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Drug release evaluation of Paclitaxel/Poly-L-Lactic acid nanoparticles based on a microfluidic chip

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Abstract

Paclitaxel is a commonly used drug in the medical field because of its strong anticancer effect. However, it may produce relatively severe side effects (i.e., allergic reactions). A major characteristic of paclitaxel is low solubility in water. Special solvents are used for dissolving paclitaxel and preparing the paclitaxel drugs, while the solvents themselves will cause certain effects. Polyoxyethylene castor oil, for example, can cause severe allergic reactions in some people, and the clinical use is limited. In this study, we developed a new Paclitaxel/Poly-L-Lactic Acid (PLLA) nanoparticle drug, which is greatly soluble in water, and carried out in vitro drug sustained release research on it and the original paclitaxel drug. However, because the traditional polymer drug carrier usually uses dialysis bag and thermostatic oscillation system to measure the drug release degree in vitro, the results obtained are greatly different from the actual drug release results in human body. Therefore, this paper adopts the microfluidic chip we previously developed to mimic the human blood vessels microenvironment to study the sustained-release of Paclitaxel/PLLA nanoparticles to make the results closer to the release value in human body. The experimental results showed that compared with the original paclitaxel drug, Paclitaxel/PLLA nanoparticles have a long-sustained release time and a slow drug release, realizing the sustained low-dose release of paclitaxel, a cell cycle-specific anticancer drug, and provided certain reference significance and theoretical basis for the research and development of anticancer drugs.

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Acknowledgements

This work was financially supported by the International Science & Technology Cooperation Program of China (No.2015DFA30550), the Henan Provincial Natural Science Foundation (No.162300410245), the Major Science and Technology Special Project of Henan Province (No.171100210600), the Program of China Scholarship Council (No.201807045057) and the High Level Talent Internationalization Training Program of Henan Province (No.2019004).

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Authors and Affiliations

  1. School of Mechanics & Safety Engineering, Zhengzhou University, Zhengzhou, 450001, China

    Xiang Zhang, Guotao Guan, Zhenxing Wang, Zhenhao Yan, Shujie Yan, Lixia Wang & Qian Li

  2. Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA

    Xiang Zhang, Li Lv, Carolina Chávez-Madero & Mo Chen

  3. Department of Pharmacy, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China

    Li Lv

  4. Departamento de Ingeniería Mecatrónica Y Electrónica, Escuela de Ingeniería Y Ciencias, Tecnologico de Monterrey, Monterrey, 64849, NL, México

    Carolina Chávez-Madero

  5. Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China

    Mo Chen

  6. National Center for International Joint Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, 450001, China

    Xiang Zhang, Guotao Guan, Zhenxing Wang, Zhenhao Yan, Shujie Yan, Lixia Wang & Qian Li

  7. Key Laboratory for Micro Molding Technology of Henan Province, Zhengzhou University, Zhengzhou, 450001, China

    Xiang Zhang, Guotao Guan, Zhenxing Wang, Zhenhao Yan, Shujie Yan, Lixia Wang & Qian Li

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  1. Xiang Zhang
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Zhang, X., Guan, G., Wang, Z. et al. Drug release evaluation of Paclitaxel/Poly-L-Lactic acid nanoparticles based on a microfluidic chip. Biomed Microdevices 23, 57 (2021). https://doi.org/10.1007/s10544-021-00596-7

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