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Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells

Biomedical Microdevices volume 17, Article number: 116 (2015) Cite this article

Abstract

The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 – 30 °), RLs (5 – 500 μm), RWs (5 – 500 μm), and GWs (5 – 500 μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface.

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Acknowledgments

This study was funded by collaborative research funding from SEIKOH GIKEN Co., Ltd.

Author information

Affiliations

  1. Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Xiangkai Zhang, Tomoki Aoyama, Akira Ito, Junichi Tajino, Momoko Nagai, Rune Fujioka, Hirotaka Iijima, Shoki Yamaguchi & Hiroshi Kuroki

  2. Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan

    Takashi Yasuda, Makoto Oike & Norihiro Kakinuma

Authors
  1. Xiangkai Zhang
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  2. Tomoki Aoyama
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  3. Takashi Yasuda
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  4. Makoto Oike
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  5. Akira Ito
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  6. Junichi Tajino
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  7. Momoko Nagai
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  9. Hirotaka Iijima
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  10. Shoki Yamaguchi
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  11. Norihiro Kakinuma
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  12. Hiroshi Kuroki
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Corresponding author

Correspondence to Tomoki Aoyama.

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Conflict of interest

Takashi Yasuda, Makoto Oike, and Norihiro Kakinuma are employees of SEIKOH GIKEN Co., Ltd.

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Supplementary Fig. 1
figure 11

Cells on the non-microgroove. Magnification, 40×. Scale bar, 100 μm (GIF 2087 kb)

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Supplementary Fig. 2
figure 12

Immunohistochemical staining of cells, at 80 % confluence, cultured on the No. 2 of the RA pattern dish. (a) Magnification, 10×, Scale bar, 100 μm (b) Magnification, 60×, Scale bar, 100 μm (3) Magnification, 150×, Scale bar, 100 μm. White arrow indicates the change of F-actin in filopodia of cell. White arrowhead indicates the change of F-actin in the cell body. Ridge angle (RA) (GIF 98 kb)

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Zhang, X., Aoyama, T., Yasuda, T. et al. Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells. Biomed Microdevices 17, 116 (2015). https://doi.org/10.1007/s10544-015-0016-0

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  • DOI: https://doi.org/10.1007/s10544-015-0016-0

Keywords

  • Mesenchymal stem cell
  • Microgroove
  • Cell shape
  • Zigzag