Continuous high-throughput phosphopeptide enrichment using microfluidic channels modified with aligned ZnO/TiO2 nanorod arrays
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A capillary microchannel (CM) containing TiO2-coated ZnO nanorod arrays was applied as a novel microfluidic device to selectively bind and enrich phosphopeptides. The device was prepared by pumping a TiO2 sol into a CM containing preformed ZnO nanorod arrays. Different thicknesses of the TiO2 coating were obtained by controlling the flow duration of TiO2 sol. The modified CM achieved uninterrupted high-throughput introduction, capture and enrichment of phosphopeptides using continuous-flow operation. The microfluidic device based on the modified CM showed great selectivity, sensitivity and durability for the enrichment of phosphopeptides from tryptic protein digests. These results suggest that microfluidic chips employing this strategy can be used for rapid and high-throughput enrichment of phosphopeptides from complex mixtures.
KeywordsMicrofluidic device TiO2/ZnO nanorod arrays Phosphopeptide enrichment Continuous-flow High-throughput Nanobiotechnology
The authors gratefully acknowledge the financial support by Shanghai Municipal Education Commission (No.07SG37), Natural Science Foundation of China (No. 51072034), Shanghai Leading Academic Discipline Project (B603), the Cultivation Fund of the Key Scientific and Technical Innovation Project (No.708039), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning,and the Program of Introducing Talents of Discipline to Universities (No.111-2-04).
- J.Y. Yan, X.L. Li, S.Y. Cheng, Y.X. Ke, X.M. Liang, Chem. Commun. 2929–31 (2009)Google Scholar