Self-assembled silver nanoparticle-DNA on a dielectrode microdevice for determination of gynecologic tumors

Abstract

Nanoscale materials have been employed in the past 2 decades in applications such as biosensing, therapeutics and medical diagnostics due to their beneficial optoelectronic properties. In recent years, silver nanoparticles (AgNPs) have gained attention due to their higher plasmon excitation efficiency than gold nanoparticles, as proved by sharper and stronger plasmon resonance peaks. The current work is focused on utilizing self-assembled DNA-AgNPs on microdevices for the detection of gynecological cancers. Human papilloma virus (HPV) mostly spreads through sexual transmittance and can cause various gynecological cancers, including cervical, ovarian and endometrial cancers. In particular, oncogene E7 from the HPV strain 16 (HPV-16 E7) is responsible for causing these cancers. In this research, the target sequence of HPV-16 E7 was detected by an AgNP-conjugated capture probe on a dielectrode sensor. The detection limit was in the range between 10 and 100 aM (by 3σ estimation). The sensitivity of the AgNP-conjugated probe was 10 aM and similar to the sensitivity of gold nanoparticle conjugation sensors, and the mismatched control DNA failed to detect the target, proving selective HPV detection. Morphological assessments on the AgNPs and the sensing surfaces by high-resolution microscopy revealed the surface arrangement. This sensing platform can be expanded to develop sensors for the detection various clinically relevant targets.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

References

  1. P. Anbu, S.C.B. Gopinath, H.S. Yun, C.-G. Lee, J. Mol. Struct. 1177, 302 (2018)

    Article  Google Scholar 

  2. N. Azizah, U. Hashim, S.C.B. Gopinath, S. Nadzirah, Microchim. Acta 183, 3119 (2016)

    Article  Google Scholar 

  3. K. Bankura, D. Maity, M.M.R. Mollick, D. Mondal, B. Bhowmick, I. Roy, T. Midya, J. Sarkar, D. Rana, K. Acharya, D. Chattopadhyay, Carbohydr. Polym. 107, 151 (2014)

    Article  Google Scholar 

  4. X. Bao, G. Huo, L. Li, X. Cao, Y. Liu, T. Lakshmipriya, Y. Chen, F. Hariri, S.C.B. Gopinath, J. Anal. Methods Chem. 2019, 5676159 (2019)

    Google Scholar 

  5. D.S. Campos-Ferreira, G.A. Nascimento, E.V.M. Souza, M.A. Souto-Maior, M.S. Arruda, D.M.L. Zanforlin, M.H.F. Ekert, D. Bruneska, J.L. Lima-Filho, Anal. Chim. Acta 804, 258 (2013)

    Article  Google Scholar 

  6. D.S. Campos-Ferreira, E.V.M. Souza, G.A. Nascimento, D.M.L. Zanforlin, M.S. Arruda, M.F.S. Beltrão, A.L. Melo, D. Bruneska, J.L. Lima-Filho, Arab. J. Chem. 9, 443 (2016)

    Article  Google Scholar 

  7. L. Civit, A. Fragoso, C.K. O’Sullivan, Biosens. Bioelectron. 26, 1684 (2010)

    Article  Google Scholar 

  8. G.S. Dorraj, M.J. Rassaee, A.M. Latifi, B. Pishgoo, M. Tavallaei, J. Biotechnol. 208, 80 (2015)

    Article  Google Scholar 

  9. M. Holzinger, A. Le Goff, S. Cosnier, 2, 1 (2014)

  10. K.J. Huang, Y.J. Liu, J.Z. Zhang, J.T. Cao, Y.M. Liu, Biosens. Bioelectron. 67, 184 (2015)

    Article  Google Scholar 

  11. S. Jampasa, W. Wonsawat, N. Rodthongkum, W. Siangproh, P. Yanatatsaneejit, T. Vilaivan, O. Chailapakul, Biosens. Bioelectron. 54, 428 (2014)

    Article  Google Scholar 

  12. N. Kasyanenko, M. Varshavskii, E. Ikonnikov, E. Tolstyko, R. Belykh, P. Sokolov, V. Bakulev, V. Rolich, K. Lopatko, J. Nanomater. (2016)

  13. T. Lakshmipriya, Y. Horiguchi, Y. Nagasaki, Analyst 139, 3977 (2014)

    Article  Google Scholar 

  14. D.D. Le, T.N.N. Nguyen, D.C.T. Doan, T.M.D. Dang, M.C. Dang, Adv. Nat. Sci. Nanosci. Nanotechnol. (2016)

  15. I. Letchumanan, M.K. Md Arshad, S.R. Balakrishnan, S.C.B. Gopinath, Biosens. Bioelectron. 130, 40 (2019)

    Article  Google Scholar 

  16. J. Lin, S.C.B. Gopinath, T. Lakshmipriya, Y. Chen, W.R. Yuan, M. Yang, Int. J. Biol. Macromol. 141, 564 (2019)

    Article  Google Scholar 

  17. Q. Lv, Y. Wang, C. Su, T. Lakshmipriya, S.C.B. Gopinath, K. Pandian, V. Perumal, Y. Liu, Int. J. Biol. Macromol. 134, 354 (2019)

    Article  Google Scholar 

  18. X. Mao, Y. Ma, A. Zhang, L. Zhang, L. Zeng, G. Liu, Anal. Chem. (2009)

  19. C.C. Ong, S.C.B. Gopinath, L.W.X. Rebecca, V. Perumal, T. Lakshmipriya, M.S.M. Saheed, Int. J. Biol. Macromol. 116, 765 (2018)

    Article  Google Scholar 

  20. P. Velusamy, C.-H. Su, G. Venkat Kumar, S. Adhikary, K. Pandian, S.C.B. Gopinath, Y. Chen, P. Anbu, PLoS One 11, e0157612 (2016)

    Article  Google Scholar 

  21. S. Wang, L. Li, H. Jin, T. Yang, W. Bao, S. Huang, J. Wang, Biosens. Bioelectron. 41, 205 (2013)

    Article  Google Scholar 

  22. H. Wang, T. Lakshmipriya, Y. Chen, S.C.B. Gopinath, Biomed. Res. Int. 2019, 1 (2019)

    Google Scholar 

  23. L. Yang, Y. Li, Biosens. Bioelectron. 20, 1407 (2005)

    Article  Google Scholar 

  24. N. Yang, L. WeiHong, L. Hao, Mater. Lett. 134, 67 (2014)

    Article  Google Scholar 

  25. S. Zheng, H. Zhang, T. Lakshmipriya, S.C.B. Gopinath, N. Yang, Biomed. Res. Int. 2019, 9726967 (2019)

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Xizhen Wang.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Li, Z., Gopinath, S.C.B., Lakshmipriya, T. et al. Self-assembled silver nanoparticle-DNA on a dielectrode microdevice for determination of gynecologic tumors. Biomed Microdevices 22, 67 (2020). https://doi.org/10.1007/s10544-020-00522-3

Download citation

Keywords

  • DNA sensor
  • Cervical cancer
  • Interdigitated electrode sensor
  • Human papilloma virus