Biomedical Microdevices

, Volume 15, Issue 4, pp 699–709 | Cite as

Towards an optimal and unbiased approach for tumor cell isolation

  • Bhuvanendran Nair Gourikutty Sajay
  • Chia-Pin Chang
  • Hamizah Ahmad
  • Wong Chee Chung
  • Poenar Daniel Puiu
  • Abdur Rub Abdur Rahman
Article

Abstract

Our current understanding of clinical significance or the lack thereof of circulating tumor cells (CTCs) is biased by the technology used to isolate these rare cells. Despite the presence of a vast number of academic and commercial technologies, the lack of a standardized and optimized platform has been widely noted. We present a negative enrichment approach, integrating WBC depletion and chemical-free RBC depletion in the same setup without the need for centrifugation, washing or multiple sample handling steps. This approach achieves an average of >90 % recovery of spiked tumor cells and >99 % total WBC depletion in whole blood across multiple cell lines, in a simple and easy-to-use assay. The results presented herein and ongoing improvements aim to fulfill the need for a highly reliable, unbiased, standardized, and optimized CTC isolation platform, using component technologies that are validated for cell isolation.

Keywords

Circulating tumor cells (CTCs) Cancer cell isolation Metastasis Negative enrichment White blood cell (WBC) depletion 

Abbreviation

CTC

Circulating tumor cells

WBC

White blood cell

RBC

Red blood cell

EMT

Epithelial to mesenchymal transition

TAC

Tetrameric Antibody Complexes

GFP

Green florescent protein

PBS

Phosphate Buffered Saline

EpCAM

Epithelial cell adhesion molecule

Notes

Acknowledgments

This work was supported by the Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore under the grant number 1031490005. In addition, we greatly appreciate the support from Dr. Chaitanya Kantak for microslit membrane fabrication.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Bhuvanendran Nair Gourikutty Sajay
    • 1
    • 2
  • Chia-Pin Chang
    • 1
  • Hamizah Ahmad
    • 1
  • Wong Chee Chung
    • 1
  • Poenar Daniel Puiu
    • 2
  • Abdur Rub Abdur Rahman
    • 3
  1. 1.BioElectronics Programme, Institute of MicroelectronicsA*STAR (Agency for Science, Technology and Research)SingaporeSingapore
  2. 2.Division of Microelectronics, School of Electrical and Electronics EngineeringNanyang Technological UniversitySingaporeSingapore
  3. 3.BioElectronics Programme, Institute of MicroelectronicsA*STAR (Agency for Science, Technology and Research)SingaporeSingapore

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