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A point-of-care testing system with Love-wave sensor and immunogold staining enhancement for early detection of lung cancer

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Abstract

It has been reported that detection of exhaled breath condensate (EBC) is available for studies of pulmonary diseases, especially lung disease. In order to detect lung cancer (LC) at early stage, a point-of-care testing system suitable for measurement of tumor markers in EBC is developed. The assay, based on gold nanoparticle sandwich immunoassay and subsequent gold staining, was performed on a Love-wave sensor packaged inside a chip cartridge. Benefit from high sensitivity of Love-wave sensor, oriented immobilization of coating antibodies and immunogold staining enhancement, the present immunosensor could provide a sensitive, specific and rapid measurement. Carcinoembryonic antigen (CEA), neuron specific enolase (NSE) and squamous cell carcinoma antigen (SCC) in EBC collected from 17 patients with LC and 13 healthy volunteers were detected by this system. Results were compared with commercial chemiluminescence immunoassay and showed high correlation between two methods. Additionally, it revealed significantly statistical differences existing between two groups of subjects. These results indicate that the present system is suitable for detection of tumor markers in EBC and could be used as assistant tools for early detection of LC.

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Abbreviations

Ab1:

Specific coating antibody

Ab2:

Specific detecting antibody

AuNPs:

Gold nanoparticles

BSA:

Bovine serum albumin

CEA:

Carcinoembryonic antigen

CLI:

Chemiluminescence immunoassay

CT:

Computerized tomography

EBC:

Exhaled breath condensate

IDTs:

Interdigital transducers

LC:

Lung cancer

NSE:

Neuron specific enolase

PBS:

Phosphate buffered saline

PDMS:

Polydimethylsiloxane

PECVD:

Plasma enhanced chemical vapour deposition

PMMA:

Polymethylmethacrylate

QCM:

Quartz crystal microbalance

SAW:

Surface acoustic wave

SCC:

Squamous cell carcinoma antigen

SPA:

Staphylococcal protein A

SPR:

Surface plasmon resonance

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Acknowledgments

This work was supported by the following grants: National Natural Science Foundation of China No. 61320106002 to PW, No. 81201166 to XC, Research Fund for the Doctoral Program of Education Ministry of China No. 20120101130011 to PW, No. 20120101120165 to XC, Zhejiang Key Science and Technology Innovation Team 2011R50018 to XC.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027, Hangzhou, China

    Yingchang Zou, Xi Zhang, Chao An & Ping Wang

  2. Zhejiang Sir Run Run Shaw Hospital, Department of Medicine, Zhejiang University, 310027, Hangzhou, China

    Chunxue Ran & Kejing Ying

  3. Department of Biomedical Engineering, Zhouyiqing Building, Yuquan Campus, Zhejiang University, 310027, Hangzhou, China

    Ping Wang

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  1. Yingchang Zou
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  2. Xi Zhang
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  3. Chao An
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  4. Chunxue Ran
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  5. Kejing Ying
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  6. Ping Wang
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Correspondence to Ping Wang.

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Zou, Y., Zhang, X., An, C. et al. A point-of-care testing system with Love-wave sensor and immunogold staining enhancement for early detection of lung cancer. Biomed Microdevices 16, 927–935 (2014). https://doi.org/10.1007/s10544-014-9897-6

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  • DOI: https://doi.org/10.1007/s10544-014-9897-6

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