Skip to main content
SpringerLink
Log in

Flexible-fabricated sensor module with programmable magnetic actuators coupled to L-cysteine functionalized Ag@Fe3O4 complexes for Cu2+ detection in fish tissues

  • Research
  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

Heavy metal contamination for seafood, particularly fish, is arising great concerns, and consequentially it is necessary to develop a simple and direct detection method. In this work, Ag@Fe3O4 is successfully prepared by simple solvothermal method, and we present a flexible-fabricated sensor module with assembled programmable magnetic actuators. The resulting sensor integrates a three-electrode system with two programmable magnetic actuators at the bottom of the device, which regulates the amount of current by adjusting the brake to control the adsorption force and vibration. The L-Cysteine functionalized Ag@Fe3O4 is coated on the surface of the electrode, then the Cu2+ is dropped into the reaction tank. Its performance is studied by cyclic voltammetry and electrochemical impedance spectroscopy, and the key experimental conditions such as deposition potential, deposition time, and electrolyte pH are gradually optimized. Under optimal conditions, Cu2+ can be detected over a wide linear range (0.01 ~ 4 μM) and at a low LOD (0.34 nM). The results show that the proposed method has a good application prospect in the detection of Cu2+. This method is successfully applied to Cu2+ analysis in fish samples with an acceptable recovery of 93 ~ 102%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data Availability

All data will be made available on request.

References

  • M. Atapour, G. Amoabediny, M. Ahmadzadeh-Raji, Integrated optical and electrochemical detection of Cu 2+ ions in water using a sandwich amino acid–gold nanoparticle-based nano-biosensor consisting of a transparent-conductive platform. RSC Adv. 9(16), 8882–8893 (2019)

    Article  Google Scholar 

  • H.D. Betts, O.M. Linder-Patton, C.J. Sumby,A Stable Coordination Polymer Based on Rod-Like Silver(I) Nodes with Contiguous Ag-S Bonding. 25(19), 4548(2020)

  • Y. Bi, G. Lu, Facile synthesis of platinum nanofiber/nanotube junction structures at room temperature. Chem. Mater. 20(4), 1224–1226 (2008)

    Article  Google Scholar 

  • J. Chen, Y. Liu, G. Zhu, A. Yuan, Ag@Fe3O4 nanowire: fabrication, characterization and peroxidase-like activity. Cryst. Res. Technol. 49(5), 309–314 (2014)

    Article  Google Scholar 

  • Y.-m Cheng, H.-b Fa, W. Yin, C.-j Hou, D.-q Huo, F.-m Liu, Y. Zhang, C. Chen, A sensitive electrochemical sensor for lead based on gold nanoparticles/nitrogen-doped graphene composites functionalized with l-cysteine-modified electrode. J. Solid State Electrochem. 20(2), 327–335 (2016)

    Article  Google Scholar 

  • S.W. Cho, A.S. Rao, S. Bhunia, Y.J. Reo, S. Singha, K.H. Ahn, Ratiometric fluorescence detection of Cu(II) with a keto-dipicolylamine ligand: A mechanistic implication. Sens. Actuators B Chem. 279, 204–212 (2019)

    Article  Google Scholar 

  • R. Dallinger, F. Prosi, H. Segner, H. Back, Contaminated food and uptake of heavy metals by fish: a review and a proposal for further research. Oecologia 73(1), 91–98 (1987)

    Article  Google Scholar 

  • H. Ebrahimi-Najafabadi, A. Pasdaran, R. RezaeiBezenjani, E. Bozorgzadeh, Determination of toxic heavy metals in rice samples using ultrasound assisted emulsification microextraction combined with inductively coupled plasma optical emission spectroscopy. Food Chem. 289, 26–32 (2019)

    Article  Google Scholar 

  • S. Fahad, H. Yu, L. Wang, A. Zain ul, M. Haroon, R.S. Ullah, A. Nazir, K.-u-R. Naveed, T. Elshaarani, A. Khan, Recent progress in the synthesis of silver nanowires and their role as conducting materials. J. Mater. Sci. 54(2), 997–1035 (2019)

    Article  Google Scholar 

  • S.L.C. Ferreira, M.A. Bezerra, A.S. Santos, W.N.L. dos Santos, C.G. Novaes, O.M.C. de Oliveira, M.L. Oliveira, R.L. Garcia, Atomic absorption spectrometry – A multi element technique. TrAC, Trends Anal. Chem. 100, 1–6 (2018)

    Article  Google Scholar 

  • M. Freitas, M. Sá Couto, M.F. Barroso, C. Pereira, N. de-los-Santos-Álvarez, A.J. Miranda-Ordieres, M.J. Lobo-Castañón, C. Delerue-Matos, Highly monodisperse Fe3O4@Au superparamagnetic nanoparticles as reproducible platform for genosensing genetically modified organisms. ACS Sensors 1(8), 1044–1053 (2016)

    Article  Google Scholar 

  • X.-C. Fu, J. Wu, J. Li, C.-G. Xie, Y.-S. Liu, Y. Zhong, J.-H. Liu, Electrochemical determination of trace copper(II) with enhanced sensitivity and selectivity by gold nanoparticle/single-wall carbon nanotube hybrids containing three-dimensional l-cysteine molecular adapters. Sens. Actuators, B Chem. 182, 382–389 (2013)

    Article  Google Scholar 

  • Z. Ghasemi, A. Mohammadi, Sensitive and selective colorimetric detection of Cu (II) in water samples by thiazolylazopyrimidine-functionalized TiO2 nanoparticles. Spectrochim. Acta A Mol. Biomol. Spectrosc. 239, 118554 (2020)

    Article  Google Scholar 

  • W. Guo, A. Umar, M.A. Alsaiari, L. Wang, M. Pei, Ultrasensitive and selective label-free aptasensor for the detection of penicillin based on nanoporous PtTi/graphene oxide-Fe3O4/ MWCNT-Fe3O4 nanocomposite. Microchem. J. 158, 105270 (2020)

    Article  Google Scholar 

  • A.T. Idowu, O.O. Igiehon, S. Idowu, O.O. Olatunde, S. Benjakul, Bioactivity potentials and general applications of fish protein hydrolysates. Int. J. Pept. Res. Ther. 27(1), 109–118 (2021)

    Article  Google Scholar 

  • S.J. Iyengar, M. Joy, C.K. Ghosh, S. Dey, R.K. Kotnala, S. Ghosh, Magnetic, X-ray and Mössbauer studies on magnetite/maghemite core–shell nanostructures fabricated through an aqueous route. RSC Adv. 4(110), 64919–64929 (2014)

    Article  Google Scholar 

  • M. Javed, N. Usmani, An overview of the adverse effects of heavy metal contamination on fish health. Proc. Natl. Acad. Sci. India Sect. B: Biol. Sci. 89(2), 389–403 (2019)

    Article  Google Scholar 

  • P. Jothimuthu, R.A. Wilson, J. Herren, E.N. Haynes, W.R. Heineman, I. Papautsky, Lab-on-a-chip sensor for detection of highly electronegative heavy metals by anodic stripping voltammetry. Biomed. Microdevice 13(4), 695–703 (2011)

    Article  Google Scholar 

  • S. KhaliliTilami, S. Sampels, Nutritional value of fish: lipids, proteins, vitamins, and minerals. Rev. Fish. Sci. Aquac. 26(2), 243–253s (2018)

    Article  Google Scholar 

  • J.A. Kim, H.J. Lee, H.-J. Kang, T.H. Park, The targeting of endothelial progenitor cells to a specific location within a microfluidic channel using magnetic nanoparticles. Biomed. Microdevice 11(1), 287–296 (2009a)

    Article  Google Scholar 

  • M.H. Kim, H.H. Jang, S. Yi, S.-K. Chang, M.S. Han, Coumarin-derivative-based off–on catalytic chemodosimeter for Cu2+ ions. Chem. Commun. 32, 4838–4840 (2009b)

    Article  Google Scholar 

  • X. Li, M. Zhang, Y. Hu, J. Xu, D. Sun, T. Hu, Z. Ni, Developing a versatile electrochemical platform with optimized electrode configuration through screen-printing technology toward glucose detection. Biomed. Microdevices 22(4), 74 (2020)

    Article  Google Scholar 

  • S.K. Moccelini, S.C. Fernandes, I.C. Vieira, Bean sprout peroxidase biosensor based on l-cysteine self-assembled monolayer for the determination of dopamine. Sens. Actuators B Chem. 133(2), 364–369 (2008)

    Article  Google Scholar 

  • X. Qin, H. Wang, Z. Miao, X. Wang, Y. Fang, Q. Chen, X. Shao, Synthesis of silver nanowires and their applications in the electrochemical detection of halide. Talanta 84(3), 673–678 (2011)

    Article  Google Scholar 

  • T.A.P. Rocha-Santos, Sensors and biosensors based on magnetic nanoparticles. TrAC Trends Anal. Chem. 62, 28–36 (2014)

    Article  Google Scholar 

  • D. Rohilla, S. Chaudhary, N. Kaur, A. Shanavas, Dopamine functionalized CuO nanoparticles: A high valued “turn on” colorimetric biosensor for detecting cysteine in human serum and urine samples. Mater. Sci. Eng., C 110, 110724 (2020)

    Article  Google Scholar 

  • J. Sha, C. Tong, H. Zhang, L. Feng, B. Liu, C. Lü, CdTe QDs functionalized mesoporous silica nanoparticles loaded with conjugated polymers: A facile sensing platform for cupric (II) ion detection in water through FRET. Dyes Pigm. 113, 102–109 (2015)

    Article  Google Scholar 

  • M. Shahjahan, K. Taslima, M.S. Rahman, M. Al-Emran, S.I. Alam, C. Faggio, Effects of heavy metals on fish physiology – a review. Chemosphere 300, 134519 (2022)

    Article  Google Scholar 

  • S. Shaik, A. Saminathan, D. Sharma, J.A. Krishnaswamy, D.R. Mahapatra, Monitoring microbial growth on a microfluidic lab-on-chip with electrochemical impedance spectroscopic technique. Biomed Microdevices. 23(2), 26 (2021)

    Article  Google Scholar 

  • H. Shamkhalichenar, C.J. Bueche, J.-W. Choi,Printed Circuit Board (PCB) Technology for Electrochemical Sensors and Sensing Platforms. 10(11), 159 (2020)

  • J. Shang, M. Zhao, H. Qu, H. Li, R. Gao, S. Chen, New application of p-n junction in electrochemical detection: The detection of heavy metal ions. J. Electroanal. Chem. 855, 113624 (2019)

    Article  Google Scholar 

  • M.K.L. Silva, I. Cesarino, Electrochemical sensor based on Sb nanoparticles/reduced graphene oxide for heavy metal determination. Int. J. Environ. Anal. Chem. 102(13), 3109–3123 (2022)

    Article  Google Scholar 

  • L. Song, M.G. Vijver, W.J.G.M. Peijnenburg, T.S. Galloway, C.R. Tyler, A comparative analysis on the in vivo toxicity of copper nanoparticles in three species of freshwater fish. Chemosphere 139, 181–189 (2015)

    Article  Google Scholar 

  • X. Su, H. Wang, C. Wang, X. Zhou, X. Zou, W. Zhang, Programmable dual-electric-field immunosensor using MXene-Au-based competitive signal probe for natural parathion-methyl detection. Biosens. Bioelectron. 214, 114546 (2022)

    Article  Google Scholar 

  • L. Suo, X. Dong, X. Gao, J. Xu, Z. Huang, J. Ye, X. Lu, L. Zhao, Silica-coated magnetic graphene oxide nanocomposite based magnetic solid phase extraction of trace amounts of heavy metals in water samples prior to determination by inductively coupled plasma mass spectrometry. Microchem. J. 149, 104039 (2019)

    Article  Google Scholar 

  • L. Wang, H. Qiu, C. Liang, P. Song, Y. Han, Y. Han, J. Gu, J. Kong, D. Pan, Z. Guo, Electromagnetic interference shielding MWCNT-Fe3O4@Ag/epoxy nanocomposites with satisfactory thermal conductivity and high thermal stability. Carbon 141, 506–514 (2019)

    Article  Google Scholar 

  • J. Wang, Q. Shan, X. Liang, F. Guan, Z. Zhang, H. Huang, H. Fang, Levels and human health risk assessments of heavy metals in fish tissue obtained from the agricultural heritage rice-fish-farming system in China. J. Hazard. Mater. 386, 121627 (2020)

    Article  Google Scholar 

  • W. Yang, J. Justingooding, D. Brynn Hibbert, Characterisation of gold electrodes modified with self-assembled monolayers of l-cysteine for the adsorptive stripping analysis of copper. J. Electroanal. Chem. 516(1), 10–16 (2001)

    Article  Google Scholar 

  • J. Yu, X. Zhang, M. Zhao, Y. Ding, Z. Li, Y. Ma, H. Li, H. Cui, Fabrication of the Ni-based composite wires for electrochemical detection of copper(II) ions. Anal. Chim. Acta 1143, 45–52 (2021)

    Article  Google Scholar 

  • Y. Yuan, S. Li, Y. Xue, J. Liang, L. Cui, Q. Li, S. Zhou, Y. Huang, G. Li, Y. Zhao, A Fe3O4@Au-basedpseudo-homogeneous electrochemical immunosensor for AFP measurement using AFP antibody-GNPs-HRP as detection probe. Anal. Biochem. 534, 56–63 (2017)

    Article  Google Scholar 

  • L. Yuan, X. Tian, Y. Fan, Z. Sun, K. Zheng, X. Zou, W. Zhang,TPB-DMTP@S-CDs/MnO2 Fluorescence Composite on a Dual-Emission-Capture Sensor Module for Fingerprint Recognition of Organophosphorus Pesticides. Analytical Chemistry. 95(5), 2741-2749 (2023)

  • Y. Zhang, X. Geng, J. Ai, Q. Gao, H. Qi, C. Zhang, Signal amplification detection of DNA using a sensor fabricated by one-step covalent immobilization of amino-terminated probe DNA onto the polydopamine-modified screen-printed carbon electrode. Sens. Actuators B Chem. 221, 1535–1541 (2015)

    Article  Google Scholar 

  • X. Zhou, Z. Sun, X. Su, K. Zheng, X. Zou, W. Zhang, Ratiometric detection of Ochratoxin A using a regenerable COF-Au-MB-Apt signal probe on a thermal-regulated sensor module. Anal. Chem. 95(3), 1916–1923 (2023)

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by National Natural Science Foundation of China (32102080, 31801631, 31671844, 1601360061); Natural Science Foundation of Jiangsu Province (BK20160506, BK20180865); Post-graduate Research & Practice Innovation Program of Jiangsu Province (KYCX21_3395).

Author information

Authors and Affiliations

  1. Department of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Kuiguo Han, Wen Zhang, Xiaobo Zou, Jiyong Shi & Xiaoyu Su

  2. Department of Mechanical Engineering, Jiangsu University, Zhenjiang, 212013, China

    Bin Jiang & Yanqun Tong

Authors
  1. Kuiguo Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanqun Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaobo Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jiyong Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiaoyu Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Kuiguo Han: formal analysis and investigation.Bin Jiang: methodology and writing-original draft. Yanqun Tong: Methdology. Wen Zhang: writing-review&editing. Xiaobo Zou and Jiyong Shi: conceptualization and data curation project administration. Xiaoyu Su:supervision and review.

Corresponding author

Correspondence to Xiaobo Zou.

Ethics declarations

Competing interests

The authors declare no competing interests.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 339 kb)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Han, K., Jiang, B., Tong, Y. et al. Flexible-fabricated sensor module with programmable magnetic actuators coupled to L-cysteine functionalized Ag@Fe3O4 complexes for Cu2+ detection in fish tissues. Biomed Microdevices 25, 15 (2023). https://doi.org/10.1007/s10544-023-00654-2

Download citation

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10544-023-00654-2

Keywords

Search

Navigation