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Association of CD27 and CD70 gene polymorphisms with risk of sporadic breast cancer in Chinese women in Heilongjiang Province

  • Epidemiology
  • Published:
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Abstract

CD27 and its ligand, CD70, are major costimulatory molecules whose interaction can regulate the expansion and differentiation of effector and memory T-cell populations. Their abnormal expression can disturb the immune response and lead to an increased risk of cancer. This study aims to evaluate the associations between single nucleotide polymorphisms (SNPs) in CD27/CD70 gene and breast cancer susceptibility. Five tagSNPs and one coding polymorphism in CD27, as well as three tagSNPs in CD70, were genotyped in a case–control study of 610 breast cancer patients and 617 healthy controls. In CD27, rs3136550 CT and rs2267966 AT genotypes were associated with a decreased risk of breast cancer (P = 0.03, OR = 0.76; P = 0.02, OR = 0.75, respectively). In CD70, AG and GG genotypes in rs1862511 and CC genotype in rs2059154 also showed significant associations with a decreased risk of breast cancer (P = 2.00 × 10−3, OR = 0.69; P = 0.03, OR = 0.62; P = 2.00 × 10−3, OR = 0.53; respectively). Significant associations were also found in the dominant and recessive models for rs2059154 and dominant model for rs1862511. In haplotype analysis, CCGAG haplotype in CD27 and TAA haplotype in CD70 conferred an increased risk of breast cancer (P = 5.60 × 10−3; P = 7.75 × 10−5, respectively), but TGC, TAC and TGA haplotypes in CD70 were associated with a decreased risk of breast cancer (P = 0.01; P = 5.2 × 10−3; P = 2.00 × 10−3, respectively). The associations of CCGAG, TAA, TAC and TGA haplotypes remained significant after correcting P value for multiple testing. Significant associations were shown between the SNPs of CD27 and lymph node metastasis, and ER and PR statuses. These results indicate that CD27 and CD70 gene polymorphisms may affect the risk of breast cancer and show that some SNPs are associated with breast cancer characteristics in a northern Chinese population.

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Abbreviations

CI:

Confidence interval

ER:

Estrogen receptor

Her-2:

Human epidermal growth factor receptor 2

HWE:

Hardy–Weinberg equilibrium

IDC:

Infiltrating duct carcinoma

LD:

Linkage disequilibrium

LN:

Lymph node

OR:

Odds ratio

PCR-RFLP:

Polymerase chain reaction-restriction fragment length polymorphism

PR:

Progesterone receptor

SNPs:

Single nucleotide polymorphisms

TZ:

Tumor size

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Acknowledgments

Fengyan Xu and Dalin Li designed the primers and wrote the drafts. Dalin Li collected the patient and control blood samples. Fengyan Xu, Qiujin Zhang and Zhenkun Fu performed the PCR-RFLP experiments. Fengyan Xu and Weiguang Yuan contributed to the statistical analysis. Da Pang and Dianjun Li conceived the study and participated in its design and coordination and helped draft the manuscript. All authors read and approved the final manuscript. This study was supported by a grant from the National Natural Science Foundation of China (31070780) and the Major Project of Technology Department, Heilongjiang Province (GB05C402). We thank all patients and healthy volunteers for providing blood samples. We are grateful for the collaboration received from the participating hospitals and their staff.

Conflicts of interests

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Cell Biology, Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, 150081, China

    Fengyan Xu, Qiujin Zhang, Zhenkun Fu, Weiguang Yuan, Da Pang & Dianjun Li

  2. Department of Immunology, Harbin Medical University, Harbin, 150081, China

    Fengyan Xu, Weiguang Yuan & Dianjun Li

  3. Department of Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150081, China

    Dalin Li & Da Pang

Authors
  1. Fengyan Xu
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  2. Dalin Li
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Correspondence to Da Pang or Dianjun Li.

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F. Xu and D. Li authors have contributed equally to this work.

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Xu, F., Li, D., Zhang, Q. et al. Association of CD27 and CD70 gene polymorphisms with risk of sporadic breast cancer in Chinese women in Heilongjiang Province. Breast Cancer Res Treat 133, 1105–1113 (2012). https://doi.org/10.1007/s10549-012-1987-7

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  • DOI: https://doi.org/10.1007/s10549-012-1987-7

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