Breast Cancer Research and Treatment

, Volume 110, Issue 1, pp 169–176 | Cite as

ERBB2 genetic polymorphism and breast cancer risk in Chinese women: a population-based case-control study

  • Shimian Qu
  • Qiuyin Cai
  • Yu-Tang Gao
  • Wei Lu
  • Hui Cai
  • Yinghao Su
  • Shizhen Emily Wang
  • Xiao-Ou Shu
  • Wei Zheng
Epidemiology

Abstract

A polymorphism at codon 655 (ATC/isoleucine to GTC/valine [Ile655Val], rs1801200) in the transmembrane domain-coding region of human ERBB2 gene has been previously evaluated for its association with breast cancer risk with mixed results. We evaluated this polymorphism in association with breast cancer in a group of women who participated in a large-scale, population-based, case-control study of breast cancer in Shanghai, China, followed by an in vitro analysis of the function of this polymorphism. Genomic DNA from 3,012 patients with breast cancer and 3,004 healthy controls was examined for the Ile655Val polymorphism using a TaqMan genotyping method. Adjusted odds ratios (OR) were derived from multiple logistic regression. In vitro analyses were carried out to examine whether the Ile655Val polymorphism affect ERBB2 expression and the activity of its downstream targets. Approximately 2% of study subjects carry the Val/Val genotype. Compared with women with the Ile/Ile (76%) genotype, women who had the Ile/Val (22%) or Val/Val genotype did not have an elevated risk of breast cancer. Stratified analyses by age and menopausal status revealed no apparent association with this polymorphism in any subgroups of women. In a serious of biochemical analyses, we found that the Ile655Val substitution did not alter ErbB2 and its downstream signaling molecule activity. These study results suggest that Ile655Val polymorphism of the ERBB2 gene do not alter its activity and may not be associated with increased breast cancer risk among Chinese women.

Keywords

ERBB2 Genetic polymorphism Breast cancer risk 

Abbreviations

CI

Confidence interval

OR

Odds ratios

BMI

Body mass index

EGFR

Epidermal growth factor receptor

ER

Estrogen receptors

MAPK

Mitogen-activated protein kinase

PI3K

Phosphatidylinositol-3 kinase

PR

Progesterone receptors

SBCS

Shanghai Breast Cancer Study

TNM

Tumor-node-metastasis

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Shimian Qu
    • 1
  • Qiuyin Cai
    • 1
  • Yu-Tang Gao
    • 2
  • Wei Lu
    • 3
  • Hui Cai
    • 1
  • Yinghao Su
    • 1
  • Shizhen Emily Wang
    • 4
  • Xiao-Ou Shu
    • 1
  • Wei Zheng
    • 1
  1. 1.Department of Medicine, Vanderbilt Epidemiology Center, Institute of Medicine and Public HealthVanderbilt University School of MedicineNashvilleUSA
  2. 2.Department of EpidemiologyShanghai Cancer InstituteShanghaiChina
  3. 3.Shanghai Center for Disease Control and PreventionShanghaiChina
  4. 4.Department of Cancer BiologyVanderbilt University School of MedicineNashvilleUSA

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