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Self-assembled silver nanoparticle-DNA on a dielectrode microdevice for determination of gynecologic tumors

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Abstract

Nanoscale materials have been employed in the past 2 decades in applications such as biosensing, therapeutics and medical diagnostics due to their beneficial optoelectronic properties. In recent years, silver nanoparticles (AgNPs) have gained attention due to their higher plasmon excitation efficiency than gold nanoparticles, as proved by sharper and stronger plasmon resonance peaks. The current work is focused on utilizing self-assembled DNA-AgNPs on microdevices for the detection of gynecological cancers. Human papilloma virus (HPV) mostly spreads through sexual transmittance and can cause various gynecological cancers, including cervical, ovarian and endometrial cancers. In particular, oncogene E7 from the HPV strain 16 (HPV-16 E7) is responsible for causing these cancers. In this research, the target sequence of HPV-16 E7 was detected by an AgNP-conjugated capture probe on a dielectrode sensor. The detection limit was in the range between 10 and 100 aM (by 3σ estimation). The sensitivity of the AgNP-conjugated probe was 10 aM and similar to the sensitivity of gold nanoparticle conjugation sensors, and the mismatched control DNA failed to detect the target, proving selective HPV detection. Morphological assessments on the AgNPs and the sensing surfaces by high-resolution microscopy revealed the surface arrangement. This sensing platform can be expanded to develop sensors for the detection various clinically relevant targets.

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Authors and Affiliations

  1. Department of Gynecology, Hanzhong Central Hospital, Hanzhong City, 723000, Shaanxi Province, China

    Zhao Li

  2. Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath

  3. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    Subash C. B. Gopinath & Thangavel Lakshmipriya

  4. Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea

    Periasamy Anbu

  5. Mechanical Engineering Department, Universiti Teknologi PETRONAS, 32610, Seri Iskandar, Perak Darul Ridzuan, Malaysia

    Veeradasan Perumal

  6. Department of Laboratory, The Second People’s Hospital of Lianyungang, Lianyungang, 222000, Jiangsu, China

    Xizhen Wang

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  1. Zhao Li
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  2. Subash C. B. Gopinath
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  3. Thangavel Lakshmipriya
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  4. Periasamy Anbu
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  6. Xizhen Wang
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Correspondence to Xizhen Wang.

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Li, Z., Gopinath, S.C.B., Lakshmipriya, T. et al. Self-assembled silver nanoparticle-DNA on a dielectrode microdevice for determination of gynecologic tumors. Biomed Microdevices 22, 67 (2020). https://doi.org/10.1007/s10544-020-00522-3

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  • DOI: https://doi.org/10.1007/s10544-020-00522-3

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