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Prevalence and prognostic and predictive relevance of PRAME in breast cancer

  • Epidemiology
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Abstract

Preferentially expressed antigen of melanoma (PRAME) has been described as a potential candidate for immunotherapeutic targeting. However, the prognostic and predictive relevance of PRAME in breast cancer has never been investigated. PRAME gene expression was evaluated in 103 breast tumour biopsies, using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Normal breast tissue was also analysed for comparative purposes. All qRT-PCRs were performed in triplicate. Kaplan–Meier survival curves, Chi-squared and Cox Regression analyses were used to identify associations between PRAME expression and patients’ clinicopathological and survival data. PRAME mRNA was detected in ∼53% of tumour specimens and 37% of normal breast specimens. Kaplan–Meier analysis showed expression of PRAME to correlate significantly with unfavourable disease outcome for patients, in terms of both their disease-free survival (p = 0.0004) and overall survival (OS) (p = 0.0052) times from diagnosis. Multivariate analysis indicated PRAME expression to be an independent prognostic factor for shortened disease-free survival (p = 0.026) and OS (p = 0.02). Furthermore, for patients who received adjuvant chemotherapy, significantly (p = 0.0291) shorter relapse-free survival was achieved for those whose tumour expressed PRAME, compared to those that did not express this transcript. Our results suggest that PRAME mRNA expression may be a useful prognostic and predictive marker for breast cancer.

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Acknowledgements

This work was supported by funding from Ireland’s Higher Educational Authority Programme for Research in Third Level Institutions (PRTLI) Cycle 3, Dublin City University’s Albert College Fellowship, Dublin City University’s Research Fellowship and the Health Research Board.

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Authors and Affiliations

  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, 9, Ireland

    Padraig Doolan, Martin Clynes, Jai Prakash Mehta, John Crown & Lorraine O’Driscoll

  2. St. Vincent’s University Hospital, Dublin, 4, Ireland

    Susan Kennedy & John Crown

Authors
  1. Padraig Doolan
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  2. Martin Clynes
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  3. Susan Kennedy
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  4. Jai Prakash Mehta
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  5. John Crown
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  6. Lorraine O’Driscoll
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Corresponding author

Correspondence to Lorraine O’Driscoll.

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Padraig Doolan and Martin Clynes contributed equally to this work.

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Doolan, P., Clynes, M., Kennedy, S. et al. Prevalence and prognostic and predictive relevance of PRAME in breast cancer. Breast Cancer Res Treat 109, 359–365 (2008). https://doi.org/10.1007/s10549-007-9643-3

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  • DOI: https://doi.org/10.1007/s10549-007-9643-3

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