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Biomedical Microdevices

, Volume 13, Issue 6, pp 973–982 | Cite as

Electrical detection of dsDNA and polymerase chain reaction amplification

  • Eric Salm
  • Yi-Shao Liu
  • Daniel Marchwiany
  • Dallas Morisette
  • Yiping He
  • Arun K. Bhunia
  • Rashid Bashir
Article

Abstract

Food-borne pathogens and food safety-related outbreaks have come to the forefront over recent years. Estimates on the annual cost of sicknesses, hospitalizations, and deaths run into the billions of dollars. There is a large body of research on detection of food-borne pathogens; however, the widely accepted current systems are limited by costly reagents, lengthy time to completion, and expensive equipment. Our aim is to develop a label-free method for determining a change in DNA concentration after a PCR assay. We first used impedance spectroscopy to characterize the change in concentration of purified DNA in deionized water within a microfluidic biochip. To adequately measure the change in DNA concentration in PCR solution, it was necessary to go through a purification and precipitation step to minimize the effects of primers, PCR reagents, and excess salts. It was then shown that the purification and precipitation of the fully amplified PCR reaction showed results similar to the control tests performed with DNA in deionized water. We believe that this work has brought label free electrical biosensors for PCR amplification one step closer to reality.

Keywords

Label-free Electrical detection PCR DNA Listeria 

Notes

Acknowledgements

We acknowledge funding support from a cooperative agreement with Purdue University and the Agricultural Research Service of the United States Department of Agriculture, project number 1935-42000-035, and a sub-contract to the University of Illinois at Urbana-Champaign.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Eric Salm
    • 1
    • 2
  • Yi-Shao Liu
    • 4
  • Daniel Marchwiany
    • 2
    • 3
  • Dallas Morisette
    • 5
    • 6
  • Yiping He
    • 8
  • Arun K. Bhunia
    • 7
  • Rashid Bashir
    • 1
    • 2
    • 9
  1. 1.Department of BioengineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Micro and Nanotechnology LaboratoryUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Department of Molecular and Cellular BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.Taiwan Semiconductor Manufacturing CorporationHsinchuRepublic of China
  5. 5.BioVitesse, Inc.West LafayetteUSA
  6. 6.Birck Nanotechnology CenterPurdue UniversityWest LafayetteUSA
  7. 7.Department of Food SciencePurdue UniversityWest LafayetteUSA
  8. 8.USDA-ARS-ERRCWyndmoorUSA
  9. 9.Department of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignIllinoisUSA

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