Abstract
Microfluidic impedance sensor has been introduced as a cost effective platform in biological cell sensing and counting since several decades ago. Conventional microfluidic impedance sensor usually requires the patterned gold electrodes directly in contact with the carrying buffer to measure the electrical current change due to the blockage of cells. However, patterning metal electrode probes on the silicon or glass substrate is a non-trivial task, which increases the fabrication cost of the impedance sensor. In this paper, we demonstrate an alternating current (AC) impedance based microfluidic cytometer built on a printed circuit board (PCB) coated with polydimethylsiloxane (PDMS) thin film. In addition, circulating tumor cells (Hela cells) are used to successfully demonstrate the feasibility of the microfluidic AC impedance sensor in tumor cell detection. The electrodes pre-deposited PCB costs less than US$2.00 and is widely available in the market. This device has a good potential for point-of-care diagnosis in resource-poor settings.
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References
S.K. Arya, B.L. Lim, A.R.A. Rahman, Lab Chip 13, 1995 (2013)
W. Asghar, Y. Wan, A. Ilyas, R. Bachoo, Y.T. Kim, S.M. Iqbal, Lab Chip 12, 2345 (2012)
H. Bayley, C.R. Martin, Chem. Rev. 100, 2575–2594 (2000)
J. Chen, Y. Zheng, Q.Y. Tan, Y.L. Zhang, J. Li, W.B. Geddie, M.A.S. Jewett, Y. Sun, Biomicrofluidics 5, 014113 (2011)
H. Choi, K.B. Kim, C.S. Jeon, I. Hwang, S. Lee, H.K. Kim, H.C. Kim, T.D. Chung, Lab Chip 13, 970 (2013)
G. Damhorst, B.M. Venkatesan, S. Banerjee, V. Solovyeva, R. Bashir, Biophys. J. 102, 584a (2012)
C. Dekker, Nat. Nanotechnol. 2, 209 (2007)
S. Emaminejad, M. Javanmard, R.W. Dutton, R.W. Davis, Lab Chip 12, 4499 (2012)
J. Guo, W. Lei, X. Ma, P. Xue, Y. Chen, Y. Kang, IEEE Trans. Biomed. Circ. Syst. 8, 35 (2014)
A.V. Jagtiani, J. Zhe, J. Hu, J. Carletta, Meas. Sci. Technol. 17, 1706 (2006)
J.S. Lorenz, O. Oliver, C. Catalin, G. Joanne, F.K. Ulrich, Nano Lett. 10, 2493 (2010)
M.E. Piyasena, S.W. Graves, Lab Chip 14, 1044 (2014)
H. Song, Y. Wang, J.M. Rosano, B. Prabhakarpandian, C. Garson, K. Pant, E. Lai, Lab Chip 13, 2300 (2013)
Y.N. Wang, Y. Kang, D. Xu, C.H. Chon, L. Barnett, S.A. Kalams, D. Li, Lab Chip 8, 309 (2008)
Y. Wu, D.B. James, A. Mahmoud, Biomed. Microdevices 14, 739 (2012)
X. Xuan, J. Zhu, C. Church, Microfluid. Nanofluid. 9, 1 (2010)
Y. Zheng, E. Shojaei-Baghini, A. Azad, C. Wang, Y. Sun, Lab Chip 12, 2560 (2012)
Y. Zheng, J. Nguyen, Y. Wang, Y. Sun, Lab Chip 13, 2464 (2013)
Acknowledgments
This research is supported by a seed grant from Sustainable Earth Office at Nanyang Technological University and Tier-1 Academic Research Fund from Singapore Ministry of Education (RG 26/11) awarded to Y.K. And J.G. wishes to gratefully acknowledge the Ph.D. research scholarship from Nanyang Technological University. The authors declare no financial or commercial conflicts of interest.
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Guo, J., Li, C.M. & Kang, Y. PDMS-film coated on PCB for AC impedance sensing of biological cells. Biomed Microdevices 16, 681–686 (2014). https://doi.org/10.1007/s10544-014-9872-2
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DOI: https://doi.org/10.1007/s10544-014-9872-2