Abstract
Microfluidic systems can better control cellular microenvironments and therefore are increasingly used for cell migration research. However, most existing systems are impractical to use without specialized facilities and researchers. Toward removing this barrier, we developed a compact USB microscope-based Microfluidic Chemotaxis Analysis System (UMCAS). This system integrates microfluidic devices, live cell imaging, environmental control and data analysis to provide a solution for rapid microfluidic cell migration and chemotaxis experiments with real-time result reporting. This developed system was successfully validated by testing neutrophil chemotaxis.
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Acknowledgments
This study is supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to F. Lin. We thank The Clinical Institute of Applied Research and Education at the Victoria General Hospital, Winnipeg, Manitoba, Canada, for helping with managing blood samples. We thank Abeer Saati and Dr. Sean McKanna for helping with neutrophil isolation, Andrew Pankewycz for helping design and fabricate the box for UMCAS, and Dr. Ling Zhu for gifts of cutom-sized PDMS punchers. F. Lin thanks the Winnipeg Rh Institute Foundation and the University of Manitoba for a Rh Award; J.D. Wu and J. Li thank the Manitoba Health Research Council (MHRC) for graduate fellowships; N. Wadhawan thanks the Faculty of Science at the University of Manitoba for an undergraduate summer research award.
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Michael Zhang and Francis Lin co-senior author
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Wu, J., Ouyang, L., Wadhawan, N. et al. A compact microfluidic system for cell migration studies. Biomed Microdevices 16, 521–528 (2014). https://doi.org/10.1007/s10544-014-9854-4
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DOI: https://doi.org/10.1007/s10544-014-9854-4