Skip to main content
Log in

MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript


In this work, antitumor compounds, lactoferrin [recombinant iron-free (Apo-rLf)], cisplatin (Cis) or their combination were embedded within a biodegradable polycaprolactone (PCL) polymer thin film, by a modified approach of a laser-based technique, matrix-assisted pulsed laser evaporation (MAPLE). The structural and morphological properties of the deposited hybrid films were analyzed by Fourier-transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). The in vitro effect on the cells’ morphology and proliferation of murine melanoma B16-F10 cells was investigated and correlated with the films’ surface chemistry and topography. Biological assays revealed decreased viability and proliferation, lower adherence, and morphological modifications in the case of melanoma cells cultured on both Apo-rLf and Cis thin films. The antitumor effect was enhanced by deposition of Apo-rLf with Cis within the same film. The unique capability of the new approach, based on MAPLE, to embed antitumor active factors within a biodegradable matrix for obtaining novel biodegradable hybrid platform with increased antitumor efficiency has been demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others


  • F. Bloisi, L. Vicari, R. Papa, V. Califano, R. Pedrazzani, E. Bontempi, L. Depero, Mater. Sci. Eng. C 27, 1185 (2007)

    Article  Google Scholar 

  • A.P. Caricato, A. Luches, R. Rella, Sensors 9, 2682 (2009)

    Article  Google Scholar 

  • A.P. Caricato, M. Cesaria, G. Gigli, A. Loiudice, A. Luches, M. Martino, V.V. Resta, A. Rizzo, A. Taurino, Appl. Phys. Lett. (2012)

  • D. Chrisey, A. Pique, R. McGill, J. Horwitz, B. Ringeisen, D. Bubb, P. Wu, Chem. Rev. 103, 553 (2003)

    Article  Google Scholar 

  • C. Conesa, M. Calvo, L. Sanchez, Biotechnol. Adv. 28, 831 (2010)

    Article  Google Scholar 

  • C. Constantinescu, A. Papavlu, A. Rotaru, P. Florian, F. Chelu, V. Dinca, A. Roseanu, M. Dinescu, Appl. Surf. Sci. 255, 5491 (2009)

    Article  Google Scholar 

  • L. Crino, G.V. Scagliotti, S. Ricci, F. De Marinis, M. Rinaldi, C. Gridelli, A. Ceribelli, R. Bianco, M. Marangolo, F. Di Costanzo et al., J. Clin. Oncol. Off. J. Am. Soc. Clin. Oncol. 17, 3522 (1999)

    Google Scholar 

  • Y. Kimoto, M. Nishinohara, A. Sugiyama, A. Haruna, T. Takeuchi, J. Vet. Med. Sci. Jpn. Soc. Vet. Sci. 75, 159 (2013)

    Article  Google Scholar 

  • D. Legrand, A. Pierce, E. Elass, M. Carpentier, C. Mariller, J. Mazurier, Adv. Exp. Med. Biol. 606, 163 (2008)

    Article  Google Scholar 

  • S.T. McKeown, F.T. Lundy, J. Nelson, D. Lockhart, C.R. Irwin, C.G. Cowan, J.J. Marley, Oral Oncol. 42, 685 (2006)

    Article  Google Scholar 

  • K.V. Mohan, P. Gunasekaran, E. Varalakshmi, Y. Hara, S. Nagini, Cell Biol. Int. 31, 599 (2007)

    Article  Google Scholar 

  • P.W. Parodi, Curr. Pharm. Des. 13, 813 (2007)

    Article  Google Scholar 

  • A. Piqué, D. Chrisey, R. Auyeung, J. Fitz-Gerald, H. Wu, R. McGill, S. Lakeou, P. Wu, V. Nguyen, M. Duignan, Appl. Phys. A 69, S279 (1999)

    Article  Google Scholar 

  • A. Purice, J. Schou, P. Kingshott, M. Dinescu, Chem. Phys. Lett. 435, 350 (2007)

    Article  Google Scholar 

  • L. Rodrigues, J. Teixeira, F. Schmitt, M. Paulsson, H.L. Mansson, Crit. Rev. Food Sci. Nutr. 49, 203 (2009)

    Article  Google Scholar 

  • A. Roseanu, P.E. Florian, M. Moisei, L.E. Sima, R.W. Evans, M. Trif, Biometals Int. J. Role Met. Ions Biol. Biochem. Med. 23, 485 (2010)

    Article  Google Scholar 

  • L. Rusen, C. Mustaciosu, B. Mitu, M. Filipescu, M. Dinescu, V. Dinca, Appl. Surf. Sci. (2013)

  • J. Schou. Functionalized Nanoscale Materials, Devices and Systems, NATO Science for Peace and Security Series B: Physics and Biophysics ed.^eds. (Springer, Netherlands, 2008), pp. 241–256

  • W.H. Schuette, A. Groschel, M. Sebastian, S. Andreas, T. Muller, F. Schneller, S. Guetz, C. Eschbach, S. Bohnet, M.I. Leschinger, M. Reck, Clin. Lung Cancer 14, 215 (2013)

    Article  Google Scholar 

  • M. Sommariva, M. de Cesare, A. Meini, A. Cataldo, N. Zaffaroni, E. Tagliabue, A. Balsari, J. Transl. Med. 11, 25 (2013)

    Article  Google Scholar 

  • C.J. Tai, C.S. Chen, C.S. Hung, L.J. Kuo, P.L. Wei, J.F. Chiou, C.H. Hsu, H.Y. Chiou, C.H. Wu, Anticancer. Res. 32, 5501 (2012)

    Google Scholar 

  • A. Varadhachary, J.S. Wolf, K. Petrak, B.W. O'Malley Jr., M. Spadaro, C. Curcio, G. Forni, F. Pericle, Int. J. Cancer 111, 398 (2004)

    Article  Google Scholar 

  • P.P. Ward, E. Paz, O.M. Conneely, Cell. Mol. Life Sci. CMLS 62, 2540 (2005)

    Article  Google Scholar 

  • R.P. Wernyj, P.J. Morin, Drug Resist. Updat. Rev. Commen. Antimicrob. Anticancer Chemother. 7, 227 (2004)

    Google Scholar 

  • H. Zhang, X. Feng, W. Liu, X. Jiang, W. Shan, C. Huang, H. Yi, B. Zhu, W. Zhou, L. Wang et al., J. Cell. Biochem. 112, 1832 (2011)

    Article  Google Scholar 

  • M. Zimecki, M.L. Kruzel, J. Exp. Ther. Oncol. 6, 89 (2007)

    Google Scholar 

Download references


This work was supported by the Romanian Academy project 4/2012 of IBAR and a grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN-II-RU-TE-2011-3-0289. The financial support is gratefully acknowledged. We thank Ventria Bioscience for providing the recombinant lactoferrin for this study. Sima LE gratefully acknowledges the European Social Fund POSDRU 2007–2013 through the contract POSDRU/89/1.5/S/60746.

Conflicts of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Valentina Dinca or Anca Roseanu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dinca, V., Florian, P.E., Sima, L.E. et al. MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents. Biomed Microdevices 16, 11–21 (2014).

Download citation

  • Published:

  • Issue Date:

  • DOI: