Biomedical Microdevices

, Volume 13, Issue 3, pp 539–548 | Cite as

A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology

  • Yandong Gao
  • Devi Majumdar
  • Bojana Jovanovic
  • Candice Shaifer
  • P. Charles Lin
  • Andries Zijlstra
  • Donna J. Webb
  • Deyu Li
Article

Abstract

A versatile microfluidic platform allowing co-culture of multiple cell populations in close proximity with separate control of their microenvironments would be extremely valuable for many biological applications. Here, we report a simple and compact microfluidic platform that has these desirable features and allows for real-time, live-cell imaging of cell-cell interactions. Using a pneumatically/hydraulically controlled poly(dimethylsiloxane) (PDMS) valve barrier, distinct cell types can be cultured in side-by-side microfluidic chambers with their optimum culture media and treated separately without affecting the other cell population. The platform is capable of both two-dimensional and three-dimensional cell co-culture and through variations of the valve barrier design, the platform allows for cell-cell interactions through either direct cell contact or soluble factors alone. The platform has been used to perform dynamic imaging of synapse formation in hippocampal neurons by separate transfection of two groups of neurons with fluorescent pre- and post-synaptic protein markers. In addition, cross-migration of 4T1 tumor cells and endothelial cells has been studied under normoxic and hypoxic conditions, which revealed different migration patterns, suggesting the importance of the microenvironments in cell-cell interactions and biological activities.

Keywords

Cell culture Cell-cell interaction Synapse formation Cell migration 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Yandong Gao
    • 1
  • Devi Majumdar
    • 2
  • Bojana Jovanovic
    • 3
  • Candice Shaifer
    • 4
  • P. Charles Lin
    • 5
  • Andries Zijlstra
    • 6
  • Donna J. Webb
    • 2
    • 3
  • Deyu Li
    • 1
  1. 1.Department of Mechanical EngineeringVanderbilt UniversityNashvilleUSA
  2. 2.Department of Biological Sciences and Vanderbilt Kennedy Center for Research on Human DevelopmentVanderbilt UniversityNashvilleUSA
  3. 3.Department of Cancer BiologyVanderbilt UniversityNashvilleUSA
  4. 4.Department of Biochemistry and Cancer BiologyMeharry Medical CollegeNashvilleUSA
  5. 5.Center for Cancer ResearchNational Cancer InstituteFrederickUSA
  6. 6.Department of PathologyVanderbilt UniversityNashvilleUSA

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