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A compact and integrated immunoassay with on-chip dispensing and magnetic particle handling

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Abstract

We present a compact diagnostic platform for a rapid and sensitive detection of plasma biomarkers. The platform consists of a disposable microfluidic polymer chip, a processing device including a lens-free and cost efficient sensor system and a setup for dispersion of magnetic particles. The biomarkers of interest are quantified by magnetic bead based immunoassays with chemiluminescent readout technology. With a novel system for dispersion and manipulation of the magnetic particles in combination with chemiluminescence detection, the sensitivity of the immunoassay is improved and enables a rapid assay in a microfluidic format. In the disposable chip, extra chambers for storage and dispensing of biomarker specific reagents are integrated, which reduce the need of external dosing devices and thereby the cost of the platform is decreased. Plasma biomarkers for monitoring of sepsis could be quantified at 10 pg/mL concentrations within a total time of 30 min by the present system. This contribution is a fundamental step towards the development of an automatic and compact Point-of-Care testing device for monitoring of patients at the intensive care unit.

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Acknowledgments

This research is supported by the FFG-Bridge Programme and is a part of the project “Entwicklung eines innovativen Diagnosesystems für das Bed-Side Immunomonitoring - BSI” (829651). The authors would like to acknowledge Peter Emminger at Biegler GmbH for his support during design and construction of the processing device and Mohammad Jafarmadar and Soheyl Bahrami for valuable discussions regarding development of the immunoassay and Point-of-Care diagnosis.

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Authors and Affiliations

  1. Molecular Diagnostics, Health and Environment Department, Austrian Institute of Technology, Muthgasse 11/2, Vienna, Austria

    Helene Zirath & Johannes R. Peham

  2. Biegler GmbH, Allhangstrasse 18a, 3001, Mauerbach, Austria

    Guntram Schnetz

  3. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, Vienna, Austria

    Helene Zirath, Albert Coll & Heinz Redl

  4. Inst. for Microsensors, −Actuators, & -Syst. (IMSAS/MCB), University of Bremen, Bremen, Germany

    Lukas Brandhoff & Michael J. Vellekoop

  5. Surgical Research Laboratories and Core Facility Flow Cytometry, Medical University of Vienna, Vienna, Austria

    Andreas Spittler

Authors
  1. Helene Zirath
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  2. Johannes R. Peham
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  3. Guntram Schnetz
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  4. Albert Coll
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  5. Lukas Brandhoff
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  6. Andreas Spittler
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  7. Michael J. Vellekoop
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  8. Heinz Redl
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Correspondence to Helene Zirath.

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Zirath, H., Peham, J.R., Schnetz, G. et al. A compact and integrated immunoassay with on-chip dispensing and magnetic particle handling. Biomed Microdevices 18, 16 (2016). https://doi.org/10.1007/s10544-016-0045-3

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  • DOI: https://doi.org/10.1007/s10544-016-0045-3

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