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Biomedical Microdevices

, Volume 14, Issue 5, pp 921–928 | Cite as

Formation of lipid bilayers inside microfluidic channel array for monitoring membrane-embedded nanopores of phi29 DNA packaging nanomotor

  • Joon S. Shim
  • Jia Geng
  • Chong H. AhnEmail author
  • Peixuan GuoEmail author
Article

Abstract

An efficient method to form lipid bilayers inside an array of microfluidic channels has been developed and applied to monitor the membrane-embedded phi29 DNA packaging motor with an electrochemical characterization on a lab-on-a-chip (LOC) platform. A push-pull junction capturing approach was applied to confine a small amount of the lipid solution inside a microchannel. The selective permeability between solvents and water in PDMS was utilized to extract the solvent from the lipid solution, resulting in a self-formation of the lipid bilayer in the microchannel array. Each microchannel was independently connected to a silver/silver chloride (Ag/AgCl) electrode array, leading to a high-throughput monitoring of the nanopore insertion in the formed lipid bilayers. The formation of multiple lipid bilayers inside an array of microchannels and the simultaneous electrical and optical monitoring of multiple bilayer provides an efficient LOC platform for the further development of single phi29 motor pore sensing and high throughput single pore dsDNA sequencing.

Keywords

Lipid bilayer Push-pull junction capturing Nano Channel Single Pore Conductance High throughput DNA sequencing Viral DNA packaging Motor 

Notes

Acknowledgments

Supported by NIH grants EB012135 (P. G.) as well as NIH Nanomedicine Development Center: Phi29 DNA Packaging Motor for Nanomedicine, through the NIH Roadmap for Medical Research (PN2 EY 018230) (P.G.). P.G. is a co-founder of Kylin Therapeutics, Inc, and Biomotor and Nucleic Acid Nanotechnology Development Corp, Ltd.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.College of Engineering and Applied ScienceUniversity of CincinnatiCincinnatiUSA

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