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Monitoring drug pharmacokinetics and immunologic biomarkers in dermal interstitial fluid using a microneedle patch

Abstract

Minimally invasive point-of-care diagnostic devices are of great interest for rapid detection of biomarkers in diverse settings. Although blood is the most common source of biomarkers, interstitial fluid (ISF) is an alternate body fluid that does not clot or contain red blood cells that often complicate analysis. However, ISF is difficult to collect. In this study, we assessed the utility of a microneedle patch to sample microliter volumes of ISF in a simple and minimally invasive manner. We demonstrated the use of ISF collected in this way for therapeutic drug monitoring by showing similar vancomycin pharmacokinetic profiles in ISF and serum from rats. We also measured polio-specific neutralizing antibodies and anti-polio IgG in ISF similar to serum in rats immunized with polio vaccine. These studies demonstrate the potential utility of ISF collected by microneedle patch in therapeutic drug monitoring and immunodiagnostic applications.

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Acknowledgments

The authors would like to thank William Weldon of the CDC for conducting serum and ISF neutralizing antibody assays and Donna Bondy for administrative support. Mark Prausnitz is an inventor of patents that have been or may be licensed to companies developing microneedle-based products, is a paid advisor to companies developing microneedle-based products and is a founder/shareholder of companies developing microneedle-based products, including Micron Biomedical. These potential conflicts of interest have been disclosed and are being managed by Georgia Tech and/or Emory University.

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Correspondence to Mark R. Prausnitz.

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Kolluru, C., Williams, M., Yeh, J.S. et al. Monitoring drug pharmacokinetics and immunologic biomarkers in dermal interstitial fluid using a microneedle patch. Biomed Microdevices 21, 14 (2019). https://doi.org/10.1007/s10544-019-0363-3

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  • DOI: https://doi.org/10.1007/s10544-019-0363-3

Keywords

  • Interstitial fluid
  • Microneedle patch
  • Biomarker
  • Vancomycin
  • Polio specific antibodies
  • Therapeutic drug monitoring