Abstract
The essential step for HIV spreading limitation is the screening tests. However, there are multiple disadvantages in current screening assays which need further confirmation test. Herein we developed a rapid HIV assay combining screening and confirmation test by using the microfluidic network assay. Meanwhile, the assay is accelerated by bypassing the step of blocking. We call this method as microfluidic assay without blocking (MAWB). Both the limit of detection and reagent incubation time of MAWB are determined by screening of one model protein pair: ovalbumin and its antibody. The assay time is accelerated about 25% while the limit of detection (LOD) is well kept. Formatting the method in for both HIV screening (testing 8 HIV-related samples) and confirmation (assaying 6 kinds of HIV antibodies of each sample) within 30 min was successful. Fast HIV screening and confirmation of 20 plasma samples were also demonstrated by this method. MAWB improved the assay speed while keeping the LOD of conventional ELISA. Meanwhile, both the accuracy and throughput of MAWB were well improved, which made it an excellent candidate for a quick HIV test for both screening and confirmation. Methods like this one will find wide applications in clinical diagnosis and biochemical analysis based on the interactions between pairs of molecules.
Similar content being viewed by others
References
A. Bernard, B. Michel, E. Delamarche, Anal Chem 73, 8 (2001)
N. Choi, M. Cabodi, B. Held, J. Gleghorn, L. Bonassar, A. Stroock, Nat Mater 6, 908 (2007)
D. Duffy, J. McDonald, O. Schueller, G. Whitesides, Anal Chem 70, 4974 (1998)
J. Hermez, J. Petrak, M. Karkouri, G. Riedner, AIDS 24, 25 (2010)
D. Huh, K. Milis, X. Zhu, A. Burn, M. Thouless, S. Takayama, Nat Mater 6, 424 (2007)
X. Jiang, J. Ng, A. Stroock, S. Dertinger, G. Whitesides, J Am Chem Soc 125, 5294 (2003)
R. Lequin, Clin Chem 51(12), 2415 (2005)
F. Ligler, K. Sapsford, J. Golden, L. Lake, C. Taitt, M. Dyer, S. Barone, C. Myatt, J Anal Sci 23, 5 (2007)
Y. Liu, Y. Sun, K. Sun, L. Song, X. Jiang, J Mater Chem 20, 7305 (2010)
Y. Liu, J. Yu, M. Du, W. Wang, W. Zhang, Z. Wang, X. Jiang, Biomed Microdev (2011). doi:10.1007/s10544-011-9581-z
W. Pan, W. Chen, X. Jiang, Anal Chem 82, 3974 (2010)
R. Petty, H. Li, J. Maduram, R. Ismagilov, M. Mrksich, J Am Chem Soc 129, 8966 (2007)
P. Pozzilli, U. Mario, Diabetes Care 24, 1460 (2001)
J. Su, M. Bringer, R. Ismagilov, M. Mrksich, J Am Chem Soc 127, 7280 (2005)
Y. Sun, Y. Liu, W. Qu, X. Jiang, Anal Chim Acta 650, 98 (2009)
D. Trono, C. Lint, C. Rouzioux, E. Verdin, F. Barré-Sinoussi, T. Chun, N. Chomont, Science 329, 174 (2010)
B. Vahidi, J. Park, H. Kim, N. Jeon, J Neurosci Meth 170, 188 (2008)
J.B. Wang, Y. Zhou, H.W. Qiu, H. Huang, C.H. Sun, J.Z. Xi, Y.Y. Huang, Lab Chip 9, 1831 (2009)
G. Whitesides, Nature 442, 368 (2006)
Y. Xia, G. Whitesides, Angew Chem Int Ed 37, 550 (1998)
P. Yager, T. Edwards, E. Fu, K. Helton, K. Nelson, M. Tam, B. Weigl, Nature 442, 412 (2006)
D. Yang, X. Niu, Y. Liu, Y. Wang, X. Gu, L. Song, R. Zhao, L. Ma, Y. Shao, X. Jiang, Adv Mater 20, 1 (2008)
Acknowledgments
We thank the Ministry of Science and Technology(2006AA03Z323, 2009CB930001), the Chinese Academy of Science, (KJCX2-YW-M15) the National Natural Science Foundation of China(20890020, 90813032), and the Ministry of Health of China (2008ZX10001-013) for funding.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Song, L., Zhang, Y., Wang, W. et al. Microfluidic assay without blocking for rapid HIV screening and confirmation. Biomed Microdevices 14, 631–640 (2012). https://doi.org/10.1007/s10544-012-9644-9
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10544-012-9644-9