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On chip quadruplex priming amplification for quantitative isothermal diagnostics

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Abstract

Nucleic acid testing is a common technique for medical diagnostics. For example, it is used to detect HIV treatment failure by monitoring viral load levels. Quadruplex Priming Amplification (QPA) is an isothermal nucleic acid amplification technique that requires little power and few chemical reagents per assay, all features that make QPA well suited for point-of-care (POC) diagnostics. The QPA assay can be further optimized by integrating it with microfluidic devices that can automate and combine multiple reaction steps and reduce the quantity and cost of reagents per test. In this study, a real-time, exponential QPA reaction is demonstrated for the first time in a microfluidic chip, where the reaction was not inhibited and supported performance levels comparable to a commercially-available, non-microfluidics setup.

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Acknowledgements

The authors gratefully acknowledge the financial support provided by the Ohio State University Public Health Preparedness for Infectious Diseases Program (OSU-PHPID).

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Authors and Affiliations

  1. Department of Physics, The Ohio State University, Columbus, OH, 43210, USA

    C. Pease & R. Sooryakumar

  2. IBET Inc., Columbus, OH, 43220, USA

    G. E. Plum

  3. Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA

    B. Kankia

  4. Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA

    J. J. Kwiek

Authors
  1. C. Pease
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  2. G. E. Plum
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  3. B. Kankia
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  4. J. J. Kwiek
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  5. R. Sooryakumar
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Correspondence to R. Sooryakumar.

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Pease, C., Plum, G.E., Kankia, B. et al. On chip quadruplex priming amplification for quantitative isothermal diagnostics. Biomed Microdevices 20, 56 (2018). https://doi.org/10.1007/s10544-018-0305-5

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  • DOI: https://doi.org/10.1007/s10544-018-0305-5

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