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Bubble Jet agent release cartridge for chemical single cell stimulation

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Abstract

We present a new method for the distinct specific chemical stimulation of single cells and small cell clusters within their natural environment. By single-drop release of chemical agents with droplets in size of typical cell diameters (d <30 μm) on-demand micro gradients can be generated for the specific manipulation of single cells. A single channel and a double channel agent release cartridge with integrated fluidic structures and integrated agent reservoirs are shown, tested, and compared in this publication. The single channel setup features a fluidic structure fabricated by anisotropic etching of silicon. To allow for simultaneous release of different agents even though maintaining the same device size, the second type comprises a double channel fluidic structure, fabricated by photolithographic patterning of TMMF. Dispensed droplet volumes are V = 15 pl and V = 10 pl for the silicon and the TMMF based setups, respectively. Utilizing the agent release cartridges, the application in biological assays was demonstrated by hormone-stimulated premature bud formation in Physcomitrella patens and the individual staining of one single L 929 cell within a confluent grown cell culture.

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References

  • H. Andersson, A. van den Berg, Lab. Chip 4(2), 98–103 (2004)

    Article  Google Scholar 

  • H. Andersson, A. van den Berg, Lab. Chip 7, 544–546 (2007)

    Article  Google Scholar 

  • T. Boland, T. Xu, B. Damon, X. Cui, Biotechnol. J. 1, 910–917 (2006)

    Article  Google Scholar 

  • S. Boyden, J. Exp. Med. 115(3), 453–466 (1962)

    Article  Google Scholar 

  • V. Braun, K. Rehn, Eur. J. Biochem. 10, 426–438 (1969)

    Article  Google Scholar 

  • Cellectricon, Dynaflow®Pro II System – High Quality Automated Patch Clamping (Cellectricon AB, Mölndal, Sweden). http://www.cellectricon.se. Accessed Feb. 2011

  • B. Dadacz-Narloch et al., Plant Cell 23, 2696–2707 (2011)

    Article  Google Scholar 

  • E.L. Decker, W. Frank, E. Sarnighausen, R. Reski, Plant Biol. 8, 397–405 (2006)

    Article  Google Scholar 

  • H.G. Drexler, et al. DSMZ Catalogue of Human and Animal Cell Lines, Eighth Edition. (2001)

  • U. Egert et al., Brain Res. Protocol. 2, 229–242 (1998)

    Article  Google Scholar 

  • J. El-Ali et al., Anal. Chem. 77, 3629–3636 (2005)

    Article  Google Scholar 

  • R. Elmqvist, Measuring instrument of the recording type, US Patent 2566443, 1951

  • Eppendorf, Microinjectors FemtoJet and FemtoJet express Product Sheet (Eppendorf AG Hamburg, Germany). http://www.eppendorf.de. Accessed Feb. 2011

  • P. Jonas, Fast application of agonists to isolated membrane patches. In Single-Channel Recording, eds. B. Sakmann & E. Neher (Plenum press, 1995), pp. 231–243

  • T.M. Keenan, A. Folch, Lab. Chip 8, 34–57 (2008)

    Article  Google Scholar 

  • T. Kraus et al., Lab. Chip 6, 218–229 (2006)

    Article  Google Scholar 

  • H.P. Le, J. Imag. Sci. Tech. 42, 49–62 (1998)

    Google Scholar 

  • L. Leoni, D. Attiah, T.A. Desai, Sensors 2, 111–120 (2002)

    Article  Google Scholar 

  • X. Liu et al., (IEEE Trans. Rehabil. Eng. 7(3), 315–326 (1999)

    Article  Google Scholar 

  • J. Noolandi et al., Biomed. Microdev. 5(3), 195–199 (2003)

    Article  Google Scholar 

  • Pam3Cys-SKKKK(Rhodamin), (EMC microcollections GmbH, Tübingen, Germany). http://www.microcollections.de. Accessed Feb. 2011

  • M.C. Peterman, J. Noolandi, M.S. Blumenkranz, H.A. Fishman, PNAS 101(27), 9951–9954 (2004)

    Article  Google Scholar 

  • S.A. Rani et al., Antimicrob. Agents Chemother. 49(2), 728–732 (2005)

    Article  MathSciNet  Google Scholar 

  • R. Reski, Botanica Acta 111, 1–15 (1998)

    Google Scholar 

  • R. Reski, W.O. Abel, Planta 165, 354–358 (1985)

    Article  Google Scholar 

  • E.A. Roth, T. Xu, M. Das, C. Gregory, J.J. Hickman, T. Boland, Biomaterials 25, 3707–3715 (2004)

    Article  Google Scholar 

  • J. Steigert et al., Lab. Chip 9(12), 1801–1805 (2009)

    Article  Google Scholar 

  • U. Stöhr, P. Vulto, P. Hoppe, G. Urban, H. Reinecke, Journal of Micro/Nanolithography, MEMS, and MOEMS. 7, 033009 (2008)

    Google Scholar 

  • S. Takayama, E. Ostuni, P. Leduc, K. Naruse, D.E. Ingber, G.M. Whitesides, Nature 411(6841), 1016 (2001)

    Article  Google Scholar 

  • D.A. Wagenaar, J. Pine, S.M. Potter, J. Neurosci. Met. 138, 27–37 (2004)

    Article  Google Scholar 

  • N. Wangler et al., Proceedings of Thirteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences November 1–5. (2009), 679–81

  • N. Wangler et al., J. Micromech. Microeng. 21(095009), 9 (2011)

    Google Scholar 

  • S. Zibek et al., Biophys. J. 92(1), L04–L06 (2006)

    Article  Google Scholar 

Download references

Acknowledgments

We gratefully thank the German Research Foundation (DFG, ZE 527/4 and EXC 294) for financial support of this project. We also appreciate the good cooperation with the Cleanroom Service Center at IMTEK.

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Authors and Affiliations

  1. BIOSS – Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany

    N. Wangler, M. Welsche, M. Blazek, R. Reski, J. Steigert, G. Roth & R. Zengerle

  2. Laboratory for MEMS Applications, Department of Microsystems Engineering – IMTEK, University of Freiburg, Freiburg, Germany

    N. Wangler, M. Welsche, M. Blazek, R. Zengerle & N. Paust

  3. Laboratory for Process Technology, Department of Microsystems Engineering – IMTEK, University of Freiburg, Freiburg, Germany

    C. Müller & H. Reinecke

  4. Plant Biotechnology, Faculty of Biology, University of Freiburg, Freiburg, Germany

    M. Blessing, M. Vervliet-Scheebaum & R. Reski

  5. FRIAS – Freiburg Institute for Advanced Studies, University of Freiburg, Freiburg, Germany

    R. Reski

Authors
  1. N. Wangler
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  2. M. Welsche
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  3. M. Blazek
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  4. M. Blessing
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  5. M. Vervliet-Scheebaum
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  6. R. Reski
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  7. C. Müller
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  8. H. Reinecke
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  9. J. Steigert
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  10. G. Roth
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  11. R. Zengerle
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  12. N. Paust
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Correspondence to N. Wangler.

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Wangler, N., Welsche, M., Blazek, M. et al. Bubble Jet agent release cartridge for chemical single cell stimulation. Biomed Microdevices 15, 1–8 (2013). https://doi.org/10.1007/s10544-012-9681-4

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  • DOI: https://doi.org/10.1007/s10544-012-9681-4

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