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Interaction of pH-sensitive non-phospholipid liposomes with cellular mimetic membranes

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Abstract

Surfactant nanocarriers have received considerable attention in the last several years as interesting alternative to classic liposomes. Different pH-sensitive vesicular colloidal carriers based on Tween 20 derivatives, obtained after functionalization of the head groups of the surfactant with natural, or simply modified, amino acids, were proposed as drug nanocarriers. Dynamic light scattering, Small Angle X-ray Scattering, Trasmission Electron Microscopy and fluorescence studies were used for the physico-chemical characterization of vesicles and mean size, size distribution, zeta potential, vesicle morphology and bilayer properties were evaluated. The pH-sensitivity and the stability of formulations, in absence and in presence of foetal bovine serum, were also evaluated. Moreover, the contact between surfactant vesicles and liposomes designed to model the cellular membrane was investigated by fluorescence studies to preliminary explore the potential interaction between vesicle and cell membranes. Experimental findings showed that physico-chemical and technological features of pH-sensitive vesicles were influenced by the composition of the carriers. Furthermore, proposed carriers are able to interact with mimetic cell membrane and it is reasonable to attribute the observed differences in interaction to the architectural/structural properties of Tween 20 derivatives. The findings reported in this investigation showed that a deep and extensive physico-chemical characterization of the carrier is a fundamental step, according to the evidence that the knowledge of nanocarrier properties is necessary to translate its potentiality to in vitro/in vivo applications.

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References

  • R. Abes, H.M. Moulton, P. Clair, S.T. Yang, S. Abes, K. Melikov, P. Prevot, D.S. Youngblood, P.L. Iversen, L.V. Chernomordik, B. Lebleu, Nucleic Acids Res. 36, 6343 (2008)

    Article  Google Scholar 

  • H. Amenitsch, M. Rappolt, M. Kriechbaum, H. Mio, P. Laggner, S. Bernstorff, J. Synchrotron. Radiat. 5, 506 (1998)

    Article  Google Scholar 

  • H. Amenitsch, G. Caracciolo, P. Foglia, V. Fuscoletti, P. Giansanti, C. Marianecci, D. Pozzi, A. Laganà, Colloids Surf. B-Biointerfaces 82, 141 (2011)

    Article  Google Scholar 

  • A.J. Baillie, A.T. Florence, L.R. Hume, G.T. Muirhead, A. Rogerson, J. Pharm. Pharmacol. 37, 863 (1985)

    Article  Google Scholar 

  • D. Bei, J. Meng, B.B. Youan, Nanomedicine 5, 1385 (2010)

    Article  Google Scholar 

  • M. Brecht, H. Degroot, Aminoacids 6, 25 (1994)

    Google Scholar 

  • E. Canel, A. Gultepe, A. Dogan, E. Kiliç, J. Sol. Chem. 5, 5 (2006)

    Article  Google Scholar 

  • G. Caracciolo, G. Mancini, C. Bombelli, R. Caminiti, Chem. Phys. Lett. 386, 76 (2004)

    Article  Google Scholar 

  • M. Carafa, C. Marianecci, G. Lucania, E. Marchei, E. Santucci, J. Contr. Rel. 95, 67 (2004)

    Article  Google Scholar 

  • M. Carafa, L. Di Marzio, C. Marianecci, B. Cinque, G. Lucania, K. Kajiwara, M.G. Cifone, E. Santucci, Eur. J. Pharm. Sci. 28, 385 (2006)

    Article  Google Scholar 

  • E.J. Carvalho, M.C. dos Santos, ACS Nano. 4, 765 (2010)

    Article  Google Scholar 

  • J.A. Champion, Y.K. Katare, S. Mitragotri, J. Contr. Rel. 121, 3 (2007)

    Article  Google Scholar 

  • H.T. Clarke, H.B. Gillespie, S.Z. Weisshauss, J. Am. Chem. Soc. 55, 4571 (1933)

    Article  Google Scholar 

  • P. Couvreur, C. Vauthier, Pharm. Res. 23, 1417 (2006)

    Article  Google Scholar 

  • P. Decuzzi, B. Godin, T. Tanaka, S.Y. Lee, C. Chiappini, X. Liu, M. Ferrari, J. Contr. Rel. 141, 320 (2010)

    Article  Google Scholar 

  • L. Di Marzio, C. Marianecci, B. Cinque, M. Nazzarri, A.C. Cimini, L. Cristiano, M.G. Cifone, F. Alhaique, M. Carafa, Biochim. Biophys. Acta 1778, 2749 (2008)

    Article  Google Scholar 

  • L. Di Marzio, C. Marianecci, M. Petrone, F. Rinaldi, M. Carafa, Colloids Surf. B-Biointerfaces 82, 18 (2011)

    Article  Google Scholar 

  • D.E. Discher, A. Eisenberg, Science 297, 967 (2002)

    Article  Google Scholar 

  • V. Djinović, M. Momčilović, S. Grgurić-Šipka, V. Trajković, M. Mostarica Stojković, D. Miljković, T. Sabo, J. Inorg. Biochem. 98, 2168 (2004)

    Article  Google Scholar 

  • A. Dogan, N. Aslan, E. Canel, E. Kiliç, J. Sol. Chem. 39, 1589 (2010)

    Article  Google Scholar 

  • D.C. Drummond, M. Zignani, J.C. Leroux, Current status of pH-sensitive liposomes in drug delivery. Prog. Lip. Res. 39, 409 (2000)

    Article  Google Scholar 

  • E.J. Dufourc, E.J. Parish, S. Chitrakorn, I.C.P. Smith, Biochemistry 23, 6062 (1984)

    Article  Google Scholar 

  • A. El-Sayed, S. Futaki, H. Harashim, AAPS J. 11, 13 (2009)

    Article  Google Scholar 

  • W. Eschweiler, Ber. 38, 880 (1905)

    Google Scholar 

  • O.C. Farokhzad, R. Langer, ACS Nano. 3, 16 (2009)

    Article  Google Scholar 

  • Y. Hao, F. Zhao, N. Li, Y. Yang, K. Li, Int. J. Pharm. 244, 73 (2002)

    Article  Google Scholar 

  • H.E. Hofland, R. van der Geest, H.E. Bodde, H.E. Junginger, J.A. Bouwstra, Pharm. Res. 11, 659 (1994)

    Article  Google Scholar 

  • M. Hossann, M. Wiggenhorn, A. Schwerdt, K. Wachholz, N. Teichert, H. Eibl, R.D. Issels, L.H. Lindner, Biochim. Biophys. Acta 1768, 2491 (2007)

    Article  Google Scholar 

  • G. Hungerford, E.M. Castanheira, A.L. Baptista, P.J. Coutinho, M.E. Oliveira, J. Fluoresc. 15, 835 (2005)

    Article  Google Scholar 

  • K. Ishihara, R. Tsujino, M. Hamada, N. Toyoda, Y. Iwasaki, Colloids Surf. B-Biointerfaces 25, 325 (2002)

    Article  Google Scholar 

  • M. Johnsson, A. Wagenaar, J.B.F.N. Engberts, J. Am. Chem. Soc. 125, 757 (2003)

    Article  Google Scholar 

  • K. Kano, J.H. Fendler, Biochim. Biophys. Acta 509, 289 (1978)

    Article  Google Scholar 

  • J.M. Lehn, Science 295, 2400 (2002)

    Article  Google Scholar 

  • B.R. Lentz, Chem. Phys. Lipids 50, 171 (1989)

    Article  Google Scholar 

  • A. Manconi, A.O. Vila, C. Sinico, J. Figueruelo, F. Molina, A.M. Fadda, J. Drug Deliv. Sci. Technol. 16, 141 (2006)

    Google Scholar 

  • A. Manosroi, P. Wongtrakul, J. Manosroi, H. Sakai, F. Sugawara, M. Yuasa, M. Abe, Colloids Surf. B-Biointerfaces 30, 129 (2003)

    Article  Google Scholar 

  • C. Marianecci, D. Paolino, C. Celia, M. Fresta, M. Carafa, F. Alhaique, J. Contr. Rel. 147, 127 (2010)

    Article  Google Scholar 

  • A. Masotti, P. Vicennati, A. Alisi, C. Marianecci, F. Rinaldi, M. Carafa, G. Ortaggi, Bioorg. Med. Chem. Lett. 20, 3021 (2010)

    Article  Google Scholar 

  • F.M. Menger, K.D. Gabrielson, Angew Chem. Int. Ed. Engl. 34, 2091 (1995)

    Article  Google Scholar 

  • B. Mukherjee, B. Patra, B. Layek, A. Mukherjee, Int. J. Nanomedicine 2, 213 (2007)

    Google Scholar 

  • R.H. Müller, C. Jacobs, O. Kayser, Adv. Drug Deliv. Rev. 47, 3 (2001)

    Article  Google Scholar 

  • R. Muzzalupo, L. Tavano, S. Trombino, R. Cassano, N. Picci, C. La Mesa, Colloid Surf. B-Biointerfaces 64, 200 (2008)

    Article  Google Scholar 

  • S. Nie, Y. Xing, G.J. Kim, J.W. Simons, Annu. Rev. Biomed. Eng. 9, 257 (2007)

    Article  Google Scholar 

  • T. Nyholm, J.P. Slotte, Langmuir 17, 4724 (2001)

    Article  Google Scholar 

  • G. Pabst, R. Koschuch, B. Pozo-Navas, M. Rappolt, K. Lohner, P. Laggner, J. Appl. Crystallogr. 36, 1378 (2003)

    Article  Google Scholar 

  • A. Polozova, F.M. Winnik, Langmuir 15, 4222 (1999)

    Article  Google Scholar 

  • A. Ribier, R.M. Handjani-Vila, E. Bardez, B. Valeur, Colloids Surf. 10, 155 (1984)

    Article  Google Scholar 

  • E.S. Rowe, F. Zhang, T.W. Leung, J.S. Parr, P.T. Guy, Biochemistry 37, 2430 (1998)

    Article  Google Scholar 

  • M. Shinitzky, Y. Barenholz, Biochim. Biophys. Acta 515, 367 (1978)

    Article  Google Scholar 

  • A.M. Smith, H. Duan, A.M. Mohs, S. Nie, Adv. Drug Deliv. Rev. 60, 1226 (2008)

    Article  Google Scholar 

  • M.C.A. Stuart, J.C. Van De Pas, J.B.F.N. Engberts, J. Phys. Org. Chem. 18, 929 (2005)

    Article  Google Scholar 

  • C. Terzano, L. Allegra, F. Alhaique, C. Marianecci, M. Carafa, Eur. J. Pharm. Biopharm. 59, 57 (2005)

    Article  Google Scholar 

  • I.F. Uchegbu, A.T. Florence, Adv. Colloid Interface Sci. 58, 1 (1995)

    Article  Google Scholar 

  • I.F. Uchegbu, S.P. Vyas, Int. J. Pharm. 172, 33 (1998)

    Article  Google Scholar 

  • D.A. Van Hal, J.A. Bouwstra, A. Van Rensen, E. Jeremiasse, T. De Vringer, H.E. Junginger, J. Colloid Interface Sci. 178, 263 (1996)

    Article  Google Scholar 

  • P. Ventakasen, Y. Cheng, D. Kahne, J. Am. Chem. Soc. 116, 6955 (1994)

    Article  Google Scholar 

  • M.R. Vist, J.H. Davis, Biochemistry 29, 451 (1990)

    Article  Google Scholar 

  • A.T. Yoshioka, B.B. Sternberg, A.T. Florence, Int. J. Pharm. 105, 1 (1994)

    Article  Google Scholar 

  • X. Zhao, Curr. Opin. Colloid Interface Sci. 14, 340 (2009)

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Dr. Heinz Amenitsch, at the Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences (Graz—Austria), for his support in SAXS experiments, as well as for stimulating suggestions and discussion.

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Authors and Affiliations

  1. Department of Drug Chemistry and Technologies, University of Rome “Sapienza”, Rome, Italy

    Carlotta Marianecci, Federica Rinaldi & Maria Carafa

  2. Department of Pharmacy, University “G. d’Annunzio”, Chieti, Italy

    Luisa Di Marzio & Christian Celia

  3. Department of Molecular Medicine, University of Rome “Sapienza”, Rome, Italy

    Daniela Pozzi & Giulio Caracciolo

  4. Disteba, University of Salento, Lecce, Italy

    Daniela Manno & Luciana Dini

  5. Department of Health Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy

    Donatella Paolino

  6. Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA

    Christian Celia

  7. Department of Drug Chemistry and Technologies, Sapienza Università di Roma, Piazzale A. Moro, 5, 00185, Rome, Italy

    Maria Carafa

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  1. Carlotta Marianecci
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  2. Federica Rinaldi
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  10. Maria Carafa
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Correspondence to Maria Carafa.

Additional information

Carlotta Marianecci and Federica Rinaldi equally contributed to the work.

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Marianecci, C., Rinaldi, F., Di Marzio, L. et al. Interaction of pH-sensitive non-phospholipid liposomes with cellular mimetic membranes. Biomed Microdevices 15, 299–309 (2013). https://doi.org/10.1007/s10544-012-9731-y

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