative invasive breast cancer, independently of their age or family history, for germline BRCA1 mutations. Median age at diagnosis was 50 years (range 20–83). The overall prevalence of triple-negative cases among the initial patient group with invasive breast cancer was 8 %. BRCA1 was screened by direct DNA sequencing in all patients, including all exons where a mutation was previously found in the Greek population (exons 5, 11, 12, 16, 20, 21, 22, 23, 24–77 % of the BRCA1 coding region), including diagnostic PCRs to detect the three Greek founder large genomic rearrangements. Sixty-five deleterious BRCA1 mutations were identified among the 403 triple-negative breast cancer patients (16 %). Median age of onset for mutation carriers was 39 years. Among a total of 106 women with early-onset triple-negative breast cancer (<40 years), 38 (36 %) had a BRCA1 mutation, while 27 % of women with triple-negative breast cancer diagnosed before 50 years (56/208) had a BRCA1 mutation. A mutation was found in 48 % (50/105) of the triple-negative breast cancer patients with family history of breast or ovarian cancer. It is noteworthy, however, that of the 65 carriers, 15 (23 %) had no reported family history of related cancers. All but one of the carriers had grade III tumors (98 %). These results indicate that women with early-onset triple-negative breast cancer, and ideally all triple-negative breast cancer patients, are candidates for BRCA1 genetic testing even in the absence of a family history of breast or ovarian cancer.

Keywords

Triple-negative breast cancer BRCA1 Hereditary breast–ovarian cancer Genetic testing 

Abbreviations

ER

Estrogen receptor

PR

Progesterone receptor

HER2

Human epidermal growth factor receptor 2

PCR

Polymerase chain reaction

CI

Confidence interval

IHC

Immunohistochemistry

FISH

Fluorescence in situ hybridization

CISH

Chromogenic in situ hybridization

UV

Ultraviolet

PARP

Poly(ADP-ribose) polymerase

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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Florentia Fostira
    • 1
  • Marianthi Tsitlaidou
    • 1
  • Christos Papadimitriou
    • 2
  • Maroulio Pertesi
    • 1
  • Eleni Timotheadou
    • 3
  • Alexandra V. Stavropoulou
    • 1
  • Stavros Glentis
    • 1
  • Evangelos Bournakis
    • 2
  • Mattheos Bobos
    • 4
  • Dimitrios Pectasides
    • 5
  • Pavlos Papakostas
    • 6
  • George Pentheroudakis
    • 7
  • Helen Gogas
    • 8
  • Pantelis Skarlos
    • 9
  • Epaminontas Samantas
    • 10
  • Dimitrios Bafaloukos
    • 11
  • Paris A. Kosmidis
    • 12
  • Angelos Koutras
    • 13
  • Drakoulis Yannoukakos
    • 1
  • Irene Konstantopoulou
    • 1
  • George Fountzilas
    • 3
  1. 1.Molecular Diagnostics Laboratory, I/R-RPNational Center for Scientific Research “Demokritos”AthensGreece
  2. 2.Department of Clinical Therapeutics“Alexandra” Hospital, University of Athens School of MedicineAthensGreece
  3. 3.Department of Medical Oncology“Papageorgiou” Hospital, Aristotle University of Thessaloniki School of MedicineThessaloníki, MacedoniaGreece
  4. 4.Laboratory of Molecular Oncology, Hellenic Foundation for Cancer ResearchAristotle University of Thessaloniki School of MedicineThessaloníkiGreece
  5. 5.Oncology Section, Second Department of Internal Medicine“Hippokration” HospitalAthensGreece
  6. 6.Department of Medical Oncology“Hippokration” HospitalAthensGreece
  7. 7.Department of Medical OncologyIoannina University HospitalIoanninaGreece
  8. 8.First Department of Medicine“Laiko” General Hospital, University of Athens, Medical SchoolAthensGreece
  9. 9.Department of Radiotherapy“Agios Savvas” Cancer HospitalAthensGreece
  10. 10.Third Department of Medical Oncology“Agii Anargiri” Cancer HospitalAthensGreece
  11. 11.First Department of Medical Oncology“Metropolitan” HospitalAthensGreece
  12. 12.Second Department of Medical OncologyHygeia HospitalAthensGreece
  13. 13.Department of Medicine, Division of OncologyUniversity Hospital, University of Patras Medical SchoolPatrasGreece

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